Influence of idebenone on ram semen quality stored at 4°C

Abstract

Contents The current study was designed to investigate the effect of idebenone (Id), an antioxidant on ram semen quality. Semen samples were collected, pooled and diluted in a Tris‐based extender supplemented with 0, 1, 2, 4 and 8 µM idebenone. Computer‐assisted sperm analysis was used to evaluate spermatozoa kinematics. Sperm viability and membrane functionality were assessed respectively, by eosin‐nigrosin staining and HOS test. Biochemical assays were carried out to measure different metabolites in spermatozoa and medium at 0, 24, 48 and 72 hr. Total and forward progressive motility were greater in 1, 2 and 4 µM idebenone treated groups compared to control at 24, 48 and 72 hr time points (p < 0.05). Semen supplementation with Id significantly increased viability and functionality of spermatozoa membrane during storage (p < 0.05). Lower amounts of lipid hydroperoxides in medium and spermatozoa were observed in Id‐treated groups compared to control one at 24 and 48 hr of storage (p < 0.05). Medium and spermatozoa amounts of malondialdehyde and nitric oxide were less in Id 4 µM group compared to the control at 72 hr (p < 0.05). Total antioxidant capacity values and superoxide dismutase activity of spermatozoa and medium were greater in 2 and 4 µM idebenone treated groups in comparison with the control at 72 hr (p < 0.05). Results of the current study indicated that ram semen supplementation with Id at 4 µM level improved quality by ameliorating nitrosative and peroxidative stress, hence could be considered as an antioxidant additive during storage at 4°C.

Attenuative Role of Idebenone on Deltamethrin Mediated Peroxidative Toxicity of Ram Semen Stored at 4°C

“…Scavenging potential of IDB against toxicity of different reactive oxygen species was shown previously (Mordente et al, 1998). Studies which conducted on ram revealed that IDB treated groups (at proper concentration) had lesser hydroperoxide and malondialdehyde values compared to control sample upon liquid storage or after freezing/thawing process of semen (Eslami et al, 2019;Lone et al, 2019). Probable protective role of IDB may due to attenuation of oxidative and peroxidative toxicity induced by DEL during low temperature liquid semen storage.…”

“…It was reported that cooling of spermatozoa induces irreversible damages, and change the spermatozoa function and fertilization potential (Evans and Maxwell, 1987). Then, addition of protective substances to semen extender have been examined by different studies, to ameliorate oxidative toxicity upon preservation (Câmara et al, 2011;Akalin et al, 2016;Eslami et al, 2019). Current study indicated greater amounts of MDA in DEL exposed group compared to combination groups.…”

“…It was reported that IDB has about 7 times greater antioxidant potential compared to vitamin E (Zhai et al, 2008). Moreover, antioxidative role of IDB was confirmed by in vitro and in vivo studies (Rustin et al, 1999;Abdel Baky et al, 2010;Eslami et al, 2019;Lone et al, 2019). It was reported that pre-treatment of retinal epithelial cells with IDB, reduced the formation of ROS and sustained viability of cells when oxidative toxicity induced by hydrogen peroxide (Arend et al, 2015).…”

“…It was documented that IDB reacts with different reactive oxygen and nitrogen species and reduced the harmful effects of them (Cardoso et al, 1998;Mordente et al, 1998;Erb et al, 2012). In addition, protective role of IDB against oxidative stress during liquid-chilled and cryopreserved ram spermatozoa were reported (Eslami et al, 2019;Lone et al, 2019).…”

Liquid Storage of Ram Semen: Associated Damages and Improvement

“…A synthetic analogue of coenzyme Q10, idebenone (2-(10-hydroxydecyl)-5,6dimethoxy-3-methyl-cyclohexa-2,5-diene-1,4-dione) has been reported as a potent antioxidant [113]. Idebenone has been shown to be an efficient antioxidant additive during 72 h storage at 4˚C, improving cell membrane functionality and viability by ameliorating nitrosative and peroxidative stress [114].…”