USE OF INFORMATION THEORY AND NUMERICAL TAXONOMY METHODS FOR EVALUATING THE QUALITY OF THIN-LAYER CHROMATOGRAPHIC SEPARATIONS OF FLAVONOIDS AND PHENOLIC ACIDS OF RHAMNI CATHARTICI FRUCTUS

Abstract

ORDER REPRINTS 84MEDIj-ARIj ET AL. ABSTRACTA rational selection of a restricted set from fifteen available chromatographic systems for the separation of flavonoids and phenolic acids identified in the methanolic extract of Rhamni cathartici fructus is discussed. Series of mathematical techniques for the evaluation of solvents and solvent combinations in thinlayer chromatography of flavonoids and phenolic acids have been investigated. The chromatographic systems are classified according to their mutual resemblance by numerical taxonomy techniques. The selection criterion in the groups, obtained by numerical taxonomy classification, is the information content or discriminating power. The numerical taxonomic and information theoretical selection procedures are compared and their respective advantages and disadvantages discussed.

Simultaneous Identification and Quantification of the Sugar, Sugar Alcohol, and Carboxylic Acid Contents of Sour Cherry, Apple, and Ber Fruits, as Their Trimethylsilyl Derivatives, by Gas Chromatography−Mass Spectrometry

“…To our knowledge, there is no literature data on the acid and sugar/sugar alcohol contents of the ber fruits. However, several papers were found studying the triterpenoid of Ziziphus xylopyrus (), the lectin () and cyclopeptide alkaloid () of Z. mauritiana , the flavonoids ( − ), the antisweet substances (), the fatty acid composition of its lipid fraction (), and the multipurpose utility ( , ) of Ziziphus jujuba .…”

Optimization

“…For evaluation of the separation power of a chromatographic system or for the rational selection of the mobile-phase system, the information quantity or the discriminating power is used as the selection criterion in the groups obtained by numerical taxonomy (66,67). Another rational and logical choice of the optimum solvent system can be accomplished by using the information quantity and objective function (Eq.…”