Use of Gas Chromatography for Determining Catabolic Products of Arginine by Bacteria

Moss, C W; Lambert, M A; Cherry, William B.

doi:10.1128/aem.23.5.889-893.1972

Cited by 3 publications

(2 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the induction medium and the three test media described above are required for the simultaneous detection of the dihydrolase and decarboxylase systems in microorganisms. The results listed in Table 1 agree with those obtained by identifying the end products of the arginine dihydrolase enzymes and decarboxylases of arginine, lysine, and ornithine with one-dimensional TLC on cellulose plates (10,11,21,29,31,32) and with gas-liquid chromatography (15,19).…”

Section: Discussionsupporting

confidence: 81%

“…Moreover, a suitable solvent system has not been reported for the separation of all possible components (arginine, citrulline, omithine, putrescine, and agmatine) in the test system (31). Gas-liquid chromatography has also been used for demonstrating ornithine and lysine decarboxylase activities (15) and for detecting arginine metabolites (19). However, the gas-liquid chromatography procedures described were not shown to be capable of detecting the decarboxylases of glutamic acid, histidine, phenylalanine, tryptophan, or tyrosine, and they were less efficient for testing a large number of specimens.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Rapid method for simultaneous detection of the arginine dihydrolase system and amino acid decarboxylases in microorganisms

Chen¹,

Culbertson²,

Knapp³

et al. 1982

J Clin Microbiol

View full text Add to dashboard Cite

A specific procedure has been developed for the detection of the first two enzymes involved in the arginine dihydrolase system and the detection of the decarboxylases of arginine, glutamic acid, histidine, lysine, ornithine, phenylalanine, tryptophan, and tyrosine. A loopful of growth of each organism from dihydrolase-decarboxylase induction agar medium (or broth) was washed and incubated separately with 0.2-ml samples of three test media supplemented with different amino acids. Each spent test medium was dansylated, and the dansyl derivatives were separated by two-dimensional thin-layer chromatography on polyamide sheets. The end products (citrulline, ornithine, y-amino-n-butyric acid, and amines) produced during incubation were estimated by comparing the fluorescent intensities of end products from the spent test media and of the corresponding parent amino acids from test medium controls after thin-layer chromatography. The method is reproducible, requiring incubation of an organism in three test media for 1 h for simultaneous detection of the first two enzymes involved in the arginine dihydrolase system and of eight amino acid decarboxylases. This method has been successfully applied to gram-positive and gramnegative microorganisms and also to Mycoplasmatales. It could simplify and improve the accuracy of the corresponding biochemical tests performed in clinical laboratories for the identification and differentiation of microorganisms, and it may prove particularly useful for the differentiation of species of Pseudomonas and Mycoplasma. Enzymes involved in the arginine dihydrolase system and the amino acid decarboxylases are widely distributed in microorganisms (1, 2, 8). The microbial arginine dihydrolase system which converts arginine to ornithine via citrulline consists of three enzyme reactions: arginine + H20-* citrulline + NH3 by arginine deiminase; citrulline + Pi ornithine + carbamyl

show abstract

Section: Discussionsupporting

confidence: 81%

mentioning

confidence: 99%

Rapid method for simultaneous detection of the arginine dihydrolase system and amino acid decarboxylases in microorganisms

Chen¹,

Culbertson²,

Knapp³

et al. 1982

J Clin Microbiol

View full text Add to dashboard Cite

show abstract

Chapter III. Gas-Liquid Chromatographic Chemotaxonomy

Drucker

1976

Methods in Microbiology

View full text Add to dashboard Cite

Bacterial sources of putrescine and cadaverine in chill stored vacuum‐packaged beef

Dainty

Edwards

Hibbard

et al. 1986

Journal of Applied Bacteriology

View full text Add to dashboard Cite

Of the meat strains of streptobacteria, leuconostocs, Enterobacteriaceae and Brochothrix thermosphacta tested, only Hafnia alvei and Serratia liquefaciens showed diamine-producing potential during growth in pure culture on beef stored in vacuum packs at 1 degree C. Both organisms produced cadaverine at concentrations similar to those reported previously in naturally contaminated beef stored under the same conditions. Putrescine concentrations produced by the two organisms, however, were an order of magnitude lower. During the growth on beef of either H. alvei or S. liquefaciens in mixed culture with arginine-utilizing strains of streptobacteria, putrescine as well as cadaverine concentrations were similar to those detected in naturally contaminated samples.

show abstract

Use of Gas Chromatography for Determining Catabolic Products of Arginine by Bacteria

Cited by 3 publications

References 8 publications

Rapid method for simultaneous detection of the arginine dihydrolase system and amino acid decarboxylases in microorganisms

Rapid method for simultaneous detection of the arginine dihydrolase system and amino acid decarboxylases in microorganisms

Chapter III. Gas-Liquid Chromatographic Chemotaxonomy

Bacterial sources of putrescine and cadaverine in chill stored vacuum‐packaged beef

Contact Info

Product

Resources

About