Use of high concentrations of dimethyl sulfoxide for cryopreservation of HepG2 cells adhered to glass and polydimethylsiloxane matrices

Nagahara, Yukitoshi; Sekine, Hiroaki; Otaki, Mari; Hayashi, Mitsuru; Murase, Norio

doi:10.1016/j.cryobiol.2015.11.004

Cited by 4 publications

(3 citation statements)

References 29 publications

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“…Konsentrasi optimal DMSO sangat bervariasi tergantung pada tipe sel yang dikriopreservasi, jenis pengencer, suhu dan waktu pemaparan. Konsentrasi DMSO yang optimal untuk preservasi mesenchymal stem cells adalah 5% (Ock dan Rho, 2011), pada HepG2 cells adalah 20% (Nagahara et al, 2016). Peningkatan konsentrasi DMSO dari 5% ke 30%, lama pemaparan dari 10 ke 30 menit, dan suhu pemaparan dari dari 4 o C ke 37 o C menyebabkan penurunan viabilitas sel fibroblas kulit (Wang et al., 2007).…”

Section: Rancangan Percobaan Dan Analisis Dataunclassified

Kualitas Sperma Beku Sapi Bali dalam Pengencer Air Kelapa Modifikasi dengan Berbagai Aras Dimethyl Sulfoxide (FROZEN SPERM QUALITY OF BALI BULLS IN MODIFIED COCONUT WATER EXTENDER WITH DIFFERENT DIMETHYL SULFOXIDE CONCENTRATION)

Hine

Uly

Nalley

et al. 2019

JVet

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Dimethyl Sulfoxide (DMSO) is one type of cryoprotectant which has a low molecular weight so that it is easier to enter cells when cryopreservation. The purpose of this study was to explore the optimal concentration of DMSO in modified coconut water (mCW) extender that were able to maintain frozen sperm quality of bali bulls. Semen was collected from two four-year old bali bulls by artificial vagina. Good quality semen diluted with mCW (young coconut water + 20% egg yolk + 7.5 % moringa leaf extract) and supplemented by 3, 5, or 7% DMSO. Semen was filled into 0.25 ml ministraw, and was incubated in a refrigerator at 5°C for four hours, frozen on the surface of liquid nitrogen for 10 minutes and then dipped into liquid nitrogen. The quality of post thawing sperm was measured 24 hours later by placing the ministraw of frozen semen into water at 37oC for 30 seconds. Data were analyzed by analysis of variance and continued with Duncan test. Postthawing observations showed that bali bulls sperm cryopreserved at 3% DMSO yielded higher motility and viability (p<0.05) i.e. 36 and 44.15%, than DMSO 5% i.e. 18 and 23.65%, and DMSO 7% i.e. 7 and 12.62%. The recovery rate of sperm cryopreserved at 3% DMSO was also higher (p<0.05) than DMSO 5 and 7%, successively 45.65, 23.06, and 8.86%. The results of this study concluded that the optimal concentration of DMSO in mCW diluent to maintain frozen sperm quality of bali bulls was 3%.

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Section: Rancangan Percobaan Dan Analisis Dataunclassified

Kualitas Sperma Beku Sapi Bali dalam Pengencer Air Kelapa Modifikasi dengan Berbagai Aras Dimethyl Sulfoxide (FROZEN SPERM QUALITY OF BALI BULLS IN MODIFIED COCONUT WATER EXTENDER WITH DIFFERENT DIMETHYL SULFOXIDE CONCENTRATION)

Hine

Uly

Nalley

et al. 2019

JVet

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“…21 A study by Suhodolcǎn et al employed glycerol in the cryopreservation of allogeneic patellar tendon grafts and found that 86% of the ultimate stiffness level was maintained after 1 month of cryopreservation. 20 However, DMSO is toxic that affects DNA structure and causes protein denaturation, 22 while the main concern associated with glycerol is its prolonged unloading time post-thawed and inability to achieve complete elution. 23 There is an urgent need to explore alternative CPAs for the long-term cryopreservation of tendons.…”

Section: ■ Introductionmentioning

confidence: 99%

Plunge-Freezing Cryopreservation of Tendons

Li,

Liu,

Zhang

et al. 2024

Langmuir

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Allograft transplantation is an important method for tendon reconstruction after injury, and its clinical success highly relies on the storage and transportation of the grafts. Cryopreservation is a promising strategy for tendon storage. In this study, we report a novel cryopreservation agent (CPA) formulation with a high biocompatibility for tendon cryopreservation. Mainly composed of natural zwitterionic betaine and the biocompatible polymer poly(vinylpyrrolidone) (PVP), it exhibited ideal abilities to depress the freezing point and inhibit ice growth and recrystallization. Notably, after cryopreservation via plungefreezing for 1 month, Young's modulus (144 MPa, 98% of fresh tendons) and ultimate stress (46.7 MPa, 99% of fresh tendons) remained stable, and the cross-linking of collagen microfibers, protein structures, and glycosaminoglycan (GAG) contents changed slightly. These results indicate that the formulation (5 wt % betaine and 5 wt % PVP in phosphate-buffered saline, PBS solution) effectively maintains the biomechanical properties and tissue structure. This work offers a novel cryopreservation method for tendons and may also provide insights into the long-term preservation of various other tissues.

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“…16,17 Notably, the use of high concentrations of the cryoprotectant DMSO, which enhances the dehydration effect and inhibits of icecrystal formation, was reported to allow direct cryopreservation of adherent cells cultured on polydimethylsiloxane substrate. 18 However, the development of a systematic approach for the cryopreservation of adherent cells remains under investigation.…”

Section: Introductionmentioning

confidence: 99%

Direct cryopreservation of adherent cells on an elastic nanofiber sheet featuring a low glass-transition temperature

2017

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Use of high concentrations of dimethyl sulfoxide for cryopreservation of HepG2 cells adhered to glass and polydimethylsiloxane matrices

Cited by 4 publications

References 29 publications

Kualitas Sperma Beku Sapi Bali dalam Pengencer Air Kelapa Modifikasi dengan Berbagai Aras Dimethyl Sulfoxide (FROZEN SPERM QUALITY OF BALI BULLS IN MODIFIED COCONUT WATER EXTENDER WITH DIFFERENT DIMETHYL SULFOXIDE CONCENTRATION)

Kualitas Sperma Beku Sapi Bali dalam Pengencer Air Kelapa Modifikasi dengan Berbagai Aras Dimethyl Sulfoxide (FROZEN SPERM QUALITY OF BALI BULLS IN MODIFIED COCONUT WATER EXTENDER WITH DIFFERENT DIMETHYL SULFOXIDE CONCENTRATION)

Plunge-Freezing Cryopreservation of Tendons

Direct cryopreservation of adherent cells on an elastic nanofiber sheet featuring a low glass-transition temperature

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