Use of Modified Silicas for Lipase Immobilization

Carvalho, Nayára Bezerra; Lima, Álvaro Silva; Soares, Cleide Mara Faria

doi:10.5935/0100-4042.20140304

Cited by 11 publications

(16 citation statements)

References 5 publications

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“…The first category is a physical method dependent on adsorption forces and electrostatic interactions between the enzyme and a matrix. Immobilization by covalent bond involves formation of a covalent bond between the terminal residues of the biomolecule and a functional group on the support, while encapsulation or microencapsulation methods refers to the entrapment of an enzyme in polymeric matrices [9,51].…”

Section: Co-immobilization Of Enzymes 21 Co-immobilization Of Enzymes Into Solid Supportsmentioning

confidence: 99%

Multicatalytic Hybrid Materials for Biocatalytic and Chemoenzymatic Cascades—Strategies for Multicatalyst (Enzyme) Co-Immobilization

et al. 2021

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During recent decades, the use of enzymes or chemoenzymatic cascades for organic chemistry has gained much importance in fundamental and industrial research. Moreover, several enzymatic and chemoenzymatic reactions have also served in green and sustainable manufacturing processes especially in fine chemicals, pharmaceutical, and flavor/fragrance industries. Unfortunately, only a few processes have been applied at industrial scale because of the low stabilities of enzymes along with the problematic processes of their recovery and reuse. Immobilization and co-immobilization offer an ideal solution to these problems. This review gives an overview of all the pathways for enzyme immobilization and their use in integrated enzymatic and chemoenzymatic processes in cascade or in a one-pot concomitant execution. We place emphasis on the factors that must be considered to understand the process of immobilization. A better understanding of this fundamental process is an essential tool not only in the choice of the best route of immobilization but also in the understanding of their catalytic activity.

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Section: Co-immobilization Of Enzymes 21 Co-immobilization Of Enzymes Into Solid Supportsmentioning

confidence: 99%

Multicatalytic Hybrid Materials for Biocatalytic and Chemoenzymatic Cascades—Strategies for Multicatalyst (Enzyme) Co-Immobilization

et al. 2021

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“…Further, depending upon the type of interactions between enzymes and carriers, irreversible and reversible types of immobilization techniques can be used . Lipase immobilization techniques have been reviewed through use of modified silicas and siliceous supports along with their applications in synthetic reactions. Recent advances in improving the enantioselectivity through medium, substrate and enzyme engineering approaches have been reviewed for free or immobilized C. antarctica lipase B .…”

Section: Major Challenges With Respect To Industrial Implementationmentioning

confidence: 99%

Recent advances on sources and industrial applications of lipases

Sarmah

Revathi

Sheelu

et al. 2017

Biotechnology Progress

296

148

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Lipases are the industrially important biocatalysts, which are envisioned to have tremendous applications in the manufacture of a wide range of products. Their unique properties such as better stability, selectivity and substrate specificity position them as the most expansively used industrial enzymes. The research on production and applications of lipases is ever growing and there exists a need to have a latest review on the research findings of lipases. The present review aims at giving the latest and broadest overall picture of research and development on lipases by including the current studies and progressions not only in the diverse industrial application fields of lipases, but also with regard to its structure, classification and sources. Also, a special emphasis has been made on the aspects such as process optimization, modeling, and design that are very critical for further scale-up and industrial implementation. The detailed tabulations provided in each section, which are prepared by the exhaustive review of current literature covering the various aspects of lipase including its production and applications along with example case studies, will serve as the comprehensive source of current advancements in lipase research. This review will be very useful for the researchers from both industry as well as academia in promoting lipolysis as the most promising approaches to intensified, greener and sustainable processes. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 34:5-28, 2018.

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“…To use enzymes in industrial processes, in many cases, it is necessary to increase their operational stability SOARES, 2015;DICOSIMO et al, 2013). Therefore, the search for biocatalysts with increased stability is one of the major challenges in the last decades.…”

Section: Introductionmentioning

confidence: 99%

“…The choice of a methodology and a suitable support for immobilization is critical to obtain a suitable insolubilized biocatalyst aiming its application at an industrial scale. For that, it is desirable that the biocatalyst is active, stable, easily recovered from the reaction medium at the reaction end, and reusable SOARES, 2015;NIKOLIĆ et al, 2020). The critical choice of an enzyme immobilization strategy is based on the efficiency of the enzyme in the desired process, the costs of the immobilization procedure, the toxicity of the immobilization reagents, and the desired final properties of the immobilized biocatalyst (DICOSIMO et al, 2013;KHARRAT et al, 2011;THANGARAJ et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…ISSN 2525-8761 (BARBOSA et al, 2016;CARVALHO, Nayara B.;SOARES, 2015;LIMA et al, 2015;VESCOVI et al, 2016). Several surface modifying agents can promote a suitable chemical, physical, and/or morphological modification of the support surface; thus, contributing to synthesize an immobilized biocatalysts with high catalytic efficiency and operational stability to fulfill the requirements of an industrial process (BARBOSA et al, 2014;BARBOSA et al, 2016;CARVALHO et al, 2013;SHELDON;VAN PELT, 2013;SOARES et al, 2006;SOUZA et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Hydrophobic immobilization of Burkholderia cepacia lipase onto octyl-silica for synthesis of flavors esters

Barbosa

Santos

Almeida

et al. 2020

BJD

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Burkholderia cepacia lipase (BCL) was immobilized onto silica modified with octyl groups (OS) and the biocatalyst (BCL-OS) was evaluated as its performance in the synthesis in organic medium (synthesis of flavor esters as a model). The maximum support loading was 0.375 genzyme/gsupport, yielding a biocatalyst with an activity of 1197 U/gsupport at pH 7.0 and 50 °C in the hydrolysis of olive oil. The biocatalyst BCL-OS showed to be 9-fold more stable than the free lipase at 60°C in buffer solution (absence of substrates), with an increase of half-life from 16 to 144 h. The physical-chemical characterization of silica, octyl silica, and BCL-OS biocatalyst allowed confirming the immobilization of BCL onto the modified silica. The biocatalyst had an excellent performance in the synthesis of flavor esters, yielding more than 85% esterification yield (based on acid consumption) for acetic and butyric acids as acyl donors, and ethanol, butanol and hexanol as acyl acceptors. The biocatalyst could be recycled by ten 5 h-cycles of butyl butyrate syntheses at 37°C in heptane, retaining around 80% of its initial activity. Therefore, these results indicate that the BCL immobilized onto silica modified with octyl groups is a promising biocatalyst for application in organic syntheses.

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Use of Modified Silicas for Lipase Immobilization

Cited by 11 publications

References 5 publications

Multicatalytic Hybrid Materials for Biocatalytic and Chemoenzymatic Cascades—Strategies for Multicatalyst (Enzyme) Co-Immobilization

Multicatalytic Hybrid Materials for Biocatalytic and Chemoenzymatic Cascades—Strategies for Multicatalyst (Enzyme) Co-Immobilization

Recent advances on sources and industrial applications of lipases

Hydrophobic immobilization of Burkholderia cepacia lipase onto octyl-silica for synthesis of flavors esters

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