Use of bromelain in cutaneous wound healing in streptozocin-induced diabetic rats: an experimental model

Fathi, Amene Nikgoftar; Sakhaie, Mohammad Hassan; Babaei, Sepehr; Babaei, Soroush; Slimabad, Fateme

doi:10.12968/jowc.2020.29.9.488

Cited by 14 publications

(7 citation statements)

References 42 publications

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“…The results of the in vitro assessment revealed that the bromelain extracted from macambira leaves enabled cell migration similar to that observed in the positive control, i.e., complete healing was achieved within 24 h. A previous study that assessed the in vivo healing activity of the pineapple peel protein extract reported no significant difference in the lesion histology results between the control and treatment groups [107]. Fathi et al (2020) [32] assessed the effect of bromelain on the various aspects of the wound healing process in type-1 diabetic rats. The authors reported that bromelain caused significant wound contraction and also reduced the formation of the granulation tissue by the 7 th day after treatment.…”

Section: Wound Healing Activity In Vitromentioning

confidence: 56%

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Biochemical Characteristics and Healing Activity of Bromelia laciniosa Leaf Protease

Silva¹,

Nerys²,

Silva³

et al. 2022

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Macambira plant (Bromelia laciniosa Mart. Ex Shult. & Schult. f.) is a species belonging to the family Bromeliaceae, which is distributed widely in the hinterlands of northeastern Brazil, from Bahia to Piauí states. The plants of this species contain an enzyme complex named bromelain, which has great potential for therapeutic applications. In the present study, bromelain derived from Bromelia laciniosa leaves was assessed for its thermodynamic properties and in vitro cytotoxic and healing activities. Bromelain was extracted from the leaves and partitioned using ethanol. The partially purified bromelain was then characterized to determine the optimum pH and temperature and the kinetic and thermodynamic parameters. The partially purified bromelain derived from macambira leaves exhibited its highest activity at 47°C and pH 7, when the Km and Vmax values of 2.26 µM and 2.083 U/mL, respectively. The half-life of this bromelain at 47°C was 5.78 h. At 47°C, the Gibbs's free energy, enthalpy, and entropy values reached 89, 53.54, and –0.11 kJ/mol K, respectively. In the SDS-PAGE analysis, bromelain appeared as the main protein band, with an estimated molecular mass of 33 kDa. The in vitro cytotoxic activity and wound healing effects were determined using the MTT assay and Scratch assay, respectively. These assays were performed on the non-neoplastic cell lines L-929 and PBMC and the human tumor cell lines MCF-7, HCT-116, and NCI-H292. Bromelain exhibited a positive healing activity with a change rate similar to that observed for the control. In addition, bromelain exhibited cytotoxic activity against NCI-H292 tumor cells and chemopreventive potential. These results indicate that bromelain derived from macambira leaves may be utilized as a potential ingredient in pharmaceutical formulations, for various healing and chemopreventive purposes and as an alternative to the bromelain extracted from pineapple. In addition, the biochemical characteristics of this bromelain could find application in the food industry. Future studies should, therefore, focus on deciphering the action mechanisms underlying the activities of bromelain observed in the present study to further confirm and enhance its application potential for therapeutic purposes.

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Section: Wound Healing Activity In Vitromentioning

confidence: 56%

“…Bromelain is also extensively used as an active ingredient in toothwhitening dentifrices and skincare products [29]. Bromelain may also be used for promoting and accelerating wound healing indices and improving the biomechanical parameters of diabetic wounds [30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Biochemical Characteristics and Healing Activity of Bromelia laciniosa Leaf Protease

Silva¹,

Nerys²,

Silva³

et al. 2022

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“…Bromelain, a complex mixture of proteolytic cysteine proteases obtained from stem of pineapple (Ananas comosus) is a food supplement exhibiting a variety of pharmacological activities. Various studies substantiate the bromelain's mucolytic [13], wound healing [14], fibrinolytic [15], antiedematous [16], antithrombotic [17], anti-inflammatory [18], antioxidant [19], anticancer [20] as well as immunomodulatory activity [21]. Bromelain significantly decreases COX-2 and PGE-2 expression and lower the cytokines (IL-1β, IL-6 and TNF-α) secretion from immune cells in inflammatory conditions [22,23].…”

Section: Introductionmentioning

confidence: 94%

Implications of nasal delivery of bromelain on its pharmacokinetics, tissue distribution and pharmacodynamic profile—A preclinical study

Sharma

Gupta²,

Pandey³

2022

PLoS ONE

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Asthma is a polygenic chronic inflammatory respiratory disease devastating the quality of life and state economies. Therefore, utilization of natural products as a therapeutic approach has attained wider consideration for development of novel drugs for asthma management. Bromelain, a mixture of natural bioactive cysteine proteases abundantly found in pineapple stem, has allured attention for its pharmacological activities. However, poor stability in gastric milieu, high dose and immunogenicity associated with prolonged use hinders its oral use. Therefore, need exists to explore alternative route of bromelain administration to achieve its plausible benefits. The present study investigated the preclinical prospects of nasal administration of bromelain on systemic bioavailability, tissue distribution and it’s in vivo anti-histaminic, bronchodilator and anti-asthmatic activity in animal models. Pharmacokinetic studies revealed 1.43-fold higher relative bioavailability with faster absorption of bromelain on nasal administration at one-fourth oral dose. The enhanced cellular uptake and localization of bromelain in tissues of lung was observed significantly. Furthermore, faster onset and enhanced antihistaminic, bronchodilator and anti-asthmatic activity on bromelain’s nasal administration signified faster absorption and higher in vivo stability of bromelain. Nasal administration significantly achieved decrease in level of oxidative and immunological markers along with restoration of antioxidant enzymes at considerably one-fourth dose administered orally. These findings distinctly manifested that nasal administration could be a substantial and effective route for bromelain delivery with enduring competency in asthma management.

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“…Their phytochemical content is the purpose of their remedial features in wound repair. Other phytochemicals and plant-derived substances were investigated for their wound-healing activity as flavonols, flavanones, isoflavones, flavanols, flavonolignans, proanthocyanidins (Carvalho et al 2021), β-glucans (Majtan and Jesenak 2018), bromelain (Fathi et al 2020), curcumin (Akbik et al 2014. It was disclosed that different botanicals and medicinal plants are widely used as a topical treatment for wound repairing, such as aloe vera, banana leaves (Sivamani et al 2012), turmeric, Centella asiatica, Rosmarinus officinalis, Calendula officinalis (Artem Ataide et al 2018).…”

Section: Introductionmentioning

confidence: 99%

The wound healing effect of botanicals and pure natural substances used in in vivo models

El-Sherbeni

Negm

2023

Inflammopharmacol

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Repairing the wound is a multistep process that includes the spatial and temporal synchronization of a different range of cell types to increase the speed of wound contraction, the proliferation of epithelial cells, and collagen formation. The need for proper management of acute wounds to be cured and not turned into chronic wounds is a significant clinical challenge. The traditional practice of medicinal plants in many regions of the world has been used in wound healing since ancient times. Recent scientific research introduced evidence of the efficacy of medicinal plants, their phyto-components, and the mechanisms underlying their wound-repairing activity. This review aims to briefly highlight the wound-curing effect of different plant extracts and purely natural substances in excision, incision, and burn experimental animal models with or without infection of mice, rats (diabetic and nondiabetic), and rabbits in the last 5 years. The in vivo studies represented reliable evidence of how powerful natural products are in healing wounds properly. They have good scavenging activity against Reactive oxygen species (ROS) and anti-inflammatory and antimicrobial effects that help in the process of wound healing. It is evident that incorporating bioactive natural products into wound dressings of bio- or synthetic polymers in nanofiber, hydrogel, film, scaffold, and sponge forms showed promising results in different phases of the wound-curing process of haemostasis, inflammation, growth, re-epithelialization, and remodelling.

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Use of bromelain in cutaneous wound healing in streptozocin-induced diabetic rats: an experimental model

Cited by 14 publications

References 42 publications

Biochemical Characteristics and Healing Activity of Bromelia laciniosa Leaf Protease

Biochemical Characteristics and Healing Activity of Bromelia laciniosa Leaf Protease

Implications of nasal delivery of bromelain on its pharmacokinetics, tissue distribution and pharmacodynamic profile—A preclinical study

The wound healing effect of botanicals and pure natural substances used in in vivo models

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