Molecular Identification of some Indian Muscid flies (Diptera: Muscidae) based on mitochondrial gene COII

Abstract

Taxonomic identification of flies having medical and veterinary importance is often complicated due to the lookalike morphological characters. Molecular identification of five Indian muscid flies (Musca domestica, Musca sorbens, Musca crassirostris, Stomoxys calcitransand Haematobia irritans) has been attempted on the basis of mitochondrial COII gene.Sequences of 500-520 bp were analysed and found to be A+T rich. Rate of transitions was higher than transversions. The average haplotype diversity was 0.833 and nucleotide diversity was 0.02547 within the different species, which were calculated with the DnaSP Version 5.0. The genetic distances calculated by K2P model, showed the interspecific distances range more than 8.2%, while the intraspecific distance range had not exceed 0.8%. The 1000 bootstrapped Neighbour-joining tree and Maximum likelihood tree were constructed to establish the phylogenetic relationship between the different muscid species. The results show the robustness . CC-BY-NC-ND 4.0 International license It is made available under a (which was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint . http://dx.doi.org/10.1101/208314 doi: bioRxiv preprint first posted online Oct. 24, 2017; of COII gene as a diagnostic marker. The data obtained from this study would be worthy for medical and veterinary entomologists for precise identification of imperative muscid species.

Molecular identification and characterization of medically and veterinary important flies of Bangladesh based on mitochondrial COI gene sequences

“…Phylogenetic analysis revealed that species belong to same family were in the same clade (Figure 4). Singh and Achint (2017) found that Musca species formed a sister clade with Haematobia irritans which supports our results. Iwasa and Ishiguro (2010) reported Haematobia and Musca as sister clade similar to our findings.…”

Muscidae (Diptera)- A Historical Perspective

“…A key to the subfamily, genus, and species is also provided by them. Molecular work on muscid flies was carried out by Malviya et al (2011Malviya et al ( , 2012Malviya et al ( , and 2015, and Singh and Achint (2017). Malviya et al (2012) conducted a study on genetic relatedness among different muscid fly species.…”

“…During their study, they used the RAPD-PCR technique to show the genetic relatedness among muscid fly species. Singh and Achint (2017) studied the molecular identification of muscid flies. On the basis of the mitochondrial COII gene, five flies of the family Muscidae were identified.…”