Use of Egg Yolk in Serological Tests (ELISA and HI) to Detect Antibody to Newcastle Disease, Infectious Bronchitis, and Mycoplasma gallisepticum

Abstract

Serum and yolks from commercial flocks and from hens exposed to Newcastle disease virus (NDV), infectious bronchitis virus (IBV), and Mycoplasma gallisepticum (MG) were tested for immunoglobulin G antibody by the enzyme-linked immunosorbent assay (ELISA) and the hemagglutination-inhibition (HI) test. Yolks prepared by chloroform extraction and low-speed centrifugation performed well in the serological tests used and were a suitable alternative to serum for antibody determination by the ELISA for NDV, IBV, and MG and by HI test for NDV.

Persistence and vertical transmission of chicken anaemia agent in experimentally infected laying hens

“…Yolk antibody was prepared according to the method of Piela et al (1984). Briefly, 0.5 ml yolk was mixed with 0.5 ml PBS and 2 ml chloroform was added.…”

ELISAs and the serological analysis of salmonella infections in poultry: a review

“…Because of its high IgG concentrations egg-yolk [29,30] rather than serum has already been applied to other avian pathogens such as Newcastle disease virus, infectious bronchitis virus [31] and mycoplasma [31,32]. It has been applied to S. enteritidis, typhimurium and gallinarum [24,27,28,33,34].…”

Detection of antibody to avian viruses in human populations

“…Viruses ELISA procedure The indirect sandwich ELISA method for assay of antibody was used as has been described elsewhere (1,2,9). The ELISA assay was performed in polystyrene Micro-ELISA plates with 96 flat-bottomed wells (Dynatech Laboratories, South Windham, ME, USA).…”

“…The sera were collected between 1983 and 1985 and tested using ELISA testing. which offers a more sensitive method of antibody detection than any of the previously use(d serological tests (1,2).…”