Use of antigen‑primed dendritic cells for inducing antitumor immune responses inï¿½vitro in patients with non‑small cell lung cancer

Obleukhova, I. A.; Kiryishina, Nataliya; Falaleeva, S.A.; Lopatnikova, Julia; Kurilin, V. V.; Kozlov, Vadim; Vitsin, Aleksander; Cherkasov, A. P.; Kulikova, E. V.; Sennikov, Sergey

doi:10.3892/ol.2017.7403

Cited by 7 publications

(9 citation statements)

References 47 publications

(44 reference statements)

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“…Many studies have demonstrated that most malignant tumors, including lung cancers, can produce a variety of factors that suppress anti-tumor immunity. Moreover, they have also shown that the maturation rate of DCs in patients with lung cancer is independently associated with survival [97, 98]. The ratio of Tregs and cancer recurrence decreases after DC vaccination combined with cytokine-induced killer (DC/CIK) immunotherapy in patients with NSCLC, indicating that this strategy is generally successful [99].…”

Section: Progress In the Clinical Application Of DC Function Restorationmentioning

confidence: 99%

Impaired dendritic cell functions in lung cancer: a review of recent advances and future perspectives

Wang

Huang

2019

Cancer Communications

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Lung cancer is the leading cause of cancer mortality worldwide. Dendritic cells (DCs) are the key factors providing protective immunity against lung tumors and clinical trials have proven that DC function is reduced in lung cancer patients. It is evident that the immunoregulatory network may play a key role in the failure of the immune response to terminate tumors. Lung tumors likely employ numerous strategies to suppress DC-based anti-tumor immunity. Here, we summarize the recent advances in our understanding on lung tumor-induced immunosuppression in DCs, which affects the initiation and development of T-cell responses. We also describe which existing measures to restore DC function may be useful for clinical treatment of lung tumors. Furthering our knowledge of how lung cancer cells alter DC function to generate a tumor-supportive environment will be essential in order to guide the design of new immunotherapy strategies for clinical use.

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Section: Progress In the Clinical Application Of DC Function Restorationmentioning

confidence: 99%

Impaired dendritic cell functions in lung cancer: a review of recent advances and future perspectives

Wang

Huang

2019

Cancer Communications

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“…Furthermore, we found that the level of aDCs and response to Type II IFN in the low-risk group was higher than in the high-risk group, suggesting that it was related to the better prognosis of LUAD patients. DCs are important antigen presenting cells, and reduction of DCs in peripheral blood of patients with non-small cell lung cancer is related to tumor development ( 46 ). One study showed that CD73-ADC treatment can induce a strong intracellular accumulation of pro-inflammatory macrophages and activated dendritic cells in lung cancer cells, which is effective in inhibiting CD73-dysregulated tumors and improving immune responses ( 47 ).…”

Section: Discussionmentioning

confidence: 99%

Nitrogen Metabolism Disorder Accelerates Occurrence and Development of Lung Adenocarcinoma: A Bioinformatic Analysis and In Vitro Experiments

et al. 2022

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BackgroundNitrogen metabolism (NM) plays a pivotal role in immune regulation and the occurrence and development of cancers. The aim of this study was to construct a prognostic model and nomogram using NM-related genes for the evaluation of patients with lung adenocarcinoma (LUAD).MethodsThe differentially expressed genes (DEGs) related to NM were acquired from The Cancer Genome Atlas (TCGA) database. Consistent clustering analysis was used to divide them into different modules, and differentially expressed genes and survival analysis were performed. The survival information of patients was combined with the expressing levels of NM-related genes that extracted from TCGA and Gene Expression Omnibus (GEO) databases. Subsequently, univariate Cox analysis and the least absolute shrinkage and selection operator (LASSO) regression were used to build a prognostic model. GO and KEGG analysis were elaborated in relation with the mechanisms of NM disorder (NMD). Meanwhile, immune cells and immune functions related to NMD were discussed. A nomogram was built according to the univariate and multivariate Cox analysis to identify independent risk factors. Finally, real-time fluorescent quantitative PCR (RT-PCR) and Western bolt (WB) were used to verify the expression level of hub genes.ResultsThere were 138 differential NM-related genes that were divided into two gene modules. Sixteen NM-related genes were used to build a prognostic model and the receiver operating characteristic curve (ROC) showed that the efficiency was reliable. GO and KEGG analysis suggested that NMD accelerated development of LUAD through the Wnt signaling pathway. The level of activated dendritic cells (aDCs) and type II interferon response in the low-risk group was higher than that of the high-risk group. A nomogram was constructed based on ABCC2, HMGA2, and TN stages, which was identified as four independent risk factors. Finally, RT-PCR and WB showed that CDH17, IGF2BP1, IGFBP1, ABCC2, and HMGA2 were differently expressed between human lung fibroblast (HLF) cells and cancer cells.ConclusionsHigh NM levels were revealed as a poor prognosis of LUAD. NMD regulates immune system through affecting aDCs and type II interferon response. The prognostic model with NM-related genes could be used to effectively evaluate the outcomes of patients.

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“…IFNγ receptor impairment or IFN-γ-mediated signal disruption may cause insensitivity to IFN-γ, which may promote the development and progress of tumors [75]. Dendritic cells (DCs) were associated with the regulation of the immune response and played key roles in inducing anti-tumor activity [76], a decrease in immature dendritic cells (iDC) and impaired migration ability may cause cancer [77].…”

Section: Discussionmentioning

confidence: 99%

Prognostic Ability of Enhancer RNAs in Metastasis of Non-Small Cell Lung Cancer

Liu

Wang²,

Zhang³

et al. 2022

Molecules

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(1) Background: Non-small cell lung cancer (NSCLC) is the most common lung cancer. Enhancer RNA (eRNA) has potential utility in the diagnosis, prognosis and treatment of cancer, but the role of eRNAs in NSCLC metastasis is not clear; (2) Methods: Differentially expressed transcription factors (DETFs), enhancer RNAs (DEEs), and target genes (DETGs) between primary NSCLC and metastatic NSCLC were identified. Prognostic DEEs (PDEEs) were screened by Cox regression analyses and a predicting model for metastatic NSCLC was constructed. We identified DEE interactions with DETFs, DETGs, reverse phase protein arrays (RPPA) protein chips, immunocytes, and pathways to construct a regulation network using Pearson correlation. Finally, the mechanisms and clinical significance were explained using multi-dimensional validation unambiguously; (3) Results: A total of 255 DEEs were identified, and 24 PDEEs were selected into the multivariate Cox regression model (AUC = 0.699). Additionally, the NSCLC metastasis-specific regulation network was constructed, and six key PDEEs were defined (ANXA8L1, CASTOR2, CYP4B1, GTF2H2C, PSMF1 and TNS4); (4) Conclusions: This study focused on the exploration of the prognostic value of eRNAs in the metastasis of NSCLC. Finally, six eRNAs were identified as potential markers for the prediction of metastasis of NSCLC.

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Use of antigen‑primed dendritic cells for inducing antitumor immune responses inï¿½vitro in patients with non‑small cell lung cancer

Cited by 7 publications

References 47 publications

Impaired dendritic cell functions in lung cancer: a review of recent advances and future perspectives

Impaired dendritic cell functions in lung cancer: a review of recent advances and future perspectives

Nitrogen Metabolism Disorder Accelerates Occurrence and Development of Lung Adenocarcinoma: A Bioinformatic Analysis and In Vitro Experiments

Prognostic Ability of Enhancer RNAs in Metastasis of Non-Small Cell Lung Cancer

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