Use of Laser fluorescence and Scanning Electron Microscope to Evaluate Remineralization of Incipient Enamel Lesions Remineralized by Topical Application of Casein Phospho Peptide Amorphous Calcium Phosphate (CPP-ACP) Containing Cream

Abstract

The aim of this in vitro study was to evaluate the remineralization of incipient enamel lesions by the topical application of Casein PhosphoPeptide-Amorphous Calcium Phosphate (CPP-ACP) using laser fluorescence and scanning electron microscope. Sixty caries free extracted teeth were used in the study. Forty teeth were used as test samples, ten as positive and ten as negative controls. The samples were demineralized and then remineralized by the topical application of CPP-ACP for a period of 14 days. The remineralization was evaluated with the use of laser fluorescence and scanning electron microscope (SEM). The results of this study showed that the laser fluorescent readings of test samples after remineralization were highly significant (p <0.001). A significant number of test samples observed under SEM showed high scores of remineralization.

In vitro evaluation of the efficacy of laser fluorescence (DIAGNOdent) to detect demineralization and remineralization of smooth enamel lesions

“…Then the blocks were immersed in a 12 ml demineralization solution made of 1% sodium carboxymethyl cellulose in 0.1 M of lactic acid (containing 3 mM of calcium and 1.8 mM of phosphate, pH = 4). [18]…”

The effect of resin infiltration on proximal caries lesions in primary and permanent teeth. A systematic review and meta-analysis of clinical trials

“…Consequently, noninvasive measures have been developed with promising results on halting the lesion progression. These measures include oral hygiene intensified protocols by mechanical removal of plaque with flossing or interdental brushing, dietary advice, chemical control of cariogenic bacterial load by in office application of chlorhexidine varnishes, or re-mineralizing treatments with in office topical fluoride or home use of casein phosphopeptide [13,14,15]. However, the effectiveness of the above measures may be compromised by poor patient compliance and recall visits treatment costs [16][17][18][19][20][21].…”

In vivo remineralising effect of GC Tooth Mousse on early dental enamel lesions: SEM analysis

“…The application of a CPPs toothpaste and sodium fluoride (Colgate Neutrafluor 9,000 ppm) (NaF) can provide significant additional prevention of enamel demineralisation when resin-modified glass ionomer cement (RMGIC) is used for bonding molar tubes for orthodontic patient as preventive actions 7 . An in vitro study to evaluate the remineralisation of incipient enamel lesions by the topical application of Casein Phosphopeptide-Amorphous Calcium Phosphate (CPP-ACP) using laser fluorescence and scanning electron microscope showed high scores of remineralisation 8 .…”