Use of Autologous Serum in the Treatment of Ocular Surface Disorders

Establishment of a novel in vitro model of stratified epithelial wound healing with barrier function

“…In our experiments, wounding of the stratified epithelium with a punch initiated a migratory phase that was dependent on the culture media employed. We found that medium with serum was crucial to promote re-epithelialization in the stratified cultures, in agreement with previous findings reporting successful use of autologous serum in promoting healing during the clinical management of persistent corneal epithelial defects 25 26 . Further, migration was associated with the presence of active proliferative cells within the concentric incision groove around the punch wound, in accordance with previous data showing an abundance of proliferative corneal epithelial cells in micro-ridges of engineered constructs 27 .…”

Establishment of a novel in vitro model of stratified epithelial wound healing with barrier function

“…In our experiments, wounding of the stratified epithelium with a punch initiated a migratory phase that was dependent on the culture media employed. We found that medium with serum was crucial to promote re-epithelialization in the stratified cultures, in agreement with previous findings reporting successful use of autologous serum in promoting healing during the clinical management of persistent corneal epithelial defects 25 26 . Further, migration was associated with the presence of active proliferative cells within the concentric incision groove around the punch wound, in accordance with previous data showing an abundance of proliferative corneal epithelial cells in micro-ridges of engineered constructs 27 .…”

Treatment of persistent epithelial defects with single-dose autologous serum eye drops

“…In the literature, different methods in various steps have been described for preparing ASEDs including serum amount, clotting time, centrifugation time and force, dilution rate, and diluents (Table 4). 1,6,19,21,31,37,38…”

“…27,35,36 In the literature, different methods in various steps have been described for preparing ASEDs including serum amount, clotting time, centrifugation time and force, dilution rate, and diluents (Table 4). 1,6,19,21,31,37,38 Serum can be stored for 7 days at 4°C and for 3-6 months at −20°C without decreasing the concentrations of vitamins and growth factors, while concentrations of these substances are more variable at room temperature. It has been reported that when the waiting time before centrifugation is long, the growth factor concentration becomes higher.…”

“…The volume of ASEDs prepared in the literature was between 1.5 and 10 ml at 20%-100% concentrations, and it was recommended that ASEDs be discarded after daily use. 6,[19][20][21][22]31,37,38 However, this requires repeated phlebotomies and increases the cost of serum preparation and the work-load of healthcare personnel.…”

Therapeutic outcomes of combined topical autologous serum eye drops with silicone–hydrogel soft contact lenses in the treatment of corneal persistent epithelial defects: A preliminary study

“…The first application of ASEs for the treatment of ocular disease can be traced back to at least 1975 [7], and the first documented successful treatment in patients was for keratoconjunctivitis sicca in 1984 [1,15]. Over the years, topical autologous serum has been used clinically as a topical in vivo medication for treating ocular surface disorders, such as recurrent corneal erosions [16], persistent epithelial defects [8,9,17,18], superior limbic keratoconjunctivitis [11], neurotrophic keratopathy [14], and dry eyes [12,13].…”

Establishment of a novel in vitro model of stratified epithelial wound healing with barrier function

“…In our experiments, wounding of the stratified epithelium with a punch initiated a migratory phase that was dependent on the culture media employed. We found that medium with serum was crucial to promote re-epithelialization in the stratified cultures, in agreement with previous findings reporting successful use of autologous serum in promoting healing during the clinical management of persistent corneal epithelial defects 25 26 . Further, migration was associated with the presence of active proliferative cells within the concentric incision groove around the punch wound, in accordance with previous data showing an abundance of proliferative corneal epithelial cells in micro-ridges of engineered constructs 27 .…”

Treatment of persistent epithelial defects with single-dose autologous serum eye drops

“…In the literature, different methods in various steps have been described for preparing ASEDs including serum amount, clotting time, centrifugation time and force, dilution rate, and diluents (Table 4). 1,6,19,21,31,37,38…”

“…27,35,36 In the literature, different methods in various steps have been described for preparing ASEDs including serum amount, clotting time, centrifugation time and force, dilution rate, and diluents (Table 4). 1,6,19,21,31,37,38 Serum can be stored for 7 days at 4°C and for 3-6 months at −20°C without decreasing the concentrations of vitamins and growth factors, while concentrations of these substances are more variable at room temperature. It has been reported that when the waiting time before centrifugation is long, the growth factor concentration becomes higher.…”

“…The volume of ASEDs prepared in the literature was between 1.5 and 10 ml at 20%-100% concentrations, and it was recommended that ASEDs be discarded after daily use. 6,[19][20][21][22]31,37,38 However, this requires repeated phlebotomies and increases the cost of serum preparation and the work-load of healthcare personnel.…”

Therapeutic outcomes of combined topical autologous serum eye drops with silicone–hydrogel soft contact lenses in the treatment of corneal persistent epithelial defects: A preliminary study

“…The first application of ASEs for the treatment of ocular disease can be traced back to at least 1975 [7], and the first documented successful treatment in patients was for keratoconjunctivitis sicca in 1984 [1,15]. Over the years, topical autologous serum has been used clinically as a topical in vivo medication for treating ocular surface disorders, such as recurrent corneal erosions [16], persistent epithelial defects [8,9,17,18], superior limbic keratoconjunctivitis [11], neurotrophic keratopathy [14], and dry eyes [12,13].…”

Establishment of a novel in vitro model of stratified epithelial wound healing with barrier function

“…In our experiments, wounding of the stratified epithelium with a punch initiated a migratory phase that was dependent on the culture media employed. We found that medium with serum was crucial to promote re-epithelialization in the stratified cultures, in agreement with previous findings reporting successful use of autologous serum in promoting healing during the clinical management of persistent corneal epithelial defects 25 26 . Further, migration was associated with the presence of active proliferative cells within the concentric incision groove around the punch wound, in accordance with previous data showing an abundance of proliferative corneal epithelial cells in micro-ridges of engineered constructs 27 .…”

Treatment of persistent epithelial defects with single-dose autologous serum eye drops

“…In the literature, different methods in various steps have been described for preparing ASEDs including serum amount, clotting time, centrifugation time and force, dilution rate, and diluents (Table 4). 1,6,19,21,31,37,38…”

“…27,35,36 In the literature, different methods in various steps have been described for preparing ASEDs including serum amount, clotting time, centrifugation time and force, dilution rate, and diluents (Table 4). 1,6,19,21,31,37,38 Serum can be stored for 7 days at 4°C and for 3-6 months at −20°C without decreasing the concentrations of vitamins and growth factors, while concentrations of these substances are more variable at room temperature. It has been reported that when the waiting time before centrifugation is long, the growth factor concentration becomes higher.…”

“…The volume of ASEDs prepared in the literature was between 1.5 and 10 ml at 20%-100% concentrations, and it was recommended that ASEDs be discarded after daily use. 6,[19][20][21][22]31,37,38 However, this requires repeated phlebotomies and increases the cost of serum preparation and the work-load of healthcare personnel.…”

Therapeutic outcomes of combined topical autologous serum eye drops with silicone–hydrogel soft contact lenses in the treatment of corneal persistent epithelial defects: A preliminary study

“…The first application of ASEs for the treatment of ocular disease can be traced back to at least 1975 [7], and the first documented successful treatment in patients was for keratoconjunctivitis sicca in 1984 [1,15]. Over the years, topical autologous serum has been used clinically as a topical in vivo medication for treating ocular surface disorders, such as recurrent corneal erosions [16], persistent epithelial defects [8,9,17,18], superior limbic keratoconjunctivitis [11], neurotrophic keratopathy [14], and dry eyes [12,13].…”