Use of Cystatin C Determination in Clinical Diagnostics

Mareś, Jan; Stejskal, David; Vavrouskova, J.; Urbánek, Karel; Herzig, Roman; Hluštı́k, Petr

doi:10.5507/bp.2003.025

Cited by 28 publications

(35 citation statements)

References 32 publications

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“…There are some the extra-renal factors such as melanoma, colon cancer and HIV infections increasing sCys-C levels in human (Randers and Erlandsen 1999;Mares et al 2003). In veterinary medicine, there are limited studies on dogs with renal diseases indicating the importance of sCys-C with serum creatinine and urea nitrogen comparison.…”

Section: Discussionmentioning

confidence: 97%

Evaluation of serum cystatin-C in dogs with visceral leishmaniasis

et al. 2009

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Serum Cystatin C (sCys-C) is one of the most important serum markers of renal function assessment in dogs. The purpose of this study was to determine the sCys-C concentration in dogs with visceral leishmaniasis (VL). In the study, 16 dogs with VL and 10 clinical healty dogs (control) were used. Mean sCys-C concentration of the infected dogs was significantly higher than that of the control group (p < 0.05). Mean serum creatinine concentration was lower and mean blood urea nitrogen, albumin and globulin concentrations were higher in dogs with VL; however, these changes were not statistically significant. Mean total protein and phosphorus concentrations were found to be higher in dogs with VL than healthy dogs (p < 0.05). No significant correlation had been determined between sCys-C and other variables. Visceral leishmaniasis in dogs has increased sCys-C concentration indicating a possible renal impairment; however, further studies are needed to be performed together with renal biopsies in the investigation sCys-C in dogs with VL.

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Section: Discussionmentioning

confidence: 97%

Evaluation of serum cystatin-C in dogs with visceral leishmaniasis

et al. 2009

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“…It predicts secondary cardiac events other than heart failure in adults with known coronary heart disease independently of serum creatinine levels or creatinine clearance levels [36]. Elevated cystatin C is also associated with hyper-homocysteinemia [37], a well-known cardiac risk factor. On the other hand, adult population-based studies demonstrated that age, male gender, weight, height, cigarette smoking, higher serum Creactive protein and rheumatoid arthritis were independently associated with elevated cystatin C levels after adjustment for kidney function [38,39].…”

Section: Discussionmentioning

confidence: 99%

Serum cystatin C and left ventricular diastolic dysfunction in children with chronic kidney disease

et al. 2006

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Previous studies indicate that serum cystatin C predicts incident heart failure in older adults. Children with chronic kidney disease (CKD) develop left ventricular (LV) diastolic dysfunction, often the initial abnormality of cardiac function. We hypothesized that cystatin C might predict LV diastolic dysfunction in children with CKD. Fifty-seven subjects, aged 6-21 years, with stage 2-4 CKD underwent echocardiography. Diastole was assessed from transmitral Doppler [maximum early (E wave) and late (A wave) diastolic flow velocities (E/A ratio)] and from tissue Doppler [septal mitral annular peak velocities (E')]. LV filling pressures were determined, using a ratio of E/E'. Fourteen (25%) patients had low E' and 15 (26%) had high E/E'. Children with abnormal E' or E/E' had significantly higher cystatin C levels than children with normal indices (P<0.05). Neither serum creatinine nor measured glomerular filtration rate (GFR) significantly correlated with E' or E/E'. Stepwise multiple regression analysis showed that cystatin C (beta=-0.825, P=0.023) and left ventricular mass (LVM) index (beta=0.099, P=0.006) independently predicted E'; LVM index independently predicted E/E' (beta=0.0173, P=0.01). We conclude that, in contrast to measured GFR or serum creatinine level, elevated serum cystatin C might be associated with diastolic dysfunction in children with CKD.

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“…1). The increasing interest of cystatin C as an early and new marker of kidney dysfunction, superior to plasma creatinine [9][10][11][12], moreover makes it an interesting choice from a clinical point of view. Until now, plasma creatinine is mostly used to estimate the glomererular filtration rate (GFR).…”

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confidence: 99%

The use of tryptic marker‐peptides for the quantitative analysis of Cystatin C

Storme

Sinnaeve

Bocxlaer

2005

J of Separation Science

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The use of marker-peptides, measured by LC-MS/MS, is investigated for the quantitative analysis of proteins. To that end, cystatin C is chosen as a model protein. It not only functions as a proof of concept protein but the growing interest in cystatin C as a new marker of kidney failure provides a practical application at the same time. The use of trypsin-based proteolysis, to obtain so-called marker-peptides, simplifies the quantification of a protein to the quantification of a single or a number of peptides. Reproducibility of the trypsin proteolysis procedure is vital and has been optimised. A number of the marker-peptides obtained are selected for LC-MS(/MS) analysis. They are completely separated by high-pressure LC allowing maximum selectivity and mass spectrometric multiple reaction monitoring sensitivity. By doing so, linear calibration curves can be obtained for cystatin C over two orders of magnitude. Experiments have been performed on a triple quadrupole mass spectrometer by single ion monitoring (maximum sensitivity) as well as by multiple reaction monitoring (maximum specificity).

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Use of Cystatin C Determination in Clinical Diagnostics

Cited by 28 publications

References 32 publications

Evaluation of serum cystatin-C in dogs with visceral leishmaniasis

Evaluation of serum cystatin-C in dogs with visceral leishmaniasis

Serum cystatin C and left ventricular diastolic dysfunction in children with chronic kidney disease

The use of tryptic marker‐peptides for the quantitative analysis of Cystatin C

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