Use of atomic force microscopy as a diagnostic tool to identify orthopoxvirus

Trindade, Giliane S.; Vilela, José Mário Carneiro; Ferreira, Jaqueline Maria Siqueira; Aguiar, Pedro Henrique Nascimento; Leite, Juliana Almeida; Guedes, M.I.M.C.; Lobato, Zélia Inês Portela; Madureira, Marieta C.; Silva, M.I.N. da; Fonseca, Flávio Guimarães da; Kroon, Erna Geessien; Andrade, Margareth Spangler

doi:10.1016/j.jviromet.2006.12.006

Cited by 20 publications

(14 citation statements)

References 24 publications

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“…To avoid any possibility of field cross-contamination, each sample was collected and manipulated separately. In parallel, to confirm VACV infection, serum samples and scabs were collected from the same animals according to Trindade et al (2007) and submitted to plaque reduction neutralizing assays (Abdalrhman et al, 2006) and virus detection, respectively. A milk sample from a bulk tank was also analyzed.…”

Section: Collection and Preparation Of Bv Clinical Samplesmentioning

confidence: 99%

“…All prepared clinical specimens were submitted to semi-nested PCR for virus growth factor (vgf ) gene amplification without undergoing prior DNA extraction. The vgf gene is a conserved OPV gene, widely used as a PCR target, in association with other genes, in diagnostic and phylogenetic analysis of BV outbreaks (Trindade et al, 2007). An external primer was designed (Abrahã o et al, unpublished data) and used in association with VGF-F (5 0 CGCAGGATCCATAA TCAGTCATT3 0 ) and VGF-R (5 0 ACAATGGATATTTACG AC3 0 ) primers (Fonseca et al, 1998).…”

Section: Semi-nested Pcr For the Virus Growth Factor Genementioning

confidence: 99%

“…DNA was quantified (NanoDrop ND-1000; Thermo Scientific, Wilmington, DE) and submitted to PCR using timidine kinase (tk) primers (Fonseca et al, 1998). tk is a conserved gene in the OPV genus and has previously been used as a taxonomic tool to study ungrouped poxviruses (reviewed by Trindade et al, 2007). A PCR tk product from a sample of each outbreak was purified using the QIAquick Gel Extraction Kit (Qiagen, Duesseldorf, Germany) and cloned into a pGEM T-easy vector (Promega, Madison, WI).…”

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confidence: 99%

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Bovine Vaccinia Outbreaks: Detection and Isolation of Vaccinia Virus in Milk Samples

Abrahão

Oliveira

Campos

et al. 2009

Foodborne Pathogens and Disease

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The vaccinia virus (VACV), which causes exanthemous lesions in dairy cattle and humans, has been associated with several bovine vaccinia outbreaks in Brazil. Currently, no data are available about the safety of milk produced in VACV-affected areas. In this study, 47 milk samples were collected during bovine vaccinia outbreaks and submitted to viral isolation, DNA detection, and nucleotide sequencing of the conserved tk gene. The appearance of characteristic white pocks on the chorioallantoic membranes of chicken eggs, in association with viral cytopathic effects in chicken embryo fibroblasts and phylogenetic data, strongly suggest milk contamination by VACV. This is the first report of VACV detection in and isolation from milk.

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Section: Collection and Preparation Of Bv Clinical Samplesmentioning

confidence: 99%

Section: Semi-nested Pcr For the Virus Growth Factor Genementioning

confidence: 99%

mentioning

confidence: 99%

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Bovine Vaccinia Outbreaks: Detection and Isolation of Vaccinia Virus in Milk Samples

Abrahão

Oliveira

Campos

et al. 2009

Foodborne Pathogens and Disease

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“…Both options are seriously considered, however the former has the advantage of simplicity. Since viral capsids have well-defined shapes, it is relatively easy to detect and classify them by AFM in very small amounts of crude samples, including the surface of fixed cells [58,144,145]. The VIPER database of icosahedral capsids (http://viperdb.scripps.edu) provides the X-ray crystallography and cryoEM data on several hundreds of structures, which should be useful to guide interpretation of AFM data [146].…”

Section: Afm Of Protein Complexes In Nanomedicine and Nanobio Techmentioning

confidence: 99%

“…The VIPER database of icosahedral capsids (http://viperdb.scripps.edu) provides the X-ray crystallography and cryoEM data on several hundreds of structures, which should be useful to guide interpretation of AFM data [146]. Moreover, high-quality AFM topography images of more than a dozen of pathological viruses were published to date [51,144,145,147]. Automated image analysis is very helpful in extracting the structural information, as was shown for rotaviruses [148].…”

Section: Afm Of Protein Complexes In Nanomedicine and Nanobio Techmentioning

confidence: 99%

AFM of biological complexes: What can we learn?

Gaczyńska

Osmulski

2008

Current Opinion in Colloid & Interface Science

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The term “biological complexes” broadly encompasses particles as diverse as multisubunit enzymes, viral capsids, transport cages, molecular nets, ribosomes, nucleosomes, biological membrane components and amyloids. The complexes represent a broad range of stability and composition. Atomic force microscopy offers a wealth of structural and functional data about such assemblies. For this review, we choose to comment on the significance of AFM to study various aspects of biology of selected nonmembrane protein assemblies. Such particles are large enough to reveal many structural details under the AFM probe. Importantly, the specific advantages of the method allow for gathering dynamic information about their formation, stability or allosteric structural changes critical for their function. Some of them have already found their way to nanomedical or nanotechnological applications. Here we present examples of studies where the AFM provided pioneering information about the biology of complexes, and examples of studies where the simplicity of the method is used toward the development of potential diagnostic applications.

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Impedimetric Immunosensor for Detection of Plum Pox Virus in Plant Extracts

et al. 2011

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This paper concerns the development of an immunosensor for detection of Plum pox virus in plant extracts. The sensor was based on gold electrodes modified with: 1,6-hexanedithiol, gold nanoparticles, anti-PPV IgG polyclonal antibody and BSA. It was used for determination of the virus in extracts from plum (Prunus domestica) and tobacco (Nicotiana benthamiana) leaves. The proposed electrochemical impedance spectroscopy (EIS) immunosensor displayed a very good detection limit of 10 pg/mL and a wide dynamic range from 10 pg PPV/ mL to 200 pg/mL. The presence of extracts from plant materials has no influence on the immunosensors response. The immunosensor was capable of discriminating between samples from healthy plants and samples containing 0.01 % of extract from infected plant material. The conditions for immunosensor regeneration are also presented.

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Use of atomic force microscopy as a diagnostic tool to identify orthopoxvirus

Cited by 20 publications

References 24 publications

Bovine Vaccinia Outbreaks: Detection and Isolation of Vaccinia Virus in Milk Samples

Bovine Vaccinia Outbreaks: Detection and Isolation of Vaccinia Virus in Milk Samples

AFM of biological complexes: What can we learn?

Impedimetric Immunosensor for Detection of Plum Pox Virus in Plant Extracts

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