Use of COI, CytB and ND5 genes for intra- and inter-specific differentiation of Haematobia irritans and Haematobia exigua

Low, Van Lun; Tan, Tiong Kai; Lim, Phaik Eem; Domingues, Luísa N.; Tay, Sun Tee; Lim, Yvonne Ai Lian; Goh, Thary Gazi; Panchadcharam, Chandrawathani; Bathmanaban, Premaalatha; Sofian‐Azirun, Mohd

doi:10.1016/j.vetpar.2014.05.036

Cited by 14 publications

(12 citation statements)

References 15 publications

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“…Of several mitochondrial and nuclear genes tested in our previous study 6 , the mitochondrial cytochrome c oxidase subunit I (COI), cytochrome b (Cytb) and NADH dehydrogenase subunit 5 (ND5) genes were found to be more variable and informative in resolving the intra- and inter-specific relationships between H. exigua and H. irritans . These three genes were therefore adopted in the present study for a preliminary assessment of the genetic divergence of H. exigua collected from various geographic regions.…”

Section: Resultsmentioning

confidence: 90%

“…The taxonomic status of Haematobia taxa has long been questioned because of their similar morphological characteristics that cannot easily be distinguished 6, 13 . Although the morphological differences between both taxa are minor, H. exigua differs from H. irritans by the presence of 4 to 6 distinctive long hairs with curled tips on the segments of the male’s hind tarsus 13 .…”

Section: Discussionmentioning

confidence: 99%

“…The congener H. irritans analyzed from our previous study 6 was also included for phylogenetic inference. Species identification was performed using morphological keys 13 .…”

Section: Methodsmentioning

confidence: 99%

“…6 . The PCR products were then sequenced in forward and reverse directions using BIG DYE Terminator v3.1 by an ABI 3730XL Genetic Analyzer (Applied Biosystems Inc., Foster City, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

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Contrasting evolutionary patterns between two haplogroups of Haematobia exigua (Diptera: Muscidae) from the mainland and islands of Southeast Asia

Low

Tan

Prakash

et al. 2017

Sci Rep

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Uncovering the hidden diversity and evolutionary history of arthropods of medico-veterinary importance could have significant implications for vector-borne disease control and epidemiological intervention. The buffalo fly Haematobia exigua is an obligate bloodsucking ectoparasite of livestock. As an initial step towards understanding its population structures and biogeographic patterns, we characterized partial cytochrome c oxidase subunit I (COI) and cytochrome b (Cytb) sequences of H. exigua from three distinct geographic regions in Southeast Asia. We detected two distinct mitochondrial haplogroups of H. exigua in our surveyed geographic regions. Haplogroup I is widespread in the Southeast Asian mainland whereas haplogroup II is generally restricted to the type population Java Island. Both haplogroups were detected co-occurring on Borneo Island. Additionally, both haplogroups have undergone contrasting evolutionary histories, with haplogroup I exhibited a high level of mitochondrial diversity indicating a population expansion during the Pleistocene era dating back to 98,000 years ago. However, haplogroup II presented a low level of mitochondrial diversity which argues against the hypothesis of recent demographic expansion.

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Section: Resultsmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

“…The congener H. irritans analyzed from our previous study 6 was also included for phylogenetic inference. Species identification was performed using morphological keys 13 .…”

Section: Methodsmentioning

confidence: 99%

“…6 . The PCR products were then sequenced in forward and reverse directions using BIG DYE Terminator v3.1 by an ABI 3730XL Genetic Analyzer (Applied Biosystems Inc., Foster City, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

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Contrasting evolutionary patterns between two haplogroups of Haematobia exigua (Diptera: Muscidae) from the mainland and islands of Southeast Asia

Low

Tan

Prakash

et al. 2017

Sci Rep

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“…The average haplotype diversity was 0.833 and nucleotide diversity was 0.025 in the studied species. Low et al . (2014) also calculated haplotype diversity between the range 0.733 to 0.961 and nucleotide diversity in range 0.0005 to 0.0014.…”

Section: Discussionmentioning

confidence: 99%

Molecular Identification of some Indian Muscid flies (Diptera: Muscidae) based on mitochondrial gene COII

Singh

Achint

2017

Preprint

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Taxonomic identification of flies having medical and veterinary importance is often complicated due to the lookalike morphological characters. Molecular identification of five Indian muscid flies (Musca domestica, Musca sorbens, Musca crassirostris, Stomoxys calcitransand Haematobia irritans) has been attempted on the basis of mitochondrial COII gene.Sequences of 500-520 bp were analysed and found to be A+T rich. Rate of transitions was higher than transversions. The average haplotype diversity was 0.833 and nucleotide diversity was 0.02547 within the different species, which were calculated with the DnaSP Version 5.0. The genetic distances calculated by K2P model, showed the interspecific distances range more than 8.2%, while the intraspecific distance range had not exceed 0.8%. The 1000 bootstrapped Neighbour-joining tree and Maximum likelihood tree were constructed to establish the phylogenetic relationship between the different muscid species. The results show the robustness . CC-BY-NC-ND 4.0 International license It is made available under a (which was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint . http://dx.doi.org/10.1101/208314 doi: bioRxiv preprint first posted online Oct. 24, 2017; of COII gene as a diagnostic marker. The data obtained from this study would be worthy for medical and veterinary entomologists for precise identification of imperative muscid species.

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Wolbachia successfully replicate in a newly established horn fly, Haematobia irritansirritans (L.) (Diptera: Muscidae) cell line

Madhav

Brown

Morgan

et al. 2020

Pest Management Science

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BACKGROUND: Haematobia spp., horn flies (HF) and buffalo flies (BF), are economically important ectoparasites of dairy and beef cattle. Control of these flies relies mainly on treating cattle with chemical insecticides. However, the development of resistance to commonly used compounds is compromising the effectiveness of these treatments and alternative methods of control are required. Wolbachia are maternally transmitted endosymbiotic bacteria of arthropods that cause various reproductive distortions and fitness effects, making them a potential candidate for use in the biological control of pests. The first step towards this is the establishment and adaptation of xenobiotic infections of Wolbachia in target host cell lines.RESULTS: Here, we report the successful establishment of a continuous HF cell line (HIE-18) from embryonic cells and its stable transinfection with Wolbachia strains wAlbB native to mosquitoes, and wMel and wMelPop native to Drosophila melanogaster. HIE-18 cells were typically round and diploid with ten chromosomes (2n = 10) or tetraploid with 20 chromosomes (4n = 20), with a doubling time of 67.2 h. Wolbachia density decreased significantly in HIE-18 cells in the first 48 h of infection, possibly due to overexpression of antimicrobial peptides through the Imd immune signalling pathway. However, density recovered after this time and HIE-18 cell lines stably infected with the three strains of Wolbachia have now each been subcultured more than 50 times as persistently infected lines.CONCLUSION: The amenability of HF cells to infection with different strains of Wolbachia and the establishment of stable sustaining infections suggest the potential for use of Wolbachia in novel approaches for the control of Haematobia spp. Further, the availability of the HIE-18 cell line will provide an important resource for the study of genetics, host-parasite interactions and chemical resistance in Haematobia populations.

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Use of COI, CytB and ND5 genes for intra- and inter-specific differentiation of Haematobia irritans and Haematobia exigua

Cited by 14 publications

References 15 publications

Contrasting evolutionary patterns between two haplogroups of Haematobia exigua (Diptera: Muscidae) from the mainland and islands of Southeast Asia

Contrasting evolutionary patterns between two haplogroups of Haematobia exigua (Diptera: Muscidae) from the mainland and islands of Southeast Asia

Molecular Identification of some Indian Muscid flies (Diptera: Muscidae) based on mitochondrial gene COII

Wolbachia successfully replicate in a newly established horn fly, Haematobia irritansirritans (L.) (Diptera: Muscidae) cell line

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