Use of Brucella abortus vaccine strain RB51 in pregnant cows after calfhood vaccination with strain 19 in Argentina

Samartino, Luis; Fort, Marcelo; Gregoret, R.; Schurig, Gerhardt G.

doi:10.1016/s0167-5877(00)00130-6

Cited by 30 publications

(28 citation statements)

References 9 publications

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“…Low numbers of positive reactions in rapid agglutination tests have been noticed after RB51 revaccination of adults previously vaccinated with S19 during calfhood but the same sera were negative in confirmatory test [89]. Some authors have reported that this revaccination induces antibodies detectable in tube serum agglutination and competitive ELISA in animals that had had persistent reactions in rapid agglutination tests [127] but others have not made this same observation [111]. Classical tests use suspensions of S brucellae where the LPS core (shared totally or partially by S and R brucellae) is not exposed and, therefore, the anti R-LPS antibodies elicited by RB51 are not detected.…”

Section: Interference In S-lps Testsmentioning

confidence: 99%

“…It is therefore possible that R vaccines show some degree of interference in these tests. It has been repeatedly reported that the full dose of RB51 in calves and the use of RB51 as a booster vaccine in adults that had been vaccinated with S19 during calfhood do not elicit antibodies detectable in the indirect ELISA [45,111,117]. However, we have observed that the indirect ELISA with S-LPS is positive in a significant proportion of RB51 vaccinated sheep and also in RB51 vaccinated cattle negative in the rose bengal test (Marín C.M., Blasco J.M., Moreno E., unpublished results), which suggests that attention should be paid to the standardization of the S-LPS ELISA when vaccination with RB51 is implemented.…”

Section: Interference In S-lps Testsmentioning

confidence: 99%

“…In a comparative study with a short number of animals (n = 5 in each group), neither RB51 nor S19 administered subcutaneously (9.4 and 3 × 10 8 CFU, respectively) caused abortions or placentitis lesions [95]. In two experiments with 57 and 13 strain 19 calfhoodvaccinated cattle, revaccination with the reduced dose of RB51 did not also cause abortion [111,127]. However, the booster vaccine (see Sect.…”

Section: Safetymentioning

confidence: 99%

“…In contrast, the differences between S19 vaccinated and non vaccinated calves remain highly significant under both the less (Experiment 3) and the most (Experiment 4) stringent challenges, and the differences between S19 and RB51 become more apparent in the most stringent experiment. Experiment 4 is cited in the literature as the demonstration that S19 and RB51 are similarly effective in protecting cattle [68,111,113] but without a description of the particular experimental conditions (i.e. the low % of infected controls) this presently seems an overstatement.…”

Section: Protection Against B Abortus In Controlled Experimentsmentioning

confidence: 99%

“…There are reports on RB51 use (reduced dose) after calfhood vaccination with S19 and data on the seroconversion in different tests (see Sect. 7.2.1) [89,111,127]. The degree of reinforcement of immunity achieved is not presently known.…”

Section: Rb51 As a Booster Vaccinementioning

confidence: 99%

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Rough vaccines in animal brucellosis: Structural and genetic basis and present status

Grilló²,

et al. 2004

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-Brucellosis control and eradication requires serological tests and vaccines. Effective classical vaccines (S19 in cattle and Rev 1 in small ruminants), however, induce antibodies to the O-polysaccharide of the lipopolysaccharide which may be difficult to distinguish from those resulting from infection and may thus complicate diagnosis. Rough attenuated mutants lack the O-polysaccharide and would solve this problem if eliciting protective immunity; the empirically obtained rough mutants 45/20 and RB51 have been used as vaccines. Strain 45/20 is reportedly unstable and it is not presently used. RB51 is increasingly used instead of S19 in some countries but it is rifampicin resistant and its effectiveness is controversial. Some controlled experiments have found good or absolute protection in adult cattle vaccinated orally (full dose) or subcutaneously (reduced dose) and in one field experiment, RB51 was reported to afford absolute protection to calves and to perform better than S19. Controlled experiments in calves, however, have shown reduced doses of RB51 to be ineffective, full doses only partially effective, and RB51 less effective than S19 against severe challenges. Moreover, other observations suggest that RB51 is ineffective when prevalence is high. RB51 is not useful in sheep and evidence in goats is preliminary and contradictory. Rough mutants obtained by molecular biology methods on the knowledge of the genetics and structure of Brucella lipopolysaccharide may offer alternatives. The B. abortus manB core (rfbK) mutant seems promising in cattle, and analyses in mice suggest that mutations affecting only the O-polysaccharide result in better vaccines than those affecting both core and O-polysaccharide. Possible uses of rough vaccines also include boosting immunity by revaccination but solid evidence on its effectiveness, safety and practicality is not available.

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Section: Interference In S-lps Testsmentioning

confidence: 99%

Section: Interference In S-lps Testsmentioning

confidence: 99%

Section: Safetymentioning

confidence: 99%

Section: Protection Against B Abortus In Controlled Experimentsmentioning

confidence: 99%

Section: Rb51 As a Booster Vaccinementioning

confidence: 99%

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