Use of FTA card for dry collection, transportation and storage of cervical cell specimen to detect high-risk HPV

Gustavsson, Inger; Lindell, Monica; Wilander, Erik; Strand, Anders; Gyllensten, Ulf

doi:10.1016/j.jcv.2009.06.021

Cited by 74 publications

(86 citation statements)

References 23 publications

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“…Our results are consistent with those from smaller studies (n Ͻ 100) conducted in Nordic countries that observed a high overall agreement for PCR HPV detection and genotyping between cervical cells collected using a liquid-based medium and specimens collected using the FTA cartridges both among self-collected and clinician-collected samples (5,9,10,20).…”

Section: Discussionsupporting

confidence: 91%

“…Therefore, we were unable to directly evaluate the possibility that the higher prevalence of HPV detected using the FTA method is partially explained by collection order. However, Gustavson et al followed the same procedure (i.e., FTA first and dry-frozen cytobrush second) and showed that the number of copies of human single copy gene (housekeeping gene-HMBS) was much higher for the cytobrush sample than for the FTA cards (9), and in our study we did not observe evidence for reduced cytology specimen adequacy, which is consistent with there being sufficient material in the liquid-based sample. Therefore, it is unlikely that the order would be the explanation for the increased prevalence observed, but direct quantification of the DNA present in both samples (which was not conducted in the present study) would be needed to confirm this.…”

Section: Discussionsupporting

confidence: 82%

“…These researchers also found 93% agreement between FTA self-collected and liquid-based physician-collected samples among 45 women aged 23 to 51 years attending a gynecology clinic (20). Gustavsson et al evaluated cervical samples from 50 women visiting a dermatology and venereology clinic, from whom physician-collected samples were obtained using a cytobrush; each sample was first applied to FTA and then frozen dry, and the agreement was 94% (9).…”

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confidence: 99%

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Evaluation of the FTA Carrier Device for Human Papillomavirus Testing in Developing Countries

et al. 2012

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e Liquid-based methods for the collection, transportation, and storage of cervical cells are cumbersome and expensive and involve laborious DNA extraction. An FTA cartridge is a solid carrier device, easier to handle and allowing simple DNA elution for human papillomavirus (HPV) testing. HPV-DNA results from cervical specimens collected in PreservCyt medium (Hologic, Inc.) and the indicating FTA elute cartridge were compared in an area where transportation and storage may affect the performance of the test. Cervical cells from 319 young adult women enrolled in the Costa Rica Vaccine Trial were collected by a nurse using a Cervex brush (Roberts), which was placed on the FTA cartridge and subsequently rinsed in 20 ml of PreservCyt medium. Two 0.5-ml PreservCyt aliquots were frozen for HPV-PCR testing; the FTA cartridges were kept at room temperature. HPV-DNA detection and typing was performed using SPF 10 PCR/DEIA (DNA enzyme immunoassay detection of amplimers)/LiPA 25 system. The percent agreement, agreement among positives, and kappas were estimated. Positivity was higher for FTA compared to PreservCyt specimens (54.5% versus 45.8%, P < 0.001). For oncogenic types, the overall agreement was 0.92, the agreement between positives was 0.74, and the kappa was 0.79. For individual HPV types, the overall agreement ranged from 0.97 to 1.00. We did not observe reduced cytology adequacy when specimen collection for cytology was preceded by FTA collection for HPV testing. HPV-DNA detection from FTA cartridges is broadly comparable to detection from PC medium. The higher HPV detection observed for FTA-collected specimens should be explored further. FTA cartridges could provide a simpler and more cost-effective method for cervical cell collection, storage, and transportation for HPV-DNA detection in research settings in developing countries. P ersistent infection with one of approximately 13 carcinogenic human papillomaviruses (HPVs) is a necessary cause for the development of cervical cancer (14, 23). Detection of some or all of these HPV types has been shown to be useful for cervical cancer screening (1,15,(26)(27)(28).In clinical trials (including evaluation of current and future HPV vaccines) and epidemiological studies, cervical cells for HPV DNA detection are usually collected and preserved in liquid-based transport medium, which in some cases is used also for cytology slide preparation, allowing the collection of only one sample for both tests (7,12,13,24,25).However, these medium samples can be flammable and require stable transport and storage temperatures, which are difficult and expensive to provide in developing and tropical countries. If samples are tested in a different country, as is usually the case for large research studies conducted in developing countries, the exportation of such samples must fulfill international regulations regarding the transport of hazardous samples (i.e., according to the 53rd edition of IATA's DGR, specimens collected in PreservCyt [Hologic, Inc.] Moreover, these liquid-based sam...

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 82%

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confidence: 99%

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Evaluation of the FTA Carrier Device for Human Papillomavirus Testing in Developing Countries

et al. 2012

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“…Recently, the FTA card has been actively explored in cervical cancer screening as an alternative for the current liquid-based sample carrier (Gustavsson et al, 2009;Lenselink et al, 2009;Gustavsson et al, 2011). It was promising that good agreement between liquid-based sample carrier and the solid FTA card were found for several PCR-based HPV testing methods, such as GP5+/6+ and SPF (10) PCR/ DEIA/LiPA (25) (Gonzalez et al, 2012;Guan et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Clinical Evaluation of Human Papillomavirus Detection by careHPV^TMTest on Physician-Samples and Self-Samples using The Indicating FTA Elute® Card

Wang

Hu²,

Chen³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…Use of FTA cartridges for HPV testing has been attempted before and shown the solid carrier as a promising device for collection of cervical specimens for screening, however, a highly sensitive method for HPV detection which are amplification based was suggested (Gustavsson et al, 2009;de Bie et al, 2011). This cartridge has been shown previously to be useful in tropical condition (Phongsavan et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Use of Fast Transfer Analysis Cartridges for Cervical Sampling and Real Time PCR Based High Risk HPV Testing in Cervical Cancer Prevention - a Feasibility Study from South India

Ramshankar

Thangaraj²,

Malliga³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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Use of FTA card for dry collection, transportation and storage of cervical cell specimen to detect high-risk HPV

Cited by 74 publications

References 23 publications

Evaluation of the FTA Carrier Device for Human Papillomavirus Testing in Developing Countries

Evaluation of the FTA Carrier Device for Human Papillomavirus Testing in Developing Countries

Clinical Evaluation of Human Papillomavirus Detection by careHPV^TMTest on Physician-Samples and Self-Samples using The Indicating FTA Elute® Card

Use of Fast Transfer Analysis Cartridges for Cervical Sampling and Real Time PCR Based High Risk HPV Testing in Cervical Cancer Prevention - a Feasibility Study from South India

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Use of FTA card for dry collection, transportation and storage of cervical cell specimen to detect high-risk HPV

Cited by 74 publications

References 23 publications

Evaluation of the FTA Carrier Device for Human Papillomavirus Testing in Developing Countries

Evaluation of the FTA Carrier Device for Human Papillomavirus Testing in Developing Countries

Clinical Evaluation of Human Papillomavirus Detection by careHPVTMTest on Physician-Samples and Self-Samples using The Indicating FTA Elute® Card

Use of Fast Transfer Analysis Cartridges for Cervical Sampling and Real Time PCR Based High Risk HPV Testing in Cervical Cancer Prevention - a Feasibility Study from South India

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Product

Resources

About

Clinical Evaluation of Human Papillomavirus Detection by careHPV^TMTest on Physician-Samples and Self-Samples using The Indicating FTA Elute® Card