Impedimetric Immunosensor for Detection of <i>Plum Pox Virus</i> in Plant Extracts

Jarocka, Urszula; Wąsowicz, Michał; Radecka, Hanna; Malinowski, Tadeusz; Michalczuk, Lech; Radecki, Jerzy

doi:10.1002/elan.201100152

Cited by 51 publications

(31 citation statements)

References 33 publications

(35 reference statements)

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“…One of the most recent technique is impedimetric immunosensor, which is based on gold electrodes modified with 1,6-hexanedithiol, gold nanoparticles, anti-PPV IgG polyclonal antibody and bovine serum albumin. The proposed technique displays very good detection limit (10 pg/ml) and is able to differentiate the samples from healthy plants and the samples containing 0.01 % of infected plant extract [378]. The PCR-ELISA, respectively RT-PCR-ELISA, which is based on immunoenzymatic detection of PCR products, represents next possibility of ELISA/PCR modification with significant increase of sensitivity compared to both ELISA and PCR methods [374,379,380].…”

Section: Detection Of Ppvmentioning

confidence: 99%

Sharka: The Past, The Present and The Future

Sochor

Babula

Adam

et al. 2012

Viruses

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Members the Potyviridae family belong to a group of plant viruses that are causing devastating plant diseases with a significant impact on agronomy and economics. Plum pox virus (PPV), as a causative agent of sharka disease, is widely discussed. The understanding of the molecular biology of potyviruses including PPV and the function of individual proteins as products of genome expression are quite necessary for the proposal the new antiviral strategies. This review brings to view the members of Potyviridae family with respect to plum pox virus. The genome of potyviruses is discussed with respect to protein products of its expression and their function. Plum pox virus distribution, genome organization, transmission and biochemical changes in infected plants are introduced. In addition, techniques used in PPV detection are accentuated and discussed, especially with respect to new modern techniques of nucleic acids isolation, based on the nanotechnological approach. Finally, perspectives on the future of possibilities for nanotechnology application in PPV determination/identification are outlined.

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Section: Detection Of Ppvmentioning

confidence: 99%

Sharka: The Past, The Present and The Future

Sochor

Babula

Adam

et al. 2012

Viruses

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“…The immunosensor presented displayed better detection limit in the comparison to those already prepared in our laboratory (Table 1) (Jarocka et al, 2013(Jarocka et al, , 2011. The main reason for this could be the different way of immobilization procedures of sensing elements on the electrode surface.…”

Section: Detection Of the Anti Hemagglutinin Antibody In Hen Seramentioning

confidence: 73%

“…Thus, the antibodies orientation on the electrode surface is rather random (Jarocka et al, 2011). For recognitions of Prunus Necrotic Ringspot Virus (PNRV), the antibodies were attached to via protein A.…”

Section: Detection Of the Anti Hemagglutinin Antibody In Hen Seramentioning

confidence: 99%

Electrochemical immunosensor for detection of antibodies against influenza A virus H5N1 in hen serum

Jarocka

Sawicka

Góra-Sochacka

et al. 2014

Biosensors and Bioelectronics

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This paper describes the development of an immunosensor for detection of anti-hemagglutinin antibodies. Its preparation consists of successive modification steps of glassy carbon electrodes: (i) creation of COOH groups, (ii) covalent immobilization of protein A with EDC/NHS coupling reaction, (iii) covering with anti-His IgG monoclonal antibody, (iv) immobilization of the recombinant His-tagged hemagglutinin (His6-H5 HA), (v) filling free space with BSA. The interactions between two variants of recombinant HA (short and long) from highly pathogenic avian influenza virus H5N1 and the anti-H5 HA monoclonal antibody (Mab 6-9-1) have been explored with electrochemical impedance spectroscopy (EIS). The impedimetric immunosensor displayed a very good detection limit (LOD) of 2.1 pg/mL, the quantification limit (LOQ) of 6.3 pg/mL and a dynamic range from 4 pg/mL to 20 pg/mL. In addition, this analytical device was applied for detection of antibodies against His6-H5 HA in serum of vaccinated hen using serial 10-fold dilutions of serum. The immunosensor proposed was able to detect antibody in hen serum diluted up to 7 x 10 7 -fold.The sensitivity of immunosensor was about four orders of magnitude much better than ELISA.

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“…The most popular mode for analyzing electrochemical impedance data is the plot of the imaginary impedance component versus real impedance component at each excitation frequency the Nyquist plot . Numerous impedimetric immunosensors have been already reported [7,30,31,38]. This approach has been successfully applied for detection of fragments of H5 hemagglutinin from avian inluenza viruses [9,10].…”

Section: Methods Of Detection Of Immunoreaction: Selected Examplementioning

confidence: 99%

Electrochemical Sensors for Detections of Influenza Viruses: Fundamentals and Applications

Radecka¹,

Radecki²

2016

Steps Forwards in Diagnosing and Controlling Influenza

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Abstract"vian inluenza viruses "IVs could cause severe diseases and, as the consequence, serious economic losses as well as a risk for potential transmission to humans. Therefore, the detection of viruses and their fragments or speciic DN" sequences becomes an important approach in molecular diagnosis. Here, we present electrochemical genosensors devoted for detection of inluenza virus H5N1 gene sequence. We focus our atention on ion-channel mechanism, E-DN" sensors, and genosensors based on redox-active layer. The novel dual DN" electrochemical sensor with "signal-of" and "signal-on" architecture for simultaneous detection of two diferent sequences of DN" derived from avian inluenza virus type H5N1 by means of one electrode is presented. Immunosensors are also adequate analytical devices for detection of pathogens since antibodies are natural receptors responsible for binding of antigens. Thus, the binding selectivity and eiciency are naturally high. The immunosensors presented could be divided into two main groups: ion-channel mimetic and based on redox-active monolayer.

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Impedimetric Immunosensor for Detection of Plum Pox Virus in Plant Extracts

Cited by 51 publications

References 33 publications

Sharka: The Past, The Present and The Future

Sharka: The Past, The Present and The Future

Electrochemical immunosensor for detection of antibodies against influenza A virus H5N1 in hen serum

Electrochemical Sensors for Detections of Influenza Viruses: Fundamentals and Applications

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