Use of maltose hydrolysis measurements to characterize the interaction between the aqueous diffusion barrier and the epithelium in the rat jejunum.

Levitt, Michael D.; Fine, C.; Furne, Julie; Levitt, David G.

doi:10.1172/jci118673

Cited by 16 publications

(11 citation statements)

References 19 publications

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“…It is important to note, that there was virtually no saturation in the rate of maltose hydrolysis (black circles, Mean ± SEM) even in the range of high concentrations of the substrate in the lumen (35 -70 mmol/l), which exceeds by many times the value of K m for maltase: 3.3 -3.7 mmol/l [7,18]. The rate of released glucose absorption did not also reach full saturation (Figure 3, open circles, Mean ± SEM).…”

Section: Maltose Hydrolysis and Glucose Absorptionmentioning

confidence: 99%

“…In both versions of the model, Michaelis constant (K m ) for maltose hydrolysis was taken equal to 3.7 mmol/l [7,16,18].…”

Section: Maltose Hydrolysis and Glucose Absorptionmentioning

confidence: 99%

“…Meanwhile, a complex architecture of the intestinal surface with the pre-epithelial layer may significantly affect the kinetics of hydrolytic and transport processes [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

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Kinetics and mechanisms of glucose absorption in the rat small intestine under physiological conditions

Груздков¹,

Громова²,

Grefner³

et al. 2012

JBPC

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Previous studies have shown two components of glucose absorption in the small intestine: a secondary active transport through SGLT1, and unsaturated component, recently attributed mainly to the facilitated diffusion through GLUT2, but the relationship between these two components under physiological conditions remains controversial. In chronic experiments on nonanesthetized rats we investigated for the first time the kinetics of maltose hydrolysis and glucose absorption in the isolated loop of the small intestine in a wide range of maltose and glucose concentrations (25 -200 mmol/l glucose). The processes were simulated on mathematical models which took into account the current views about mechanisms of hydrolysis and transport of nutrients and geometric characteristics of the intestinal surface. The results of chronic experiments and mathematical simulation have shown that under the close to physiological conditions the glucose transport mediated by SGLT1 is the main mechanism of its absorption in comparison with the unsaturated component. This was demonstrated not only at low, but also at high substrate concentrations. We conclude that correct evaluation of the relative contribution of different mechanisms in glucose transport through the intestinal epithelium requires taking into account the geometric specificities of its surface.

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Section: Maltose Hydrolysis and Glucose Absorptionmentioning

confidence: 99%

“…In both versions of the model, Michaelis constant (K m ) for maltose hydrolysis was taken equal to 3.7 mmol/l [7,16,18].…”

Section: Maltose Hydrolysis and Glucose Absorptionmentioning

confidence: 99%

See 1 more Smart Citation

Kinetics and mechanisms of glucose absorption in the rat small intestine under physiological conditions

Груздков¹,

Громова²,

Grefner³

et al. 2012

JBPC

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show abstract

“…Intestinal absorption of solute requires that the compound cross two barriers, the unstirred layer as an aqueous diffusion barrier, and epithelium which consist of enterocytes and paracellular factors [29][30][31][32][33][34] . Disaccharides are absorbed at a faster rate than monosaccharides in vivo, but inversely in vitro for the absorption of disaccharides coupled with the membrane digestion.…”

Section: Mechanism Of the Combinationmentioning

confidence: 99%

“…The maltose is hydrolyzed during it passes through the glycocalyx and enterocytes [38] . On the other hand, thickness of the unstirred layer (glycocalyx) largely depends on motility of the intestine, which becomes relatively thin when the intestine exhibits motility [29,37] . Suppressed by GA, the tension and intestinal auto-rhythmic contraction had un-negligible effects on unstirred layer not only, but also on the tight junction around the enterocytes.…”

Section: Mechanism Of the Combinationmentioning

confidence: 99%

Inhibitory effect and mechanism of acarbose combined with gymnemic acid on maltose absorption in rat intestine

Luo

Wang²,

Imoto³

et al. 2001

WJG

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Subject headings diabetes mellitus; maltose; gymnemic acid; alpha-glucosidases; intestinal mucosa; rats; nutrition Luo H, Wang LF, Imoto T, Hiji Y. Inhibitory effect and mechanism of acarbose combined with gymnemic acid on maltose absorption in rat intestine. World J Gastroenterol, 2001;7(1):9-15 Abstract AIM To compare the combinative and individual effect of acarbose and gymnemic acid (GA) on maltose absorption and hydrolysis in small intestine to determine whether nutrient control in diabetic care can be improved by combination of them. METHODS The absorption and hydrolysis of maltose were studied by cyclic perfusion of intestinal loops in situ and motility of the intestine was recorded with the intestinal ring in vitro using Wistar rats. RESULTS The total inhibitory rate of maltose absorption was improved by the combination of GA (0.1g/L -1.0g/L) and acarbose (0.1mmol/L -2.0mmol/L) throughout their effective duration (P <0.05, U test of Mann-Whitney), although the improvement only could be seen at a low dosage during the first hour. With the combination, inhibitory duration of acarbose on maltose absorption was prolonged to 3 h and the inhibitory effect onset of GA was fastened to 15 min. GA suppressed the intestinal mobility with a good correlation (r = 0.98) to the inhibitory effect of GA on maltose absorption and the inhibitory effect of 2 mmol/L (high dose) acarbose on maltose hydrolysis was dual modulated by 1 g/L GA in vivo indicating that the combined effects involved the functional alteration of intestinal barriers. CONCLUSION There are augmented effects of acarbose and GA,which involve pre-cellular and paracellular barriers. Diabetic care can be improved by employing the combination.

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The Influence of the Intestinal Unstirred Water Layers on the Understanding of the Mechanisms of Lipid Absorption

Thomson

Wild

2001

Intestinal Lipid Metabolism

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Use of maltose hydrolysis measurements to characterize the interaction between the aqueous diffusion barrier and the epithelium in the rat jejunum.

Cited by 16 publications

References 19 publications

Kinetics and mechanisms of glucose absorption in the rat small intestine under physiological conditions

Kinetics and mechanisms of glucose absorption in the rat small intestine under physiological conditions

Inhibitory effect and mechanism of acarbose combined with gymnemic acid on maltose absorption in rat intestine

The Influence of the Intestinal Unstirred Water Layers on the Understanding of the Mechanisms of Lipid Absorption

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