Use of inter simple sequence repeats and protein markers in assessing genetic diversity and relationships among four rabbit genotypes

El-Sabrout, Karim; Aggag, Sarah

doi:10.4995/wrs.2015.3912

Cited by 11 publications

(6 citation statements)

References 10 publications

(8 reference statements)

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“…Rabbits are good potential animal protein source for human (El-Sabrout & Aggag, 2015;Soliman and El-Sabrout, 2020). A rabbit doe (female) can be expected to produce more than 50 weaned young per year, representing a total of 120 kg rabbit meat at market age.…”

Section: Introductionmentioning

confidence: 99%

Improving the reproductive, immunity and health status of rabbit does using honey bee venom

Elkomy

El-Hanoun

Abdella

et al. 2021

Animal Physiology Nutrition

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The aim of the present study was to investigate the long-term effect of using bee venom (BV) on the reproductive performance, immune, and health status of rabbit does and its effect on their litters. Sixty mature does, from Spanish V-line rabbit stock, were randomly assigned to four homogeneous groups with 15 does each. The 1 st , 2 nd and 3 rd groups were injected twice weekly under the neck skin with 0.1 ml solution contains 0.1, 0.2 and 0.3 mg BV/rabbit respectively. The 4 th group served as a control group. From the results, litter size at birth, litter weight and survival rate at weaning age as well as milk yield were significantly (p ≤ 0.05) increased in BV groups than in the control group. Serum estradiol 17β (E2) was significantly (p ≤ 0.05) higher (15%) in the rabbit does treated with BV compared to the control group. The treated does with BV at any study doses showed a gradual and significant (p ≤ 0.05) decrease (12%) in serum progesterone levels (P4) compared to the control. They also showed a significant (p≤0.05) increase in conception (17%) and fertility rates (10%) compared to the control does. Treatment of rabbit does with BV caused a gradual and significant (p ≤ 0.05) reduction in both aspartate aminotransferase (AST) (16%) and alanine aminotransferase (ALT) (37%) liver enzyme activities. Additionally, results have shown that BV resulted in a gradual and significant (p ≤ 0.05) increase in total antioxidant capacity (TAC), antioxidative enzymes such as glutathione S-transferase (GST) and glutathione peroxidase (GPx), serum IgG, IgM and IgA levels with significant (p ≤ 0.05) decrease in malondialdehyde (MDA) and thiobarbituric acid reactive substances (TBARS) in BV groups compared to the control group. Results suggest that BV can be used in rabbit farming as an effective and safe alternative to artificial chemical drugs (sexualstimulants) to improve certain reproductive traits, immune response and health.

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Section: Introductionmentioning

confidence: 99%

Improving the reproductive, immunity and health status of rabbit does using honey bee venom

Elkomy

El-Hanoun

Abdella

et al. 2021

Animal Physiology Nutrition

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“…Rabbit has its importance as supplier of meat and it has become one of the necessary sources of food in some country. Molecular techniques in rabbit’s field become very important tools for the detection of markers linked to economical traits [ 1 ]. In breeding programs, data utility from associate genes has potential to basically improve the accuracy of selection [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Associations between single nucleotide polymorphisms in multiple candidate genes and body weight in rabbits

El-Sabrout¹,

Aggag²

2017

Vet World

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Aim:In this study, we examined parts of six growth genes (growth hormone [GH], melanocortin 4 receptor [MC4R], growth hormone receptor [GHR], phosphorglycerate mutase [PGAM], myostatin [MSTN], and fibroblast growth factor [FGF]) as specific primers for two rabbit lines (V-line, Alexandria) using nucleotide sequence analysis, to investigate association between detecting single nucleotide polymorphism (SNP) of these genes and body weight (BW) at market.Materials and Methods:Each line kits were grouped into high and low weight rabbits to identify DNA markers useful for association studies with high BW. DNA from blood samples of each group was extracted to amplify the six growth genes. SNP technique was used to study the associate polymorphism in the six growth genes and marketing BW (at 63 days) in the two rabbit lines. The purified polymerase chain reaction products were sequenced in those had the highest and lowest BW in each line.Results:Alignment of sequence data from each group revealed the following SNPs: At nucleotide 23 (A-C) and nucleotide 35 (T-G) in MC4R gene (sense mutation) of Alexandria and V-line high BW. Furthermore, we detected the following SNPs variation between the two lines: A SNP (T-C) at nucleotide 27 was identified by MC4R gene (sense mutation) and another one (A-C) at nucleotide 14 was identified by GHR gene (nonsense mutation) of Alexandria line. The results of individual BW at market (63 days) indicated that Alexandria rabbits had significantly higher BW compared with V-line rabbits. MC4R polymorphism showed significant association with high BW in rabbits.Conclusion:The results of polymorphism demonstrate the possibility to detect an association between BW in rabbits and the efficiency of the used primers to predict through the genetic specificity using the SNP of MC4R.

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“…Therefore, studying the milk protein polymorphism is very importance in animal breeding [ 5 ]. Protein polymorphism provides beneficial information such as the relationships between rabbit genotypes and their biochemical expressions [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Comparison of milk production and milk composition for an exotic and a local synthetic rabbit lines

2017

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Aim:Basic objective of this research was to compare two rabbit lines: V-line (exotic line), Alexandria (local synthetic line) for yield and composition of milk product and their effect on productive performance of rabbits.Materials and Methods:The experiment was conducted on 80 does and their kits. Milk yield (MY) of each doe and milk composition (MC) were recorded at 3rd week of lactation.Results:The results of MY showed insignificant difference between V-line and Alexandria line. While the results of MC indicated significant differences in milk protein and fat between the two lines. The protein electrophoresis was used for assaying variation in milk proteins between the two lines. The banding protein patterns showed seven protein bands for Alexandria line and six bands for V line. The results demonstrated one specific protein marker at 48 KDa (κ-casein) in Alexandria doe’s milk. Moreover, the results of individual body weight at weaning age indicated that Alexandria rabbits had significantly higher body weight compared with V-line rabbits (845.33 g, 664.05 g, respectively). Alexandria line had significantly lower mortality rate compared with V-line (1.5%, 2.7%, respectively).Conclusion:The differences which obtained in Alexandria line milk may play an important role in the productive performance of rabbits.

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Use of inter simple sequence repeats and protein markers in assessing genetic diversity and relationships among four rabbit genotypes

Cited by 11 publications

References 10 publications

Improving the reproductive, immunity and health status of rabbit does using honey bee venom

Improving the reproductive, immunity and health status of rabbit does using honey bee venom

Associations between single nucleotide polymorphisms in multiple candidate genes and body weight in rabbits

Comparison of milk production and milk composition for an exotic and a local synthetic rabbit lines

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