Use of collagen/chitosan sponges mineralized with hydroxyapatite for the repair of cranial defects in rats

Munhoz, M.A.S.; Hirata, Helton Hiroshi; Plepis, Ana Maria de Guzzi; Martins, Virgínia da Conceição Amaro; Cunha, Marcelo Rodrigues da

doi:10.1016/j.injury.2018.09.018

Cited by 28 publications

(26 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Synthetic hydroxyapatite. The hydroxyapatite evaluated in the present study is a microgranular ceramic, synthesized by the São Carlos Institute of Chemistry-USP, with particle size < 0.149 mm., Ca/P ratio obtained by EDX was 1.89 ± 0.04 and the major elements found were carbon (0.2 keV), oxygen (0.5 keV), calcium (3.7 and 4.0 keV), and phosphorus (2.0 keV) [13]. The sterilization of the biomaterial was by ethylene oxide gas.…”

Section: Methodsmentioning

confidence: 95%

Elastin-derived scaffolding associated or not with bone morphogenetic protein (BMP) or hydroxyapatite (HA) in the repair process of metaphyseal bone defects

et al. 2020

View full text Add to dashboard Cite

Tissue engineering represents a promising alternative for reconstructive surgical procedures especially for the repair of bone defects that do not regenerate spontaneously. The present study aimed to evaluate the effects of the elastin matrix (E24/50 and E96/37) incorporated with hydroxyapatite (HA) or morphogenetic protein (BMP) on the bone repair process in the distal metaphysis of rat femur. The groups were: control group (CG), hydrolyzed elastin matrix at 50˚C/24h (E24/50), E24/50 + HA (E24/50/HA), E24/50 + BMP (E24/50/ BMP), hydrolyzed elastin matrix at 37˚C/96h (E96/37), E96/37 + HA (E96/37/HA), E96/37 + BMP (E96/37/BMP). Macroscopic and radiographic analyses showed longitudinal integrity of the femur in all groups without fractures or bone deformities. Microtomographically, all groups demonstrated partial closure by mineralized tissue except for the E96/37/HA group with hyperdense thin bridge formation interconnecting the edges of the ruptured cortical. Histologically, there was no complete cortical recovery in any group, but partial closure with trabecular bone. In defects filled with biomaterials, no chronic inflammatory response or foreign body type was observed. The mean volume of new bone formed was statistically significant higher in the E96/37/HA and E24/50 groups (71.28 ± 4.26 and 66.40 ± 3.69, respectively) than all the others. In the confocal analysis, it was observed that all groups

show abstract

Section: Methodsmentioning

confidence: 95%

Elastin-derived scaffolding associated or not with bone morphogenetic protein (BMP) or hydroxyapatite (HA) in the repair process of metaphyseal bone defects

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Stoichiometric hydroxylapatite (Ca 10 (PO 4 ) 6 (OH) 2 ) is the most similar material to the mineral component of human hard tissues and therefore it is considered the ideal substance for bone defects restorations [25,26,27]. Keeping the same geometry while accepting a big variety of anions and cations is one of the most important structural characteristics of hydroxylapatite [28,29].…”

Section: Introductionmentioning

confidence: 99%

Photoluminescent Hydroxylapatite: Eu3+ Doping Effect on Biological Behaviour

et al. 2019

View full text Add to dashboard Cite

Luminescent europium-doped hydroxylapatite (EuXHAp) nanomaterials were successfully obtained by co-precipitation method at low temperature. The morphological, structural and optical properties were investigated by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier Transform Infrared (FT-IR), UV-Vis and photoluminescence (PL) spectroscopy. The cytotoxicity and biocompatibility of EuXHAp were also evaluated using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)) assay, oxidative stress assessment and fluorescent microscopy. The results reveal that the Eu3+ has successfully doped the hexagonal lattice of hydroxylapatite. By enhancing the optical features, these EuXHAp materials demonstrated superior efficiency to become fluorescent labelling materials for bioimaging applications.

show abstract

“…The manufacturing process of elastin and collagen matrices and their characterizations have been described in greater detail in previously published studies [7,19,27,[40][41][42]. The elastin matrices used were prepared by the Biochemistry and Biomaterials Group of the São Carlos Institute of Chemistry (University of São Paulo-USP, Brazil) by adapting the methodology used to obtain collagen [7,8,14]. In summary, the cartilages were treated with an alkaline solution, a mechanism known as hydrolysis, with salts sulfates and chlorides and alkaline earth alkali metal hydroxides at 37 • C for 24 h (E24/37).…”

Section: Elastin Matrix Derived From Bovine Auricular Cartilagementioning

confidence: 99%

“…Natural polymers, derived from collagen and elastin, have different shapes and densities, which simulate the native interstitial space, helping cell adhesion and supporting the proliferation and differentiation of new cell types [7,8]. Collagen is an insoluble fibrous protein considered as the main structural protein in most hard and soft tissues in animals.…”

Section: Introductionmentioning

confidence: 99%

In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair Process

et al. 2021

View full text Add to dashboard Cite

Autologous bone grafts, used mainly in extensive bone loss, are considered the gold standard treatment in regenerative medicine, but still have limitations mainly in relation to the amount of bone available, donor area, morbidity and creation of additional surgical area. This fact encourages tissue engineering in relation to the need to develop new biomaterials, from sources other than the individual himself. Therefore, the present study aimed to investigate the effects of an elastin and collagen matrix on the bone repair process in critical size defects in rat calvaria. The animals (Wistar rats, n = 30) were submitted to a surgical procedure to create the bone defect and were divided into three groups: Control Group (CG, n = 10), defects filled with blood clot; E24/37 Group (E24/37, n = 10), defects filled with bovine elastin matrix hydrolyzed for 24 h at 37 °C and C24/25 Group (C24/25, n = 10), defects filled with porcine collagen matrix hydrolyzed for 24 h at 25 °C. Macroscopic and radiographic analyses demonstrated the absence of inflammatory signs and infection. Microtomographical 2D and 3D images showed centripetal bone growth and restricted margins of the bone defect. Histologically, the images confirmed the pattern of bone deposition at the margins of the remaining bone and without complete closure by bone tissue. In the morphometric analysis, the groups E24/37 and C24/25 (13.68 ± 1.44; 53.20 ± 4.47, respectively) showed statistically significant differences in relation to the CG (5.86 ± 2.87). It was concluded that the matrices used as scaffolds are biocompatible and increase the formation of new bone in a critical size defect, with greater formation in the polymer derived from the intestinal serous layer of porcine origin (C24/25).

show abstract

Use of collagen/chitosan sponges mineralized with hydroxyapatite for the repair of cranial defects in rats

Cited by 28 publications

References 29 publications

Elastin-derived scaffolding associated or not with bone morphogenetic protein (BMP) or hydroxyapatite (HA) in the repair process of metaphyseal bone defects

Elastin-derived scaffolding associated or not with bone morphogenetic protein (BMP) or hydroxyapatite (HA) in the repair process of metaphyseal bone defects

Photoluminescent Hydroxylapatite: Eu3+ Doping Effect on Biological Behaviour

In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair Process

Contact Info

Product

Resources

About