Use of ceriofast™ for monitoring the toxicity of industrial effluents: Comparison with the 48‐H acute <i>Ceriodaphnia</i> toxicity test and Microtox®

Jung, Keumhee; Bitton, Gabriel

doi:10.1002/etc.5620161110

Cited by 8 publications

References 9 publications

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Luminescent Bacterial Biosensors for the Rapid Detection of Toxicants

Davoren¹

2004

Water Encyclopedia

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There is an increasing demand for sensitive and user‐friendly biological assays for the rapid and cost effective assessment of environmental contaminants in soil, sediment, and water ecosystems. Luminescent bacterial biosensors have emerged as promising tools in environmental monitoring for the sensitive and specific quantification of both toxicity and bioavailability. The naturally luminescent marine bacterium Vibrio fischeri has been extensively employed to assess the toxicity of a wide range of pollutants. In addition, recent advances in reporter gene technology have led to the development of many luminescent‐marked bacterial biosensors. Some of the bacterial biosensors that have been constructed are used for general toxicity testing, as they constitutively express luminescence and respond to toxicants in a “lights off” manner. Another class of biosensors have been developed which react to toxicants in a “lights on” manner, where light production is induced by bioavailable concentrations of toxicants. These microbial biosensors offer a powerful new approach to environmental monitoring, providing an indication of the bioavailabilty of specific pollutants in complex environments, rather than the total concentration obtained by traditional analytical techniques. Here the focus is on the use of both natural and genetically engineered luminescent bacterial biosensors for the assessment of toxicity and bioavailability.

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Luminescent Bacterial Biosensors for the Rapid Detection of Toxicants

Davoren¹

2004

Water Encyclopedia

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Postexposure feeding depression: A new toxicity endpoint for use in laboratory studies with Daphnia magna

McWilliam

Baird

2002

Enviro Toxic and Chemistry

131

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In situ bioassays with daphnids currently employ lethality as an endpoint, and although sublethal responses (reproduction and feeding rate) can be measured in the field, such endpoints pose major practical challenges. Previous studies have indicated that Daphnia magna exposed to toxic substances can exhibit delayed recovery in feeding behavior (postexposure feeding depression). This simple, robust response has the potential to be an ecologically relevant and potentially diagnostic endpoint. This study developed and tested the use of postexposure feeding depression as a toxicity endpoint in the laboratory environment. First, replicate numbers were manipulated to produce statistically reliable results. Second, postexposure feeding depression in D. magna was studied under laboratory conditions, by employing toxic substances with differing modes of action. Although most substances caused feeding inhibition during direct exposure, not all substances produced postexposure feeding depression. However, the use of lethality as a supplementary endpoint provided an alternative measure when no feeding depression was apparent after exposure. In combination, these endpoints offer a potentially more sensitive, ecologically relevant alternative to the use of lethality alone for in situ bioassay studies.

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