Use ofBacillus brevisfor Synthesis and Secretion of Des-B30 Single-Chain Human Insulin Precursor

Modern Status and Perspectives of Bacillus Bacteria as Probiotics

“…Recombinant B. subtilis strain (Subalin) is approved as a drug by the national authority of Ukraine. B. brevis strain was transformed with the plasmid, carrying a synthetic gene of a single chain human insulin precursor [96]. Authors demonstrated that recombinant product, obtained from the culture supernatant, had the biological activity, similar to human insulin.…”

Recombinant Probiotics: Future Perspectives in Disease Treatment

“…Bacillus strains can be also engineered for extracellular production of recombinant proteins because these bacteria have an intrinsic characteristic to secrete different proteins. B. brevis strain was transformed with the plasmid, carrying a synthetic gene of a single chain human insulin precursor [52]. Biological activity of the recombinant product, obtained from the culture supernatant was similar to human insulin.…”