Simultaneous Identification and Quantification of the Sugar, Sugar Alcohol, and Carboxylic Acid Contents of Sour Cherry, Apple, and Ber Fruits, as Their Trimethylsilyl Derivatives, by Gas Chromatography−Mass Spectrometry

Abstract

Our gas chromatography-mass spectrometry method--developed for the simultaneous quantitation of mono-, di-, and trisaccharides, sugar alcohols, caboxylic and amino acids, measured as their trimethylsilyl-(oxime) ether/ester derivatives, from one solution by a single injection, prepared in the presence of the fruit matrix--has been extended/utilized for special purposes. The compositions of (i) freshly harvested and stored sour cherries (Prunus cerasus), (ii) apples obtained from organic and integrated productions (Malus domestica), and (iii) green and ripe bers (Zizyphus mauritiana L.) were compared. On the basis of earlier, basic researches (derivatization, quantitation, and fragmentation studies of authentic compounds), we demonstrate the reproducible quantitation of the main and minor constituents in a wide concentration range (approximately 1 x 10(-)(3) to >/=40%, in total up to < or =98%, calculated on dry matter basis of the fruit matrices). Reproducibility of quantitations, calculated on the basis of their total ion current values, provided an average reproducibility of 3.3 (sour cherries), 6.2 (apple), and 4.3 (ber) RSD %, respectively.

Biochemical, sensorial and genomic profiling of traditional Italian tomato varieties

“…500 l of Wltered sample were dried and dissolved in 500 l of borate buVer (0.1 M, pH 10.4). The solution was mixed with Fmoc reagent (500 l, 5.8 mM in acetone) (Gartenmann and Kochhar 1999). The mixture was extracted twice with 2 ml of exane/ethyl acetate (80:20).…”

Comprehensive two-dimensional gas chromatography, retention indices and time-of-flight mass spectra of flavonoids and chalcones

“…The successful separation of TMS derivatives of flavonoids by GC followed by their mass spectrometric identification (GC-MS) has been reported by several researchers [19][20][21]. The ease of preparation and their excellent GC properties has led to an increased use of trimethylsilyl (TMS) derivatives for polar molecules for many compounds [22][23][24][25][26].…”

Sorbitol, Rubus fruit, and misconception

“…Additionally, the sugar alcohol concentration within the edible parts of a plant or fungus fluctuate due to many variables, including fraction (leaf, stem, fruit, etc. ), ripeness, species, genus, cultivar, genotype, environment, cultivation, processing, and storage conditions (Cantin et al, 2009;Durst et al, 1995;Fuzfai, Katona, Kovacs, & Molnar-Perl, 2004;Hecke et al, 2006;Liu et al, 1999;Makinen & Soderling, 1980;Merchant & Richter, 2011;Moing, 2000;Wallaart, 1980;Wodner et al, 1988).…”

“…Although, some references have not reported every individual sugar listed above and discrepancies might have arisen from variation in sample, preparation, column, detector, or method conditions (Ellefson, 2005;Fuzfai et al, 2004;Muir et al, 2009). Table 1 summarises total sugars and total sugar alcohols in fresh weight (fw) fruit from 22 genera and species, by dry weight (dw) in Table 2, and by their products in Table 3.…”