Use of molecular markers to differentiate between commercial strains of the button mushroom Agaricus bisporus

Ramı́rez, Lucı́a

doi:10.1016/s0378-1097(01)00117-3

Cited by 17 publications

(10 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…BSA is an efficient and effective method for identifying markers linked to a specific gene or genomic region (27). Although BSA is widely applied in plants, especially for disease resistance (20,27,57), its application to mushrooms is less well known; the identification of markers linked with mushroom weight in A. bisporus (40), mating type factors in L. edodes (18), and the sporeless trait in P. pulmonarius (38) has been reported. This method will be a useful tool for finding markers closely linked to important agronomic traits in mushroom breeding.…”

Section: Discussionmentioning

confidence: 99%

Construction of a Genetic Linkage Map Based on Amplified Fragment Length Polymorphism Markers and Development of Sequence-Tagged Site Markers for Marker-Assisted Selection of the Sporeless Trait in the Oyster Mushroom (Pleurotus eryngii)

Okuda

Ueda

Obatake³

et al. 2012

Appl Environ Microbiol

View full text Add to dashboard Cite

A large number of spores from fruiting bodies can lead to allergic reactions and other problems during the cultivation of edible mushrooms, including Pleurotus eryngii (DC.) Quél. A cultivar harboring a sporulation-deficient (sporeless) mutation would be useful for preventing these problems, but traditional breeding requires extensive time and labor. In this study, using a sporeless P. eryngii strain, we constructed a genetic linkage map to introduce a molecular breeding program like marker-assisted selection. Based on the segregation of 294 amplified fragment length polymorphism markers, two mating type factors, and the sporeless trait, the linkage map consisted of 11 linkage groups with a total length of 837.2 centimorgans (cM). The gene region responsible for the sporeless trait was located in linkage group IX with 32 amplified fragment length polymorphism markers and the B mating type factor. We also identified eight markers closely linked (within 1.2 cM) to the sporeless locus using bulked-segregant analysis-based amplified fragment length polymorphism. One such amplified fragment length polymorphism marker was converted into two sequence-tagged site markers, SD488-I and SD488-II. Using 14 wild isolates, sequence-tagged site analysis indicated the potential usefulness of the combination of two sequence-tagged site markers in cross-breeding of the sporeless strain. It also suggested that a map constructed for P. eryngii has adequate accuracy for marker-assisted selection.

show abstract

Section: Discussionmentioning

confidence: 99%

Construction of a Genetic Linkage Map Based on Amplified Fragment Length Polymorphism Markers and Development of Sequence-Tagged Site Markers for Marker-Assisted Selection of the Sporeless Trait in the Oyster Mushroom (Pleurotus eryngii)

Okuda

Ueda

Obatake³

et al. 2012

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…As a result, mycelium growth can be influenced by a reduction or excess concentration of nutrients, accumulation of toxic products, production of secondary metabolites, and changes in factors such as pH, which consequently can influence the mushroom production (2,14,15).…”

Section: Introductionmentioning

confidence: 99%

Yield of four Agaricus bisporus strains in three compost formulations and chemical composition analyses of the mushrooms

Andrade¹,

Zied²,

Minhoni³

et al. 2008

Braz. J. Microbiol.

View full text Add to dashboard Cite

Three compost formulations, consisting of two varieties of Cynodom dactylon (L.) Pers. (Coast-cross and Tyfton) and oat (Avena sativa) straw were tested for the cultivation of A. bisporus strains ABI-01/01, ABI-04/02, ABI-05/03, and ABI-06/04. A completely randomized experimental design in a factorial scheme was adopted, with 12 treatments (4 A. bisporus strains × 3 types of compost) and 8 replicates. Each experimental unit corresponded to one box containing 12 -12.5 kg fresh wet compost. The data were submitted to analysis of variance and the means were compared by Tukey test. According to the results, productivity of mushrooms was influenced by strain and/or compost type. It was also verified that crude protein, ash, and crude fiber contents in the mushroom varied with A. bisporus strain and straw used in the formulation of the compost.

show abstract

“…If they were hybrids, such high similarity coefficient would suggest that strains No.1, No.56 and No.1023 might be re-isolated from strain U3 by different producers, whereas the genetic differences of them in this work might contribute to the mutation through recombination, disassociation and splitting of DNA during the propagation process. (Loftus et al 1988;Sonnenberg et al 1999;Ramirez et al 2001). Meanwhile, the various cultivation conditions induce the mutation of the hybrids.…”

Section: Resultsmentioning

confidence: 99%

Differentiation of commercial strains of Agaricus species in China with inter-simple sequence repeat marker

Guan

Shao

et al. 2008

World J Microbiol Biotechnol

View full text Add to dashboard Cite

In recent years, Agaricus mushroom production has undergone an important increase in the world. However, there is no consensus over the taxonomy of some commercial strains. So rapid and reliable methods to identify Agaricus strains are urgently needed. In this paper, six inter-simple sequence repeat (ISSR) primers were screened from 20 primers to analyse the genetic diversities of the 12 main commercial strains of Agaricus in China. The results showed that a total of 124 bands were produced, among which 80.6% was polymorphic, the similarity coefficients ranged from 0.69 to 0.98. Twelve tested strains were divided into 2 groups on the similarity coefficient of 0.75. Three Agaricus bitorquis strains were clustered in one group, and seven Agaricus bisporus strains were clustered with Agaricus strains S1 and S2 in the other group. As comparison with ISSR, intergenic spacers combined with restriction fragment length polymorphisms (IGS-RFLP) was employed to analyse genetic diversities of the tested strains by digesting IGS amplified products with restriction enzymes Hae III, Alu I, Rsa I, respectively, and IGS-RFLP cluster results were similar with those of ISSR. However, ISSR was superior to IGS-RFLP in differentiating closely related strains. It is suggested that the ISSR marker is an alternative method to differentiate Agaricus strains.

show abstract

Use of molecular markers to differentiate between commercial strains of the button mushroom Agaricus bisporus

Cited by 17 publications

References 15 publications

Construction of a Genetic Linkage Map Based on Amplified Fragment Length Polymorphism Markers and Development of Sequence-Tagged Site Markers for Marker-Assisted Selection of the Sporeless Trait in the Oyster Mushroom (Pleurotus eryngii)

Construction of a Genetic Linkage Map Based on Amplified Fragment Length Polymorphism Markers and Development of Sequence-Tagged Site Markers for Marker-Assisted Selection of the Sporeless Trait in the Oyster Mushroom (Pleurotus eryngii)

Yield of four Agaricus bisporus strains in three compost formulations and chemical composition analyses of the mushrooms

Differentiation of commercial strains of Agaricus species in China with inter-simple sequence repeat marker

Contact Info

Product

Resources

About