Use of antioxidants to augment semen efficiency during liquid storage and cryopreservation in livestock animals: A review

Al-Mutary, Mohsen Ghaleb

doi:10.1016/j.jksus.2020.10.023

Cited by 24 publications

(16 citation statements)

References 59 publications

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“…The reason for this is that the plasma membranes of spermatozoa contain high levels of unsaturated fatty acids and lipid peroxidation due to reactive oxygen species (ROS) (Özer Kaya et al, 2018). Besides, although enzymatic and non‐enzymatic antioxidants are essential compounds of the seminal plasma, their protection against oxidative events decrease due to rebuilding procedures before storage (Al‐Mutary, 2021; Peris‐Frau et al, 2020). For these reasons, semen preservation studies focus on natural antioxidants.…”

Section: Introductionmentioning

confidence: 99%

Thymoquinone improves motility, plasma membrane integrity and DNA integrity of frozen–thawed ram semen

et al. 2022

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The aim of this study was to determine the effects of thymoquinone (TQ), which is the most essential active compound of Nigella sativa, on the spermatological parameters of ram semen during cryopreservation. Ejaculates were collected from five Sonmez rams using an artificial vagina and extended with Tris‐based extender not containing TQ (control, 0 μg/ml TQ) and containing 10, 25, 50 and 100 μg/ml TQ. The extended semen samples were equilibrated in a + 4°C cold cabinet for 2 h. After 2 h, the samples were loaded into 0.25 ml French straws. The straws were frozen by liquid nitrogen vapour and stored in a liquid nitrogen container (−196°C). The frozen straws were thawed in a water bath (37°C for 30 s) and evaluated in terms of motility characteristics, plasma membrane and acrosome integrity, mitochondrial reactive oxygen species levels, lipid peroxidation levels, DNA damage and biochemical alterations (oxidative stress index, malondialdehyde and glutathione). TQ100 had higher total motility (53.59 ± 3.01) and progressive motility (19.84 ± 1.44; not significantly different from TQ25 and TQ50) compared to the control and TQ10 (p ˂ 0.05). According to the results of the analyses on motility characteristics, there were significant differences between the groups in terms of curvilinear velocity (VCL), amplitude of lateral head displacement (ALH) and linearity (LIN; p ˂ 0.05). The highest DNA damage was detected in the control group (p ˂ 0.05). TQ50 had higher plasma membrane and acrosome integrity (59.56 ± 5.92) compared to the control and TQ25 (p < 0.05) but not significantly different from TQ10 and TQ100. The lowest mitochondrial reactive oxygen species levels were detected in TQ50 and TQ100 (p ˂ 0.05). There were no significant differences among the groups in terms of their oxidative stress index, lipid peroxidation, malondialdehyde and glutathione levels (p > 0.05). According to the results, it could be concluded that supplementing 50 or 100 μg/ml TQ to Tris extenders that were used for ram semen cryopreservation showed a positive effect on motility, plasma membrane integrity and acrosome integrity, and it reduced DNA damage and mitochondrial reactive oxygen species levels.

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Section: Introductionmentioning

confidence: 99%

Thymoquinone improves motility, plasma membrane integrity and DNA integrity of frozen–thawed ram semen

et al. 2022

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“…In several studies, researchers have reduced ROS levels in sperm using antioxidants by increasing the ability to scavenge free radicals. Therefore, adding antioxidants to semen freezing media is one of the ways to reduce stress oxidation during the freezing and thawing process, and much research has been done to improve the quality of freezing media by enriching them with various antioxidants (Al‐Mutary, 2021).…”

Section: Discussionmentioning

confidence: 99%

Effect of Myo‐inositol on sperm quality and biochemical factors in cryopreserved semen of patients with Asthenospermia

2022

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In this study, the influence of myoinositol (MYO) as an antioxidant on the inhibition of the negative impacts of cryopreservation on sperm quality in men with Asthenospermia was investigated. In this prospective study, each semen sample from 25 cases was separated into three groups: Fresh, Control (with freezing medium), Myoinositol (2 mg/ml). According to the World Health Organization criteria (WHO) (2010), total motility, progressive sperm motility, viability, normal morphology, and DNA integrity were assessed. In addition, the hypo‐osmotic swelling (HOS) test and mitochondrial membrane potential (MMP) were used. Total antioxidant capacity (TAC), malondialdehyde (MDA), and antioxidant enzyme activity were determined by the ELISA method. In contrast to the fresh samples, lipid peroxidation, DNA integrity damage, DNA fragmentation, HOST, and MMP had significant enhancement in the control samples. Sperm quality was significantly decreased (p < 0.05). Mean percentage viability, normal morphology, total motility, progressive motility, and DNA integrity were significantly enhanced in the MYO group in comparison to the control group (p < 0.05). The MDA and TAC levels and DNA damage in the MYO group were significantly lower compared to the control group (p < 0.05). The findings confirm that sperm quality in patients with Asthenospermia is improved by the administration of 2 mg/ml of myoinositol together with the freezing medium after sperm cryopreservation.

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“…Prediction of reactive oxygen species and peroxidation can also be controlled by adding substances similar to antioxidants in freezing media [ 116 ]. Optimum levels of ROS are inevitable for oocyte sperm fertilization and acrosome reactions, but as these levels exceed, the functionality of the sperm is disrupted [ 117 ].…”

Section: Addition Of Seminal Plasma Components and Other Supplementsmentioning

confidence: 99%

Effect of Sperm Cryopreservation in Farm Animals Using Nanotechnology

Akhtar

et al. 2022

Animals

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Sperm cryopreservation is one of the sublime biotechnologies for assisted reproduction. In recent decades, there has been an increasing trend in the use of preserved semen. Post-thaw semen quality and values vary among animals of the same species. Similarly, there are species-specific variations in sperm morphology, i.e., sperm head, kinetic properties, plasma membrane integrity, and freezability. Similarly, the viability of sperm varies in the female reproductive tract, i.e., from a few hours (in cattle) to several days (in chicken). Various steps of sperm cryopreservation, i.e., male health examination, semen collection, dilution, semen centrifugation, pre- and post-thaw semen quality evaluation, lack standardized methodology, that result in differences in opinions. Assisted reproductive technologies (ART), including sperm preservation, are not applied to the same extent in commercial poultry species as in mammalian species for management and economic reasons. Sperm preservation requires a reduction in physiological metabolism by extending the viable duration of the gametes. Physiologically and morphologically, spermatozoa are unique in structure and function to deliver paternal DNA and activate oocytes after fertilization. Variations in semen and sperm composition account for better handling of semen, which can aid in improved fertility. This review aims to provide an update on sperm cryopreservation in farm animals.

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Use of antioxidants to augment semen efficiency during liquid storage and cryopreservation in livestock animals: A review

Cited by 24 publications

References 59 publications

Thymoquinone improves motility, plasma membrane integrity and DNA integrity of frozen–thawed ram semen

Thymoquinone improves motility, plasma membrane integrity and DNA integrity of frozen–thawed ram semen

Effect of Myo‐inositol on sperm quality and biochemical factors in cryopreserved semen of patients with Asthenospermia

Effect of Sperm Cryopreservation in Farm Animals Using Nanotechnology

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