Use of Impedance Spectroscopy to Assess the Effect of Laminins on the in vitro Differentiation of Intestinal Cells

Knyazev, E N; Maltseva, D. V.; Sakharov, D. A.; Gerasimenko, T. N.

doi:10.21519/0234-2758-2019-35-6-102-107

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…The human adenocarcinoma cell line Caco-2 is successfully used as a model of the human intestinal barrier. During long-term cultivation, Caco-2 cells polarize, form microvilli, tight junctions, extracellular matrix, and begin to express specific enterocyte markers (146)(147)(148). Models based on Caco-2 cells using microfluidic devices that create medium flow make the model even closer to the physiological conditions of human intestine (149)(150)(151).…”

Section: Using Tissue Barrier Models To Study Coronavirus Transcytosismentioning

confidence: 99%

Endocytosis and Transcytosis of SARS-CoV-2 Across the Intestinal Epithelium and Other Tissue Barriers

2021

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Since 2003, the world has been confronted with three new betacoronaviruses that cause human respiratory infections: SARS-CoV, which causes severe acute respiratory syndrome (SARS), MERS-CoV, which causes Middle East respiratory syndrome (MERS), and SARS-CoV-2, which causes Coronavirus Disease 2019 (COVID-19). The mechanisms of coronavirus transmission and dissemination in the human body determine the diagnostic and therapeutic strategies. An important problem is the possibility that viral particles overcome tissue barriers such as the intestine, respiratory tract, blood-brain barrier, and placenta. In this work, we will 1) consider the issue of endocytosis and the possibility of transcytosis and paracellular trafficking of coronaviruses across tissue barriers with an emphasis on the intestinal epithelium; 2) discuss the possibility of antibody-mediated transcytosis of opsonized viruses due to complexes of immunoglobulins with their receptors; 3) assess the possibility of the virus transfer into extracellular vesicles during intracellular transport; and 4) describe the clinical significance of these processes. Models of the intestinal epithelium and other barrier tissues for in vitro transcytosis studies will also be briefly characterized.

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Section: Using Tissue Barrier Models To Study Coronavirus Transcytosismentioning

confidence: 99%

Endocytosis and Transcytosis of SARS-CoV-2 Across the Intestinal Epithelium and Other Tissue Barriers

2021

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“…Differentiated Caco-2 (dCaco-2) cells acquire enterocyte properties, such as cylindrical cell shape, formation of intercellular junctions and microvilli, specific enzyme and marker expression ( Ding et al, 2021 ). Extracellular matrix can support cell attachment, proliferation, and differentiation ( Mutsenko et al, 2017 ), and use of laminins specific for the intestinal basal membrane can bring the gut model properties closer to conditions in vivo ( Nikulin et al, 2018a , 2019 ). Microfluidic devices with a culture medium flow that simulates blood flow create even more physiological conditions for the cell model ( Samatov et al, 2016 , 2017 ; Sakharov et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

HIF-Dependent NFATC1 Activation Upregulates ITGA5 and PLAUR in Intestinal Epithelium in Inflammatory Bowel Disease

et al. 2021

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Intestinal epithelial cells exist in physiological hypoxia, leading to hypoxia-inducible factor (HIF) activation and supporting barrier function and cell metabolism of the intestinal epithelium. In contrast, pathological hypoxia is a common feature of some chronic disorders, including inflammatory bowel disease (IBD). This work was aimed at studying HIF-associated changes in the intestinal epithelium in IBD. In the first step, a list of genes responding to chemical activation of hypoxia was obtained in an in vitro intestinal cell model with RNA sequencing. Cobalt (II) chloride and oxyquinoline treatment of both undifferentiated and differentiated Caco-2 cells activate the HIF-signaling pathway according to gene set enrichment analysis. The core gene set responding to chemical hypoxia stimulation in the intestinal model included 115 upregulated and 69 downregulated genes. Of this set, protein product was detected for 32 genes, and fold changes in proteome and RNA sequencing significantly correlate. Analysis of publicly available RNA sequencing set of the intestinal epithelial cells of patients with IBD confirmed HIF-1 signaling pathway activation in sigmoid colon of patients with ulcerative colitis and terminal ileum of patients with Crohn’s disease. Of the core gene set from the gut hypoxia model, expression activation of ITGA5 and PLAUR genes encoding integrin α5 and urokinase-type plasminogen activator receptor (uPAR) was detected in IBD specimens. The interaction of these molecules can activate cell migration and regenerative processes in the epithelium. Transcription factor analysis with the previously developed miRGTF tool revealed the possible role of HIF1A and NFATC1 in the regulation of ITGA5 and PLAUR gene expression. Detected genes can serve as markers of IBD progression and intestinal hypoxia.

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Use of Impedance Spectroscopy to Assess the Effect of Laminins on the in vitro Differentiation of Intestinal Cells

Cited by 2 publications

References 17 publications

Endocytosis and Transcytosis of SARS-CoV-2 Across the Intestinal Epithelium and Other Tissue Barriers

Endocytosis and Transcytosis of SARS-CoV-2 Across the Intestinal Epithelium and Other Tissue Barriers

HIF-Dependent NFATC1 Activation Upregulates ITGA5 and PLAUR in Intestinal Epithelium in Inflammatory Bowel Disease

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