Use of Anterior Segment Optical Coherence Tomography to Predict Corneal Graft Rejection in Small Animal Models

Liu, Yu-Chi; Lwin, Nyein Chan; Chan, Nicole Shu Wen; Mehta, Jodhbir S.

doi:10.1167/iovs.14-14475

Cited by 29 publications

(29 citation statements)

References 14 publications

(22 reference statements)

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“…Using this technology, we have demonstrated that central corneal thickening is strongly associated with graft rejection in mice. Our data align well with a recent rat study by Liu et al 19 In the current study, we have also shown that central corneal thickening can be interfered with a molecular intervention, such as anti-Ang-2 treatment. These results together support OCT as a new and reliable technology to monitor murine corneal grafts in vivo.…”

Section: Discussionsupporting

confidence: 93%

Angiopoietin-2 Blockade Promotes Survival of Corneal Transplants

Zhang

Sessa

et al. 2017

Invest. Ophthalmol. Vis. Sci.

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PurposeCorneal transplantation remains the last hope for vision restoration, and lymphangiogenesis (LG) is a primary mediator of transplant rejection. This study was to investigate the specific role of angiopoietin-2 (Ang-2) in transplantation-associated LG and graft rejection.MethodsOrthotopic corneal transplantation was performed between fully mismatched C57BL/6 (donor) and BALB/c (recipient) mice to assess the effects of Ang-2 blockade via neutralizing antibody. Grafts were evaluated in vivo by ophthalmic slit-lamp biomicroscopy and anterior segment optical coherence tomography (OCT) up to 8 weeks after surgery. Additionally, whole-mount corneas were analyzed for lymphatic and blood vessels and macrophages by immunofluorescent microscopy, and draining lymph nodes were assessed for donor-derived cells by flow cytometry.ResultsAnti-Ang-2 treatment significantly suppressed LG and graft rejection. In this study, we achieved 75% suppression of LG and 80% graft survival. Our approach also inhibited donor-derived cell trafficking to draining lymph nodes and affected macrophage morphologic phenotypes in the grafted corneas. Additionally, Ang-2 blockade also reduced central corneal thickening, a parameter strongly associated with graft rejection.ConclusionsAng-2 is critically involved in corneal transplant rejection and anti-Ang-2 treatment significantly improves the outcomes of corneal grafts. Moreover, we have shown that anterior segment OCT offers a new tool to monitor murine corneal grafts in vivo. This study not only reveals new mechanisms for transplant rejection, but also offers a novel strategy to treat it.

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Section: Discussionsupporting

confidence: 93%

Angiopoietin-2 Blockade Promotes Survival of Corneal Transplants

Zhang

Sessa

et al. 2017

Invest. Ophthalmol. Vis. Sci.

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“…Therefore, the sutures were not removed in some studies; in contrast, graft sutures were removed in other studies to mimic penetrating keratoplasty in patients. 16,42,43 However, the suture removal time varied from 4 days to 2 weeks after operation and was not consistent among these studies. The optimal suture removal time should be determined in future research.…”

Section: Discussionmentioning

confidence: 89%

“…Graft transparency, edema, and neovascularization parameters were used to evaluate the corneal grafts according to the previously established scoring system. [15][16][17] Three parameters have five grades (0-4 scores) respectively (Table). When the total scores of these three parameters are equal or greater than six scores, the graft is identified as rejection.…”

Section: Corneal Transplantation and Definition Of Graft Rejectionmentioning

confidence: 99%

Hemin Promotes Corneal Allograft Survival Through the Suppression of Macrophage Recruitment and Activation

Dai

Cheng

et al. 2018

Invest. Ophthalmol. Vis. Sci.

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“…Similar to its clinical application, SD‐OCT has facilitated the monitoring of graft outcomes, with longitudinal measurements of corneal oedema and quantification of inflammation, up to 28 days post‐operatively, in rodent models . This application of AS‐OCT also demonstrates the potential to use SD‐OCT as a grading tool for corneal graft rejection.…”

Section: As‐oct Imaging In Animalsmentioning

confidence: 93%

“…Similar to its clinical use, novel applications of AS‐OCT to longitudinally monitor and quantitate the extent of inflammation have been documented in multiple experimental models of ocular inflammation, including uveitis, microbial keratitis, sterile inflammation and dry eye disease . Additionally, AS‐OCT has been used to monitor the resolution of inflammation in experimental models of corneal transplantation and corneal wound healing . In these contexts, AS‐OCT has the unique capacity to obtain in vivo information regarding corneal inflammation and the response to treatment.…”

Section: As‐oct Imaging In Animalsmentioning

confidence: 99%

Anterior segment optical coherence tomography: its application in clinical practice and experimental models of disease

Jiao

Hill

Downie

et al. 2019

Clinical and Experimental Optometry

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Optical coherence tomography (OCT) provides non-invasive, high-resolution in vivo imaging of the ocular surface and anterior segment. Over the years, it has become an essential tool for evaluating the anterior segment of the eye to monitor ocular development and ocular pathologies in both the clinical and research fields of ophthalmology and optometry. In this review, the clinical applications relating to the use of anterior segment OCT for imaging and quantifying normal and pathological features of the ocular surface, cornea, anterior chamber, and aqueous outflow system are summarised in a range of human ocular diseases. Applications of anterior segment OCT technology that have improved imaging and quantitation of ocular inflammation in experimental animal models of ocular diseases, such as anterior uveitis, microbial keratitis and glaucoma, are also described. The capacity to longitudinally evaluate anterior segment anatomical changes during development, and inflammation facilitates the understanding of the dynamics of tissue responses, and further enhances the intra-operative in vivo imaging during procedures, such as corneal transplantation and drug delivery. Future developments including in vivo ultrahigh-resolution anterior segment OCT, automated analyses of anterior segment OCT images and functional extensions of the technique, may revolutionise the clinical evaluation of anterior segment, corneal and ocular surface diseases.

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Use of Anterior Segment Optical Coherence Tomography to Predict Corneal Graft Rejection in Small Animal Models

Abstract: The use of ASOCT to evaluate AC inflammation and corneal thickness aids in the early evaluation and diagnosis of graft rejection in animal models. Early increased AC inflammation was an early predictor of graft failure prior to definitive clinical evaluation.

Cited by 29 publications

References 14 publications

Angiopoietin-2 Blockade Promotes Survival of Corneal Transplants

Angiopoietin-2 Blockade Promotes Survival of Corneal Transplants

Hemin Promotes Corneal Allograft Survival Through the Suppression of Macrophage Recruitment and Activation

Anterior segment optical coherence tomography: its application in clinical practice and experimental models of disease

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