Persistence and vertical transmission of chicken anaemia agent in experimentally infected laying hens

Hoop, Richard

doi:10.1080/03079459208418867

Cited by 59 publications

(39 citation statements)

References 29 publications

(27 reference statements)

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“…Infection of adult chicks with CIAV generally does not cause clinical disease albeit subclinical infections frequently occur which have been found to be associated with significant economic losses Hoop 1992;Hagood et al 2000;Dhama et al 2008). The transmission and spread of CIAV infections in adult birds occur through horizontal spread, which does not induce clinical disease (chicken infection anaemia).…”

Section: Discussionmentioning

confidence: 99%

Correlation between cytokine profile,antibody titre and viral load during sub-clinical chicken anaemia virus infection

Wani¹,

Dhama²,

Latheef³

et al. 2014

Vet. Med. - Czech

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Th e present work was carried out to investigate the correlation between viral load and the cytokine profi le and virus specifi c antibody levels at fi ve day intervals up to 25 days post infection (dpi) in various tissues of chicks infected with chicken infectious anaemia virus (CIAV) at six weeks of age (sub-clinical infection). For determining the viral load in the various tissues, recombinant plasmid VP2-pET32b was prepared and serially diluted tenfold for the generation of a standard curve using real time polymerase chain reaction (PCR). Cytokine fold changes in mRNA levels of IFN-γ, IL-1, IL-2, IL-12 in the spleen and IFN-γ, IL-12 in freshly collected whole blood were determined. Th e results showed that peak viral load occurs between 10-20 days post infection in the lymphoid tissues viz., thymus, spleen, liver, bone marrow, bursa and also blood, being highest in blood followed by thymus. A varied response in the expression levels of individual cytokines was observed during all the intervals post infection. Th e IFN-γ increased two to fi ve fold in blood and spleen while IL-2 decreased in the splenic tissue during the same period. Peak IFN-γ coincided with peak viral load in the spleen at 10 dpi while in blood it peaked earlier at fi ve days post infection and remained high during the peak viral load at 10-20 dpi. Virus specifi c antibodies were signifi cantly higher at 15 dpi and were thereafter found to be strongly associated with viral load regression although higher concentrations of virus remained in the blood and thymic tissues until 25 dpi, indicating a role of other immune components in virus clearance. In conclusion, this study reports a negative correlation of viral load and the progression of the antibody response and IFN-γ cytokine expression in chicks infected with CIAV during their sub-clinical infectious stage. However, cytokine expression from the various individual immune cells like monocytes, heterophils, CD4 + T, CD8 + T cells and others during the pathogenesis of this immunosuppressive poultry pathogen remains to be elucidated.

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Section: Discussionmentioning

confidence: 99%

Correlation between cytokine profile,antibody titre and viral load during sub-clinical chicken anaemia virus infection

Wani¹,

Dhama²,

Latheef³

et al. 2014

Vet. Med. - Czech

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“…Therefore, the disease associated with CAV infection has been prevented by immunization of breeding flocks with live virus vaccines. As vertical transmission through the hatching egg is suspected to be the most important means of transmission of CAV infection (Chettle et al, 1989 ;Hoop, 1992), vaccine strains should have low pathogenicity even for young chickens. This paper describes the generation of a low-pathogenic cloned virus by molecular cloning of CAV DNA from a lowpassage CAV in cell culture and identification of the pathogenicity determinant in the genome of CAV.…”

Section: Introductionmentioning

confidence: 99%

Identification of a genetic determinant of pathogenicity in chicken anaemia virus

Yamaguchi

Imada

Kaji

et al. 2001

Journal of General Virology

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The molecular basis of pathogenicity of the chicken anaemia virus (CAV) needs to be clarified in order to develop a safe, live virus vaccine. In this study, several high-and low-pathogenic infectious DNA clones were obtained from field virus samples after 12 or 38 passages in MDCC-MSB1 cells. The high-pathogenic clones induced a low haematocrit, low weight gain and high mortality. Nucleotide sequence analyses identified one amino acid, at residue 394 of the VP1 capsid protein, as a major determinant of pathogenicity. To determine the role of this amino acid in pathogenicity, chimeric infectious DNA clones and point-mutated clones were used for chicken pathogenicity tests. These analyses clearly demonstrated that residue 394 of VP1 was crucial for the pathogenicity of CAV ; all of the cloned viruses with glutamine at this position were highly pathogenic, whereas those with histidine had low pathogenicity. Low-pathogenic CAV, based on an infectious DNA clone, is a candidate for a genetically homogeneous and stable CAV live vaccine.

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“…Este foi o primeiro trabalho que descreveu a detecção do DNA do CAV a partir de cama aviária. O CAV é resistente no meio ambiente (Schat 2003), além de ser excretado nas fezes em grandes quantidades (Hoop 1992). Desta maneira, a utilização de cama aviária para a detecção do vírus possui algumas vantagens, já que não requer o sacrifício e nem necropsias de animais, além de permitir a amostragem de vários animais de um mesmo lote.…”

Section: Discussão E Conclusões Discussão E Conclusões Discussão E Counclassified

Um protocolo de "nested-PCR" para detecção do vírus da anemia das galinhas

et al. 2005

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Persistence and vertical transmission of chicken anaemia agent in experimentally infected laying hens

Cited by 59 publications

References 29 publications

Correlation between cytokine profile,antibody titre and viral load during sub-clinical chicken anaemia virus infection

Correlation between cytokine profile,antibody titre and viral load during sub-clinical chicken anaemia virus infection

Identification of a genetic determinant of pathogenicity in chicken anaemia virus

Um protocolo de "nested-PCR" para detecção do vírus da anemia das galinhas

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