Use of antioxidants reduce lipid peroxidation and improve quality of crossbred ram sperm during its cryopreservation

Banday, Mohamad Naiem; Lone, Farooz Ahmad; Rasool, Farahnaz; Rashid, Muzamil; Shikari, Arif

doi:10.1016/j.cryobiol.2016.12.008

Cited by 85 publications

(65 citation statements)

References 52 publications

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“…Therefore, the main mechanism for disrupting the integrity of DNA and chromatin is the increase of ROS and oxidative stress (Cabrita et al, ; Sabour et al, ). It seems that the antioxidant capacity of the sperm cell is inadequate to overcome the damage caused by the ROS formed during the freezing‐thawing process (Banday, Lone, Rasool, Rashid, & Shikari, ). Antioxidants play an important role in supporting sperm and can neutralise the effects induced by the freezing process (Cabrita et al, ).…”

Section: Discussionmentioning

confidence: 99%

Canthaxanthin protects human sperm parameters during cryopreservation

2019

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Different antioxidants have been introduced to reduce oxidative stress during the cryopreservation. The main goal of this study was to evaluate the effects of canthaxanthin on human sperm parameters during the freeze‐thaw process. This study was performed on 25 normozoospermic semen samples dividing into five groups including 0, 0.1, 1, 10, and 25 µM of canthaxanthin. The prepared spermatozoa were cryopreserved by rapid freezing technique. Sperm motility, viability (eosin‐nigrosin), morphology (Papanicolaou), acrosome reaction (double staining), DNA denaturation (acridine orange), chromatin packaging (aniline blue and toluidine blue), and DNA fragmentation (sperm chromatin dispersion test) were evaluated before freezing and after thawing. All sperm parameters after thawing significantly were decreased compared to before freezing. Twenty‐five micromolar canthaxanthin could significantly improve the progressive and total motility, viability, normal morphology, chromatin packaging, acrosome integrity and DNA denaturation and fragmentation. Ten micromolar canthaxanthin significantly improved total motility, viability, normal morphology, chromatin packaging, acrosome integrity and DNA denaturation and fragmentation. Whereas, in 1 µM group, there were significant differences only in improvement of acrosome integrity, chromatin packaging (toluidine blue) and DNA denaturation and fragmentation. But, in 0.1 µM group, there were no significant differences in any of measured parameters. It seems that canthaxanthin ameliorates detrimental effects of cryopreservation on human sperm parameters.

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Section: Discussionmentioning

confidence: 99%

Canthaxanthin protects human sperm parameters during cryopreservation

2019

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“…Ram spermatozoa are exposed to extreme oxidative stress during cryopreservation, due to an insufficient and unbalanced antioxidant defence system that cannot mitigate effects of production of excessive reactive oxygen species (ROS). Oxidative damage from free radicals, generated by cellular components of semen, is a critical contributor to reduced sperm fertility (Banday, Lone, Rasool, Rashid, & Shikari, ; Bucak et al, ). However, in the present study, adding 2 mM GA to semen extender resulted in frozen‐thawed spermatozoa with significantly highest total motility and also highest progressive motility (albeit not significantly different from controls).…”

Section: Discussionmentioning

confidence: 99%

Gallic and carnosic acids improve quality of frozen‐thawed ram spermatozoa

et al. 2019

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The objective was to determine effects of gallic acid (GA) and carnosic acid (CA), present in carob pods and rosemary extract respectively, on frozen-thawed ram spermatozoa. Thirty ejaculates were collected from five Merino rams, pooled, diluted in Tris-based extender and divided into five equal portions containing: 0.05 or 2 mM of GA; 0.05 or 0.2 mM of CA; or no additive (control). Extended semen was equilibrated at +4°C, loaded into straws, held 5 cm above liquid nitrogen for 12 min then plunged.Computer-aided sperm analysis was used to assess motility, whereas flow cytometry

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“…Reports have shown that ram semen enrichment with butylated hydroxytoulene, taurine, catalase, quercetin, palmitoleic acid, and kinetin enhances motility or viability compared to the control when stored as liquid at refrigerator or after freeze‐thawing process (Banday et al, ; Câmara et al, ; Eslami et al, ; Rather et al, ; Zadeh Hashem & Eslmi, ). The results of the present experiment indicated that Id at 1, 2 and 4 µM enhanced total motility, FPM, and VCL at 48 and 72 hr of storage.…”

Section: Discussionmentioning

confidence: 99%

“…Reports have shown that ram semen enrichment with butylated hydroxytoulene, taurine, catalase, quercetin, palmitoleic acid, and kinetin enhances motility or viability compared to the control when stored as liquid at refrigerator or after freeze-thawing process (Banday et al, 2017;Câmara et al, 2011;Eslami et al, 2017;Rather et al, 2016;Zadeh Hashem & Eslmi, 2018 experiment. Previous study revealed a correlation between capacities of spermatozoa in fertilization with spermatozoa motility assessed by CASA (Larsen et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…During liquid storage of semen, LPO occurs and increases in a time dependency manner (Eslami, Ghasemiyan, & Zadeh, ; Zadeh Hashem, Haddad, & Eslami, ). Studies showed that substances that have anti‐oxidative properties may be useful to prevent or decrease the detrimental effects of LPO during semen preservation technique (Banday, Lone, Rasool, Rashid, & Shikari, ; Bucak et al, ; Rateb, ; Rather, Islam, Malik, & Lone, ; Sarıözkan, Bucak, Tuncer, Ulutas, & Bilgen, ). Thought enhancing ram semen quality during liquid and cold storage may have some challenges.…”

Section: Introductionmentioning

confidence: 99%

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Influence of idebenone on ram semen quality stored at 4°C

Eslami

Jahan-Roshan

Farrokhi‐Ardabili

2018

Reprod Domestic Animals

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Contents The current study was designed to investigate the effect of idebenone (Id), an antioxidant on ram semen quality. Semen samples were collected, pooled and diluted in a Tris‐based extender supplemented with 0, 1, 2, 4 and 8 µM idebenone. Computer‐assisted sperm analysis was used to evaluate spermatozoa kinematics. Sperm viability and membrane functionality were assessed respectively, by eosin‐nigrosin staining and HOS test. Biochemical assays were carried out to measure different metabolites in spermatozoa and medium at 0, 24, 48 and 72 hr. Total and forward progressive motility were greater in 1, 2 and 4 µM idebenone treated groups compared to control at 24, 48 and 72 hr time points (p < 0.05). Semen supplementation with Id significantly increased viability and functionality of spermatozoa membrane during storage (p < 0.05). Lower amounts of lipid hydroperoxides in medium and spermatozoa were observed in Id‐treated groups compared to control one at 24 and 48 hr of storage (p < 0.05). Medium and spermatozoa amounts of malondialdehyde and nitric oxide were less in Id 4 µM group compared to the control at 72 hr (p < 0.05). Total antioxidant capacity values and superoxide dismutase activity of spermatozoa and medium were greater in 2 and 4 µM idebenone treated groups in comparison with the control at 72 hr (p < 0.05). Results of the current study indicated that ram semen supplementation with Id at 4 µM level improved quality by ameliorating nitrosative and peroxidative stress, hence could be considered as an antioxidant additive during storage at 4°C.

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Use of antioxidants reduce lipid peroxidation and improve quality of crossbred ram sperm during its cryopreservation

Cited by 85 publications

References 52 publications

Canthaxanthin protects human sperm parameters during cryopreservation

Canthaxanthin protects human sperm parameters during cryopreservation

Gallic and carnosic acids improve quality of frozen‐thawed ram spermatozoa

Influence of idebenone on ram semen quality stored at 4°C

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