Use of Fourier-Transform Infrared Spectroscopy for the Diagnosis of Failure of Transfer of Passive Immunity and Measurement of Immunoglobulin Concentrations in Horses

Riley, Christopher B.; McClure, J. Trenton; Low-Ying, Sarah; Shaw, R. Anthony

doi:10.1892/0891-6640(2007)21[828:uofisf]2.0.co;2

Cited by 14 publications

(47 citation statements)

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“…2). In comparison, these values are higher than those reported previously for counterpart TIR-based assays for equid IgG 21,32 and camelid IgG levels, 3 and for the ATR-IR method described for canine IgG concentrations. 40 Furthermore, the correlation coefficients between IgG concentrations predicted by both IR spectroscopic methods and the reference RID IgG assay were higher than those reported for refractometry, 9,28 similar to those of near-IR spectroscopy, 52 but lower than the value reported for automated turbidimetric immunoassay.…”

Section: Discussioncontrasting

confidence: 68%

“…15,24 The positive and negative predictive values (PPV and NPV, respectively) vary with both test performance and the population FTPI prevalence. 32 In North America, the reported prevalence of FTPI in dairy calves ranges from 19% to 40%. 29,49 When the proportion of calves with FTPI increases, NPV decreases.…”

Section: Discussionmentioning

confidence: 99%

“…After dilution, 6 replicates were made for each serum sample by evenly spreading 10-μL aliquots of diluted sample onto 5 mm in diameter wells within an adhesive-masked, 96-well silicon microplate. 32 An empty well served as the background reference for each microplate. The loaded microplates were allowed to dry at room temperature (20-24°C) for 2 hr, resulting in thin dried films.…”

Section: Transmission Infrared Spectroscopymentioning

confidence: 99%

“…Thus, RID is not practical for routine, on-farm monitoring of the adequacy of transfer of passive immunity. 2,32 Infrared (IR) spectroscopy in combination with multivariate data analysis has emerged as an alternative technique for assessing FTPI in several species and has the following advantages: small sample volume requirement, rapid turnaround time, minimal disposable materials, low cost, and robust predictions. [41][42][43] Methods employing IR spectroscopy have been successfully used to determine nutrient contents of milk, 36,37 colostrum IgG concentration, 33 feed composition, and the kinetics of nutrient degradation in the rumen.…”

Section: Introductionmentioning

confidence: 99%

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Quantification of bovine immunoglobulin G using transmission and attenuated total reflectance infrared spectroscopy

et al. 2015

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Abstract. In this study, we evaluated and compared the performance of transmission and attenuated total reflectance (ATR) infrared (IR) spectroscopic methods (in combination with quantification algorithms previously developed using partial least squares regression) for the rapid measurement of bovine serum immunoglobulin G (IgG) concentration, and detection of failure of transfer of passive immunity (FTPI) in dairy calves. Serum samples (n = 200) were collected from Holstein calves 1-11 days of age. Serum IgG concentrations were measured by the reference method of radial immunodiffusion (RID) assay, transmission IR (TIR) and ATR-IR spectroscopy-based assays. The mean IgG concentration measured by RID was 17.22 g/L (SD ±9.60). The mean IgG concentrations predicted by TIR and ATR-IR spectroscopy methods were 15.60 g/L (SD ±8.15) and 15.94 g/L (SD ±8.66), respectively. RID IgG concentrations were positively correlated with IgG levels predicted by TIR (r = 0.94) and ATR-IR (r = 0.92). The correlation between 2 IR spectroscopic methods was 0.94. Using an IgG concentration <10 g/L as the cut-point for FTPI cases, the overall agreement between TIR and ATR-IR methods was 94%, with a corresponding kappa value of 0.84. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy for identifying FTPI by TIR were 0.87, 0.97, 0.91, 0.95, and 0.94, respectively. Corresponding values for ATR-IR were 0.87, 0.95, 0.86, 0.95, and 0.93, respectively. Both TIR and ATR-IR spectroscopic approaches can be used for rapid quantification of IgG level in neonatal bovine serum and for diagnosis of FTPI in dairy calves.

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Section: Discussioncontrasting

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Section: Transmission Infrared Spectroscopymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Quantification of bovine immunoglobulin G using transmission and attenuated total reflectance infrared spectroscopy

et al. 2015

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“…Since they are nondestructive tests, there is no need for a sample preparation and, therefore, they can be automated. 14 Riley et al 15 reported the use of the Fourier transform IR spectroscopy technique in conjunction to a partial least-squares ͑PLS͒ regression model to diagnose the failure of transfer of passive immunity in horses. They found sensitivity, specificity, accuracy, and predictive values for diagnosis as good as those obtained by using the radial immunodiffusion assay ͑RID͒.…”

Section: Introductionmentioning

confidence: 99%

Near-infrared Raman spectroscopy to detect anti-Toxoplasma gondii antibody in blood sera of domestic cats: quantitative analysis based on partial least-squares multivariate statistics

et al. 2010

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Abstract. Toxoplasmosis is an important zoonosis in public health because domestic cats are the main agents responsible for the transmission of this disease in Brazil. We investigate a method for diagnosing toxoplasmosis based on Raman spectroscopy. Dispersive nearinfrared Raman spectra are used to quantify anti-Toxoplasma gondii ͑IgG͒ antibodies in blood sera from domestic cats. An 830-nm laser is used for sample excitation, and a dispersive spectrometer is used to detect the Raman scattering. A serological test is performed in all serum samples by the enzyme-linked immunosorbent assay ͑ELISA͒ for validation. Raman spectra are taken from 59 blood serum samples and a quantification model is implemented based on partial least squares ͑PLS͒ to quantify the sample's serology by Raman spectra compared to the results provided by the ELISA test. Based on the serological values provided by the Raman/PLS model, diagnostic parameters such as sensitivity, specificity, accuracy, positive prediction values, and negative prediction values are calculated to discriminate negative from positive samples, obtaining 100, 80, 90, 83.3, and 100%, respectively. Raman spectroscopy, associated with the PLS, is promising as a serological assay for toxoplasmosis, enabling fast and sensitive diagnosis.

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Infrared Spectroscopy of Biofluids in Clinical Chemistry and Medical Diagnostics

Shaw

Low-Ying

Man

et al. 2007

Biomedical Vibrational Spectroscopy

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Use of Fourier-Transform Infrared Spectroscopy for the Diagnosis of Failure of Transfer of Passive Immunity and Measurement of Immunoglobulin Concentrations in Horses

Abstract: The overall performance of the IR-based test was similar to that of the colorimetric assay and was superior and more economic than other available tests.

Cited by 14 publications

References 0 publications

Quantification of bovine immunoglobulin G using transmission and attenuated total reflectance infrared spectroscopy

Quantification of bovine immunoglobulin G using transmission and attenuated total reflectance infrared spectroscopy

Near-infrared Raman spectroscopy to detect anti-Toxoplasma gondii antibody in blood sera of domestic cats: quantitative analysis based on partial least-squares multivariate statistics

Infrared Spectroscopy of Biofluids in Clinical Chemistry and Medical Diagnostics

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