Use of Bao Gui capsule in treatment of a polycystic ovary syndrome rat model

Abstract

The present study was designed to elucidate the underlying mechanisms of Bao Gui capsule (BGc) against hyperandrogenism, insulin resistance and leptin resistance of PcoS. letrozole was used to induce a PcoS model in rats, which were then randomly divided into four groups (n=9): control, Model, high-dose BGc (BGc-H) and low-dose BGc (BGc-l) group. Serum levels of follicle-stimulating hormone (FSH), luteinizing hormone (lH), testosterone (T), estradiol (e 2 ), insulin, leptin, and interleukin (il)-1β, il-6 and tumor necrosis factor-α (TnF-α) in the hypothalamus were determined by eliSa. Protein levels of cytochrome P450c17α and cytochrome P450 aromatase (P450arom) in ovaries were determined by immunohistochemistry and western blot analysis. additionally, the expression of GluT4 in uterus and muscle tissue, and nF-κB, iKKβ and SocS3 mrna levels in the hypothalamus were evaluated. BGC significantly reduced body weight gain and decreased serum levels of lH/FSH, T, log T/e 2 , insulin and leptin compared with the PcoS model rats. Furthermore, BGc markedly reduced the expression of P450c17α and significantly increased the expression of P450arom in ovaries, and increased the expression of GluT4 in uterus and muscle tissues. BGc also effectively reduced the level of il-6 and TnF-α, and the expression of iKKβ, nF-κB and SocS3 in the hypothalamus of PcoS model rats. These results suggest that BGc may effectively improve hyperandrogenism, insulin resistance, endometrial receptivity and the low-grade chronic inflammation in the hypothalamus.

Effects of natural products on polycystic ovary syndrome: From traditional medicine to modern drug discovery

“…2 . Catechins, Sahoyao-gancao decoction, Bao Gui capsule, Cangfu Daotan decoction and nanocurcumin inhibited NF-ΚB mediated inflammation by decreasing the expression of phosphorylated NF-kappa-B p65 (p–NF–κB p65), IL-1α, IL-6, and TNF- α [ 16 , 38 , 39 , 51 , 69 ]. Moreover, studies found that Resveratrol, Berberis (B.)…”

“…The hydroalcoholic extract of flaxseed was effective in serum hormone levels and polycystic ovarian morphology [ 37 ]. Bao Gui capsule (BGC) attenuated hyperandrogenism via increasing Cyp19a1 and down-regulating P450c17 [ 38 ]. It also ameliorated insulin resistance and detracted some inflammatory markers and IKK /NF- signaling.…”

The Efficacy of Chinese Herbal Medicine in Animal Models of Polycystic Ovary Syndrome: A Systematic Review and Meta-Analysis

“…SGD might reduce the phosphorylation of nuclear factor kappa-B (NF-kB) p65, suppress toll like receptor 4 (TLR4)/NF-kB signaling pathway, remodel gut microbiome structure, and protect gut barrier, which leads to ameliorate the inflammatory response in the ovary of PCOS rats [ 25 , 33 ]. As the most effective formula to reduce high testosterone, BGC decreased the expression of P450c17 α and increased the expression of P450arom to improve hyperandrogenism [ 30 ]. There were also studies on the effect of CHM on ovarian granule cell apoptosis through the PKR-like Reticulum Kinase (PERK)-ATK4- C/EBP homologous protein (CHOP) signaling pathway, PI3K/AKT/mTOR pathway, and P13K/AKT/Forkhead Box Protein O1A (FOXO1A) pathway [ 15 , 23 , 24 , 31 ].…”

Research progress of endometrial receptivity in patients with polycystic ovary syndrome: a systematic review

“…IL-6 expression was found to increase in a mouse model of PCOS. It mediates chronic low-grade inflammation of the endometrium and produces various metabolic disorders, thereby reducing endometrial receptivity [ 48 ].…”