The Genus Brucella

Moreno, Edgardo; Moriyón, Ignacio

doi:10.1007/0-387-30745-1_17

Cited by 106 publications

(172 citation statements)

References 576 publications

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“…Zoonotic species such as Brucella melitensis, Brucella abortus, and Brucella suis are facultative extracellular-intracellular stealthy pathogens that are able to overcome innate immunity at early times of infection (1)(2)(3) and at specific stages of adaptive immunity (4,5). In addition to influencing the immune response, these Brucella species are able to circumvent the killing action of professional and nonprofessional phagocytes, transit within phagocytic vacuoles, and replicate extensively within the endoplasmic reticulum of cells (6).…”

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confidence: 99%

“…In addition to influencing the immune response, these Brucella species are able to circumvent the killing action of professional and nonprofessional phagocytes, transit within phagocytic vacuoles, and replicate extensively within the endoplasmic reticulum of cells (6). These properties allow the bacterium to spread throughout the reticuloendothelial system and promote chronic infection (3).…”

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Brucella canis Is an Intracellular Pathogen That Induces a Lower Proinflammatory Response than Smooth Zoonotic Counterparts

et al. 2015

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e Canine brucellosis caused by Brucella canis is a disease of dogs and a zoonotic risk. B. canis harbors most of the virulence determinants defined for the genus, but its pathogenic strategy remains unclear since it has not been demonstrated that this natural rough bacterium is an intracellular pathogen. Studies of B. canis outbreaks in kennel facilities indicated that infected dogs displaying clinical signs did not present hematological alterations. A virulent B. canis strain isolated from those outbreaks readily replicated in different organs of mice for a protracted period. However, the levels of tumor necrosis factor alpha, interleukin-6 (IL-6), and IL-12 in serum were close to background levels. Furthermore, B. canis induced lower levels of gamma interferon, less inflammation of the spleen, and a reduced number of granulomas in the liver in mice than did B. abortus. When the interaction of B. canis with cells was studied ex vivo, two patterns were observed, a predominant scattered cell-associated pattern of nonviable bacteria and an infrequent intracellular replicative pattern of viable bacteria in a perinuclear location. The second pattern, responsible for the increase in intracellular multiplication, was dependent on the type IV secretion system VirB and was seen only if the inoculum used for cell infections was in early exponential phase. Intracellular replicative B. canis followed an intracellular trafficking route undistinguishable from that of B. abortus. Although B. canis induces a lower proinflammatory response and has a stealthier replication cycle, it still displays the pathogenic properties of the genus and the ability to persist in infected organs based on the ability to multiply intracellularly. Brucellosis is a disease of animals and humans caused by members of the genus Brucella. Zoonotic species such as Brucella melitensis, Brucella abortus, and Brucella suis are facultative extracellular-intracellular stealthy pathogens that are able to overcome innate immunity at early times of infection (1-3) and at specific stages of adaptive immunity (4, 5). In addition to influencing the immune response, these Brucella species are able to circumvent the killing action of professional and nonprofessional phagocytes, transit within phagocytic vacuoles, and replicate extensively within the endoplasmic reticulum of cells (6). These properties allow the bacterium to spread throughout the reticuloendothelial system and promote chronic infection (3).There are other Brucella species that are also relevant pathogens; however, their infective strategies remain unclear and are not in tune with the solid results accepted for the previously mentioned zoonotic brucellae. Among these are Brucella canis, the etiological agent of brucellosis in dogs and a zoonotic pathogen (7). This pathogen induces a subclinical infection that may remain undiagnosed for protracted periods (8-10). B. canis invades the conjunctiva or the oronasal system or penetrates through the venereal route. Then it is distributed to different organs...

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Brucella canis Is an Intracellular Pathogen That Induces a Lower Proinflammatory Response than Smooth Zoonotic Counterparts

et al. 2015

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“…Brucelleae replicate within this vacuole and, if left untreated, eventually spread and cause a chronic infection of multiple tissues. Even after antibiotic treatment, the rate of relapse is high (6,7), and in a minority of patients, chronic brucellosis persists for years despite multiple courses of treatment (8).…”

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The Brucella abortus General Stress Response System Regulates Chronic Mammalian Infection and Is Controlled by Phosphorylation and Proteolysis

Kim

Caswell

Foreman

et al. 2013

Journal of Biological Chemistry

119

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Background: Virulence of pathogenic bacteria is often determined by their ability to adapt to stress. Results: The Brucella abortus general stress response (GSR) system is required for chronic mammalian infection and is regulated by phosphorylation and proteolysis. Conclusion: The B. abortus GSR signaling pathway has multiple layers of post-translational control and is a determinant of chronic infection. Significance: This study provides new, molecular level insight into chronic Brucella infection.

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“…were originally identified on the basis of their molecular weight (mol.wt.) (29). Group 1 was identified as minor (94 or 88 kDa).…”

Section: Praca Oryginalnamentioning

confidence: 99%

Using combined recombinant protein in the diagnosis of bovine brucellosis

Bulashev¹,

Jakubowski²,

Mukantayev³

et al. 2018

Medycyna Weterynaryjna

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SummaryThe aim of this research was to obtain a combined recombinant protein consisting of immunodominant regions of Brucella outer membrane proteins (Omps) with the molecular weights of 25 kDa (Omp25) and 31 kDa (Omp31). The search for candidate proteins was carried out using NCBI PubMed and NCBI GenBank databases. The two most immunodominant regions of Omps were selected. The first region was in a position from 48 to 83 amino acids of Omp31, while the second one was in the position from 180 to 224 amino acids of Omp25. This combined sequence was designated as OmpBm-Ba. The pET32 vector was used for cloning and the expression of the combined recombinant protein in E. coli BL21(DE3). The antigenicity of recombinant OmpBm-Ba (rOmpBm-Ba) as compared with rOmp25 and rOmp31 has been tested on the sera of 109 cattle with positive results to brucellosis according to classical serological tests. The rOmpBm-Ba had a higher antigenicity than the single ones, since it confirmed the presence of Brucella-antibody in all serum samples with positive results of i-ELISA/rOmp25 and/or i-ELISA/rOmp31, as well as additionally revealing 7.3% and 31.2% seropositive animals, respectively. A comparative study of the diagnostic value of i-ELISA/rOmpBm-Ba and conventional tests on blood sera of 24 cattle subjected to a bacteriological examination at slaughter showed a higher reliability of enzyme immunoassay. Further research is necessary to get a more objective evaluation of the diagnostic accuracy of Brucella rOmpBm-Ba by challenging laboratory animals.

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The Genus Brucella

Cited by 106 publications

References 576 publications

Brucella canis Is an Intracellular Pathogen That Induces a Lower Proinflammatory Response than Smooth Zoonotic Counterparts

Brucella canis Is an Intracellular Pathogen That Induces a Lower Proinflammatory Response than Smooth Zoonotic Counterparts

The Brucella abortus General Stress Response System Regulates Chronic Mammalian Infection and Is Controlled by Phosphorylation and Proteolysis

Using combined recombinant protein in the diagnosis of bovine brucellosis

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