Use of ISSR, SRAP, and RAPD markers to assess genetic diversity in Turkish melons

Yıldız, Mehtap; Ekbiç, Ercan; Keleş, Davut; Şensoy, Suat; Abak, K.

doi:10.1016/j.scienta.2011.06.048

Cited by 41 publications

(32 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Eastern and South-eastern Anatolian melon landraces and reference cultivars were chosen based on the study of Yildiz et al (2011) Tab. 1.…”

Section: Plant Materialsmentioning

confidence: 99%

“…Thus, Turkish local melon genotypes have been collected for use in breeding programs, and reasonable collection of germplasm exist at Aegean Agricultural Research Institute-Izmir, Turkey and Faculty of Agriculture, University of Cukurova, Adana, Turkey (Küçük et al, 2002;Sensoy et al, 2007). Yildiz et al (2011) also stated that there is a large genetic diversity among melon genotypes in Turkey and most of the melon genotypes in Eastern and South-eastern Anatolian region have distinct groupings in the obtained dendograms. Escribano and Lazaro (2009) stated that morphological analysis is an important requirement for the initial evaluation of genetic resources and the accurate identification of local landraces.…”

Section: Introductionmentioning

confidence: 99%

“…Sensoy et al (2007) used random amplified polymorphic DNA markers (RAPD) to differentiate Turkish melon germplasm. Yildiz et al (2011) used ISSR and SRAP to assess genetic diversity of Turkish melon germplasm. More recently, microsatellite analyses (Kacar et al, 2012) have also been used to estimate genetic diversity among Turkish melon accession.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Morphological and Molecular Characterization of Turkish Landraces of Cucumis melo L.

Yıldız

Akgul

Şensoy

2014

Not Bot Hort Agrobot Cluj

Self Cite

View full text Add to dashboard Cite

Twenty-four accessions covering different groups of Cucumis melo L. from Eastern and South-eastern Anatolian regions of Turkey were characterized by using 43 morphological traits and 207 markers obtained from 31 ISSR and 16 SSR primers. The genetic relatedness was studied by examining the Euclidian/UPGMA dendrogram obtained from the combined phenotypicmolecular data. In the combined morphological-molecular dendrogram, there were two main clusters. Sweet and non-sweet melon groups were separated and the flexuosus group accessions were discriminated from the sweet ones, but the momordica group accession was clustered with the sweet ones. Unclear South-eastern Anatolian accessions were sub-clustered separately among the sweet ones. Principle component analysis (PCA) of morphological characters was used in detail to discriminate melon accessions. The cumulative proportion of variation reached 44% by first three PCA axes. The first component was mainly based on sex expression, ovary index, ovary shape, flesh thickness, seed cavity length, seed cavity width, soluble solids content, fruit shape, aroma, netting, and taste. The PCA plot based on all measured traits allowed distinction between flexuosus group, subsp. agrestis and reticulatus group. A high variation among groups was observed for the fruits characters. Netting, aroma and abscission of peduncle represent reticulatus group; a small fruit size, strong typical aroma and secondary colour distribution characterize dudaim group. Monoecy, very long fruit shape and mature fruit rind colour discriminate flexuous group; Ovary index, fruit size and flesh width distinguish subsp. agrestis group. These findings indicated wide range of variations for investigated characteristics in Turkish gene pool that provides a good source of diversity to use in melon improvement program for better yield and other traits of interest.

show abstract

“…Eastern and South-eastern Anatolian melon landraces and reference cultivars were chosen based on the study of Yildiz et al (2011) Tab. 1.…”

Section: Plant Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Morphological and Molecular Characterization of Turkish Landraces of Cucumis melo L.

Yıldız

Akgul

Şensoy

2014

Not Bot Hort Agrobot Cluj

Self Cite

View full text Add to dashboard Cite

show abstract

“…SRAP is new DNA marker invented by Li and Quiros (2001). SRAP has been used to construct genetic maps (Pan, 2005;Yu et al, 2007;Liu et al, 2011;Zhang et al, 2011) and perform genetic polymorphism research (Ferriol et al, 2004;Li et al, 2010;Wang et al, 2010;Yildiz et al, 2011). Of 121 primer combinations used in the present study for SRAP assay, 7 showed differences in SRAP patterns such as DNA fragment deletions or additions between the 6 mutants and the control plants.…”

Section: Discussionmentioning

confidence: 90%

SRAP analysis of DNA base sequence changes in lotus mutants induced by Fe+ implantation

Deng

Qin²,

Gao³

et al. 2013

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Ion implantation, a new biophysically mutagenic technique, has shown great potential for crop breeding. To reveal the mutation effect of low-energy ion implantation on Baiyangdian red lotus, sequence-related amplified polymorphism markers were used to amplify and detect the DNA sequence differences in mutants induced by Fe + ion implantation. A total of 121 primer combinations were tested in 6 mutants and a control. Seven primer combinations (me1 + em3, me1 + em14, me9 + em3, me8 + em2, me6 + em1, me11 + em5, and me6 + em5) generated clear bands with high polymorphism and good repeatability. The results showed that among 15,317 bases cloned, 146 bases in 6 mutants were different from those of the wild type, showing a variation frequency of 0.95%. The types of base changes included deletion, insertion, transversion, and transition. Adenine was more sensitive to the irradiation than were the other bases. The results suggested that mutational "hotspots" probably exists in lotus and are induced by low-energy ion implantation.

show abstract

“…The gene diversity obtained with RAPD markers among different C. pepo landraces from northern KwaZulu-Natal was less than that obtained with AFLP markers among different C. moschata accessions (Wu et al, 2011); with RAPD among Trichosanthes dioica accessions (Khan et al, 2009); and also with inter-simple sequence repeat (ISSR), SRAP and RAPD markers among Turkish and Foreign Cucumis melo genotypes (Yildiz et al, 2011). The production of a specific RAPD marker by primer CB23 (1000 bp) in both MNS and MS populations from Umkhanyakude district indicated the effect of eco-geographic differences in gene diversity of plants, where landraces from the same geographic area are closely related (Ferriol et al, 2004a,b;Du et al, 2011).…”

Section: Polymorphism In C Pepo Populationsmentioning

confidence: 99%

Genetic diversity in Cucurbita pepo landraces from northern KwaZulu-Natal, South Africa, revealed by random amplified polymorphic DNA (RAPD) markers

Ntuli¹,

Zobolo²,

Tongoona³

et al. 2013

Afr. J. Biotechnol.

View full text Add to dashboard Cite

show abstract

Use of ISSR, SRAP, and RAPD markers to assess genetic diversity in Turkish melons

Cited by 41 publications

References 14 publications

Morphological and Molecular Characterization of Turkish Landraces of <i>Cucumis melo</i> L.</span>

Morphological and Molecular Characterization of Turkish Landraces of <i>Cucumis melo</i> L.</span>

SRAP analysis of DNA base sequence changes in lotus mutants induced by Fe+ implantation

Genetic diversity in Cucurbita pepo landraces from northern KwaZulu-Natal, South Africa, revealed by random amplified polymorphic DNA (RAPD) markers

Contact Info

Product

Resources

About