Use of "Lysis Buffer" in DNA isolation and its implication for museum collections

Longmire, Jonathan L.; Maltbie, Mary; Baker, Robert J.

doi:10.5962/bhl.title.143318

Cited by 329 publications

(320 citation statements)

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“…We collected contour feathers and blood samples by venipunction of the brachial vein (~40 μl). Samples were stored separately in test tubes containing 95 % ethanol (feathers) or Longmire buffer (Longmire et al 1997;blood sample), and preserved at -20 °C until analyzed. Partridges were released within 30 min in the same capture site.…”

Section: Methodsmentioning

confidence: 99%

Mitochondrial DNA and microsatellite markers evidence a different pattern of hybridization in red-legged partridge (Alectoris rufa) populations from NW Italy

et al. 2012

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Abstract.Genetic introgression with exotic genomes represents a major conservation concern for the red-legged partridge (Alectoris rufa, Phasianidae). In particular, massive releases of chukar partridges (Alectoris chukar) and/or red-legged x chukar partridge hybrids for hunting purposes have cast serious doubts on the Italian present-day occurrence of wild A. rufa populations not affected by introgressive hybridization. This study investigates the genetic structure of red-legged partridges populations in two ecologically different areas in Northern Italy. Analysis of maternal mitochondrial DNA and biparental microsatellite markers excluded the presence of hybridization in a typical agricultural habitat where hunting and release of reared birds are strictly banned. By contrast, signs of chukar introgression were detected in a perifluvial habitat unusual for the red-legged partridge in Italy. The present study documents the first red-legged partridge population with no genetic evidences of recent chukar introgression presently living in Italy. We recommend that urgent conservation actions are taken to preserve the genetic integrity of this population from the risk of hybridization with farm-reared birds and to support its long-term conservation.

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Section: Methodsmentioning

confidence: 99%

Mitochondrial DNA and microsatellite markers evidence a different pattern of hybridization in red-legged partridge (Alectoris rufa) populations from NW Italy

et al. 2012

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“…We extracted genomic DNA from skeletal muscle or organ tissue samples with standard phenol methods (Longmire et al, 1997). We followed Van Den Bussche and Hoofers (2000) methods to amplify and sequence a 2.6 kilobase-fragment of mtDNA encompassing 12S rRNA, tRNA Val , and 16S rRNA genes.…”

Section: Molecular Methodsmentioning

confidence: 99%

Molecular Phylogenetics of the Chiropteran Family Vespertilionidae

Hoofer

Bussche

2003

Acta Chiropterologica

189

168

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“…and deposited in the Mammalogy Division of Museo de Zoología at the Pontificia Universidad Católica del Ecuador, Quito, Ecuador (QCAZ 8478). Tissues (heart, kidney and liver) were preserved in lysis buffer (Longmire et al 1997) and deposited at the Angelo State Natural History Collection, Angelo State University, San Angelo, Texas, USA (ASK 7645).…”

mentioning

confidence: 99%

Mammalia, Chiroptera, Emballonuridae, Peropteryx leucoptera Peters, 1867 and Peropteryx pallidoptera Lim, Engstrom, Reid, Simmons, Voss and Fleck, 2010: distributional range extensions in Ecuador

McDonough¹,

Lim²,

Ferguson³

et al. 2010

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We reevaluate vouchered records of Peropteryx leucoptera in Ecuador with regard to the morphologically similar and newly described species, P. pallidoptera. The western-most distributional occurrence of P. pallidoptera is documented. Additionally, we describe a new record of P. leucoptera collected at Palma Roja, Cuyabeno Faunistic Reserve in Sucumbíos Province, Ecuador that represents the first record for this species in Sucumbíos Province and the northern-most distributional occurrence for Ecuador.

show abstract

Use of "Lysis Buffer" in DNA isolation and its implication for museum collections

Cited by 329 publications

References 1 publication

Mitochondrial DNA and microsatellite markers evidence a different pattern of hybridization in red-legged partridge (Alectoris rufa) populations from NW Italy

Mitochondrial DNA and microsatellite markers evidence a different pattern of hybridization in red-legged partridge (Alectoris rufa) populations from NW Italy

Molecular Phylogenetics of the Chiropteran Family Vespertilionidae

Mammalia, Chiroptera, Emballonuridae, Peropteryx leucoptera Peters, 1867 and Peropteryx pallidoptera Lim, Engstrom, Reid, Simmons, Voss and Fleck, 2010: distributional range extensions in Ecuador

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