Use of Combusted Natural Gas to Cultivate the Anaerobic Bacterial Flora from the Cecum Contents of Mice

Koopman, J. P.; Oeveren, J. P. Van; Janssen, F. G. J.

doi:10.1128/aem.26.4.584-588.1973

Cited by 14 publications

(4 citation statements)

References 5 publications

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“…The Bifidobacteria were identified on the basis of their pigment‐forming characteristics and had been verified earlier by the detection of fructose‐6‐phosphate phosphoketolase. The Columbia Agar Base, Mannitol Salt agar, Reinforced Clostridial Agar, Bacteroides Bile Esculine and Brucella Blood Agar were incubated during 48 h at 37 ∘ C in an anaerobic glove box as described earlier ( Koopman et al, 1973). The other media were incubated during 24 h under aerobic circumstances at 37 ∘ C. The total bacterial counts were performed in Gram‐stained slides as described by Holdeman and Moore (1972).…”

Section: Microbial Culture Of Faecesmentioning

confidence: 99%

Faecal bacterial profile, nitrogen excretion and mineral absorption in healthy dogs fed supplemental oligofructose

Beynen¹,

Baas²,

Hoekemeijer³

et al. 2002

Animal Physiology Nutrition

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In a cross-over trial, five healthy dogs were fed a dry food without or with 1% (w/w) oligofructose to assess any oligofructose-induced effects on the faecal bacterial profile, nitrogen excretion and mineral absorption. The diets were given for a period of 3 weeks. Oligofructose feeding significantly raised the number of Bifidobacteria, Streptococci and Clostridia in faeces. The numbers of faecal anaerobic and aerobic bacteria were raised after ingestion of oligofructose. The faecal pH was unchanged. There was no effect of oligofructose feeding on the route of nitrogen excretion which was associated with a lack of effect on faecal ammonium and urinary urea excretion. It is suggested that the absence or presence of an effect of oligofructose on urinary and faecal nitrogen excretion depends on the background composition of the diet, in particular the content of non-digestible, fermentable carbohydrates. In the diets used, the content of non-digestible, fermentable carbohydrates was not measured. Both apparent magnesium and calcium absorption were significantly raised by oligofructose feeding, but phosphorus absorption was unaffected. The data presented may contribute to the qualification of the use of oligofructose in dog foods.

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Section: Microbial Culture Of Faecesmentioning

confidence: 99%

Faecal bacterial profile, nitrogen excretion and mineral absorption in healthy dogs fed supplemental oligofructose

Beynen¹,

Baas²,

Hoekemeijer³

et al. 2002

Animal Physiology Nutrition

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“…The procedure was continued under anaerobic conditions with an oxygen concentration of less than 5 p.p.m. [19]. After homogenization, different concentrations of the homogenate were spread on a non-selective medium containing either 22.5 g agar or a 7% concentration of blood obtained from sheep.…”

Section: Bacterial Examination Of Jejunal Fluidmentioning

confidence: 99%

Unconjugated serum bile acids as a marker of small intestinal bacterial overgrowth

Masclee¹,

Tangerman²,

Schaik³

et al. 1989

Eur J Clin Investigation

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Non-invasive methods to detect small intestinal bacterial overgrowth often lack specificity in patients who have undergone an ileal resection or have an accelerated intestinal transit. Since elevated serum unconjugated bile acid levels have been found in patients with clinical signs of bacterial overgrowth, we studied the clinical value of unconjugated serum bile acids as a marker of small intestinal bacterial overgrowth. Patients with culture-proven bacterial overgrowth had significantly elevated fasting unconjugated serum bile acid levels (median and range: 4.5; 1.4-21.5 mumol l-1) as compared to healthy subjects (0.9; 0.3-1.7 mumol l-1, P less than 0.005), to persons with an accelerated intestinal transit (1.0; 0.3-1.9 mumol l-1, P less than 0.005) and to persons who have undergone an ileal resection (2.1; 0.7-3.6 mumol l-1, P less than 0.005). The same was true 30 and 60 min after ingestion of a Lundh meal. Serum unconjugated bile acid levels above 4 mumol l-1 were found in eight of 10 patients with culture-proven small intestinal bacterial overgrowth whereas serum levels above 4 mumol l-1 were found in none of the patients from the three control groups. These results suggest that determination of unconjugated serum bile acids is of clinical value in the evaluation of patients suspected of small intestine bacterial overgrowth.

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“…The donor chickens were killed by cervical dislocation. Immediately hereafter parts of crop, ileum, caecum, and colon were ligated, removed, weighed, and introduced into an anaerobic glove-box (6). The samples were homogenized in 4.5 ml broth (8) and tenfold dilutions were made.…”

Section: Microbiological Techniquesmentioning

confidence: 99%

Protection of chicks againstSalmonella infantisinfection induced by strict anaerobically cultured intestinal microflora

Goren¹,

Jong²,

Doornenbal³

et al. 1984

Veterinary Quarterly

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In two experiments newly hatched broilers were orally inoculated either with intestinal microfloras cultured under different conditions or with a suspension of intestinal homogenate and challenged with high doses (3 X 10(5) c.f.u. per chick) of Salmonella infantis organisms. Inocula were prepared from intestinal material of mature SPF WL hens under aerobic or strictly anaerobic conditions (less than 5 ppm oxygen), and protected against atmospheric oxygen during storage. A very significant reduction of the number of Salmonella infantis bacteria in caecal content was achieved one and two weeks after challenge in those groups of chicks which were inoculated with anaerobically cultured flora or with intestinal homogenate. In order to induce protection, obligate anaerobes had to be accompanied by facultative anaerobes. Amprolium or Monensin dit not interfere with protection. Protection induced by intestinal microflora depends on anaerobiosis during preparation, culturing, storage, and application of the protective bacteria. Starvation on the first day of life appeared to have a negative effect on protection.

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Use of Combusted Natural Gas to Cultivate the Anaerobic Bacterial Flora from the Cecum Contents of Mice

Cited by 14 publications

References 5 publications

Faecal bacterial profile, nitrogen excretion and mineral absorption in healthy dogs fed supplemental oligofructose

Faecal bacterial profile, nitrogen excretion and mineral absorption in healthy dogs fed supplemental oligofructose

Unconjugated serum bile acids as a marker of small intestinal bacterial overgrowth

Protection of chicks againstSalmonella infantisinfection induced by strict anaerobically cultured intestinal microflora

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