Use of FTA Cards for Direct Sampling of Patients' Lesions in the Ecological Study of Cutaneous Leishmaniasis

Abstract

The FTA card (Whatman) was assessed for its utility as a molecular epidemiological tool in collecting samples from patients with leishmaniasis in Peru because the card has a variety of merits; it is less invasive for patients and easy to handle for both physicians and other medical personnel for sample collection or diagnosis, in addition to its simplicity and easy countrywide and/or intercountry transportation for analysis.

Natural Leishmania Infection of Lutzomyia auraensis in Madre de Dios, Peru, Detected by a Fluorescence Resonance Energy Transfer–Based Real-Time Polymerase Chain Reaction

“…For example, L. (V.) shawi was reported in the same region of Peru where our study was conducted. 17 Also, a study in Venezuela detected Leishmania (L.) venezuelensis in Lu. trinidadensis, the same sand fly species found to be Leishmania positive in our study but without an identifiable species.…”

“…3,[15][16][17] Capture of sand flies and identification. Peridomiciliary sand fly specimens were captured in September, November, and December of 2009 (before and during the rainy season) in the vicinity of 10 arbitrarily selected houses during 10 consecutive days each month (Figure 1).…”

First human cases of Leishmania (Viannia) naiffi infection in Ecuador and identification of its suspected vector species

“…Primers used for nested PCR were L.cyt-AS (5'-GCGGAGAGRARGAAAAGGC-3') and L.cyt-AR (5'-CCACTCATAAATATACTATA-3') for the first PCR, and L.cyt-S and L.cyt-R for the second PCR (Kato et al, 2010b(Kato et al, , 2011b.…”

Leishmania species identification using FTA card sampling directly from patients’ cutaneous lesions in the state of Lara, Venezuela

“…[5][6][7] Recently, to facilitate the sample collection and DNA extraction processes, a FTA card, a filter paper that readily lyses the spotted materials and fixes nucleic acids, was used for direct sampling of patients' materials in an epidemiological study of leishmaniasis. 8 Cutaneous leishmaniasis (CL) is endemic in the state of Lara located in western 4 Venezuela, and the causative parasites have been identified as Leishmania (Viannia) braziliensis and Leishmania (Leishmania) venezuelensis. [9][10][11][12] L. (L.) venezuelensis was originally described on the basis of distinguishable MLEE patterns as a species independent of other members of the L. mexicana complex 9,10 .…”