Use of Buccal Cells Collected in Mouthwash as a Source of DNA for Clinical Testing

Heath, Ellen M.; Morken, Nathaniel W.; Campbell, Kristen A.; Tkach, Dennis; Boyd, Erin A.; Strom, Daniel A.

doi:10.5858/2001-125-0127-uobcci

Cited by 88 publications

(9 citation statements)

References 10 publications

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“…Our results suggest that samples collected by Scope mouthwash are generally stable for most microbial metrics, but there was higher abundance of the phylum Firmicutes and lower Proteobacteria after 4 days at room temperature. Similar to our results, samples collected with mouthwash in previous studies found microbial composition was stable for 4 days and 1–2 weeks variable lengths of time [ 7 , 22 , 23 ]. One study also found an increase in relative abundance of Firmicutes and a decrease in Bacteroidetes, Proteobacteria, and Fusobacteria after 4 days at room temperature with Scope mouthwash samples [ 7 ].…”

Section: Discussionsupporting

confidence: 92%

Comparison of fecal and oral collection methods for studies of the human microbiota in two Iranian cohorts

Hullings

Ghanbari

et al. 2021

BMC Microbiol

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Background To initiate fecal and oral collections in prospective cohort studies for microbial analyses, it is essential to understand how field conditions and geographic differences may impact microbial communities. This study aimed to investigate the impact of fecal and oral sample collection methods and room temperature storage on collection samples for studies of the human microbiota. Results We collected fecal and oral samples from participants in two Iranian cohorts located in rural Yazd (n = 46) and urban Gonbad (n = 38) and investigated room temperature stability over 4 days of fecal (RNAlater and fecal occult blood test [FOBT] cards) and comparability of fecal and oral (OMNIgene ORAL kits and Scope mouthwash) collection methods. We calculated interclass correlation coefficients (ICCs) based on 3 alpha and 4 beta diversity metrics and the relative abundance of 3 phyla. After 4 days at room temperature, fecal stability ICCs and ICCs for Scope mouthwash were generally high for all microbial metrics. Similarly, the fecal comparability ICCs for RNAlater and FOBT cards were high, ranging from 0.63 (95% CI: 0.46, 0.75) for the relative abundance of Firmicutes to 0.93 (95% CI: 0.89, 0.96) for unweighted Unifrac. Comparability ICCs for OMNIgene ORAL and Scope mouthwash were lower than fecal ICCs, ranging from 0.55 (95% CI: 0.36, 0.70) for the Shannon index to 0.79 (95% CI: 0.69, 0.86) for Bray-Curtis. Overall, RNAlater, FOBT cards and Scope mouthwash were stable up to 4 days at room temperature. Samples collected using FOBT cards were generally comparable to RNAlater while the OMNIgene ORAL were less similar to Scope mouthwash. Conclusions As microbiome measures for feces samples collected using RNAlater, FOBT cards and oral samples collected using Scope mouthwash were stable over four days at room temperature, these would be most appropriate for microbial analyses in these populations. However, one collection method should be consistently since each method may induce some differences.

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Section: Discussionsupporting

confidence: 92%

Comparison of fecal and oral collection methods for studies of the human microbiota in two Iranian cohorts

Hullings

Ghanbari

et al. 2021

BMC Microbiol

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“…DNA was collected in mouthwash (Heath et al, 2001) and extracted using the standard phenol-chloroform method. DNA concentrations were determined using a NanoDrop ND-1000 Spectrophotometer (ThermoFisher Scientific, Waltham, MA) and standardized to 10 ng/µL for the polymerase-chain-reaction (PCR) protocol.…”

Section: Genotyping Of Ar Cag Repeat Polymorphismmentioning

confidence: 99%

Using a Psychopharmacogenetic Approach To Identify the Pathways Through Which—and the People for Whom—Testosterone Promotes Aggression

et al. 2019

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Little is known about the neurobiological pathways through which testosterone promotes aggression or about the people in whom this effect is observed. Using a psychopharmacogenetic approach, we found that testosterone increases aggression in men ( N = 308) with select personality profiles and that these effects are further enhanced among those with fewer cytosine-adenine-guanine (CAG) repeats in exon 1 of the androgen receptor (AR) gene, a polymorphism associated with increased AR efficiency. Testosterone’s effects were rapid (~30 min after administration) and mediated, in part, by subjective reward associated with aggression. Testosterone thus appears to promote human aggression through an AR-related mechanism and to have stronger effects in men with the select personality profiles because it more strongly upregulates the subjective pleasure they derive from aggression. Given other evidence that testosterone regulates reward through dopaminergic pathways, and that the sensitivity of such pathways is enhanced among individuals with the personality profiles we identified, our findings may also implicate dopaminergic processes in testosterone’s heterogeneous effects on aggression.

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“…Various authors report that saliva samples obtained after the use of mouthwash can be stored at room temperature for 1-2 weeks without affecting the quality or quantity of DNA. 17,18,24,25 In the present study, we found that the samples stored at room temperature in Mexico City (20-24 8C) could be maintained stable and viable for up to 30 days. Although the DNA concentration was sharply reduced during this period, the quality and quantity of genomic DNA were still in optimal conditions for performing genotyping and sequencing.…”

Section: Discussionmentioning

confidence: 47%

Design of a protocol for obtaining genomic DNA from saliva using mouthwash: Samples taken from patients with periodontal disease

Mendoza

Buentello-Volante

Hernández

et al. 2016

Journal of Oral Biology and Craniofacial Research

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Use of Buccal Cells Collected in Mouthwash as a Source of DNA for Clinical Testing

Cited by 88 publications

References 10 publications

Comparison of fecal and oral collection methods for studies of the human microbiota in two Iranian cohorts

Comparison of fecal and oral collection methods for studies of the human microbiota in two Iranian cohorts

Using a Psychopharmacogenetic Approach To Identify the Pathways Through Which—and the People for Whom—Testosterone Promotes Aggression

Design of a protocol for obtaining genomic DNA from saliva using mouthwash: Samples taken from patients with periodontal disease

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