Hydrophobic immobilization of Burkholderia cepacia lipase onto octyl-silica for synthesis of flavors esters

Abstract

Burkholderia cepacia lipase (BCL) was immobilized onto silica modified with octyl groups (OS) and the biocatalyst (BCL-OS) was evaluated as its performance in the synthesis in organic medium (synthesis of flavor esters as a model). The maximum support loading was 0.375 genzyme/gsupport, yielding a biocatalyst with an activity of 1197 U/gsupport at pH 7.0 and 50 °C in the hydrolysis of olive oil. The biocatalyst BCL-OS showed to be 9-fold more stable than the free lipase at 60°C in buffer solution (absence of substrates), with an increase of half-life from 16 to 144 h. The physical-chemical characterization of silica, octyl silica, and BCL-OS biocatalyst allowed confirming the immobilization of BCL onto the modified silica. The biocatalyst had an excellent performance in the synthesis of flavor esters, yielding more than 85% esterification yield (based on acid consumption) for acetic and butyric acids as acyl donors, and ethanol, butanol and hexanol as acyl acceptors. The biocatalyst could be recycled by ten 5 h-cycles of butyl butyrate syntheses at 37°C in heptane, retaining around 80% of its initial activity. Therefore, these results indicate that the BCL immobilized onto silica modified with octyl groups is a promising biocatalyst for application in organic syntheses.

Simultaneous Isomerization and Fermentation (Sif) of Sugarcane Bagasse Hydrothermal Hemicellulose Hydrolysate / Isomerização E Fermentação Simultâneas (Sif) De Hidrolisado Hemicelulósico Hidrotérmico De Bagaço De Cana

“…Some strategies can be used to improve yields and productivity in the presence of inhibitors, like the use of higher biocatalyst concentrations to increase process rate or the detoxification of the hydrolysate before fermentation. Arruda et al (2008) studied the detoxification of sugarcane bagasse hemicellulose acid hydrolysate and observed that the use of adsorption on activated carbon was effective in reducing the inhibitory compounds.…”

Immobilization of CalB Lipase by adsorption on magnetic nanoparticles: A heterogeneous biocatalysis/ Imobilização da lipase CalB por adsorção em nanopartículas magnéticas: um biocatalisador heterogêneo

“…The model reagent chosen to represent the carboxylic acids present in vegetable oils was oleic acid. The alcohol used was ethanol (EtOH) (BARBOSA et al, 2020;CANNILLA et al, 2018).…”

Influence of additives in the transesterification of crude coconut oil catalyzed by Burkholderia cepacia lipase immobilized onto agro-industrial waste fibers

“…In addition, some lipases need a certain amount of water to maintain their own conformation (LI et al, 2012). Another important aspect is that, like Burkholderia cepacia lipase (BCL), some have the catalytic site protected by a protein structure, the lid or lid, which may be closed rendering the enzyme inactive, but in the presence of the oil-water interface, the lid opens and leaves exposed the active site so that catalysis occurs (BARBOSA et al, 2020;NIGAM et al, 2014).…”