Use of an online extraction liquid chromatography quadrupole time-of-flight tandem mass spectrometry method for the characterization of polyphenols in Citrus paradisi cv. Changshanhuyu peel

Tong, Chaoying; Peng, Mijun; Tong, Runna; Ma, Rujiang; Guo, Keke; Shi, Shuyun

doi:10.1016/j.chroma.2017.12.022

Cited by 22 publications

(11 citation statements)

References 35 publications

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“…Acid pH between 2 and 4, contribute to avoiding phenolic compounds dissociation, help with defining peaks and improving the ionization efficiency for mass characterization [71,78]. Tong et al [79] optimized different concentrations of acetic or formic acid in two mobile phases (water-methanol or water-acetonitrile) and also various gradient programs to determine several polyphenols in Citrus paradisi cv. Changshanhuyu peel.…”

Section: Chromatographic and Mass Spectrometry Conditionsmentioning

confidence: 99%

“…Despite the differences and advantages reported for tandem mass spectrometers, multiple types of mass spectrometers can be used for polyphenol analysis, such as quadrupole (single or triple) [49,50], ion trap mass spectrometer [80][81][82], time-of flight or quadrupole-time-of-flight [10,79,80,83], and Orbitrap [33] among others. In the consulted literature there are studies demonstrating efficacy in polyphenols detection and quantification with different mass analyzers (Table 3).…”

Section: Chromatographic and Mass Spectrometry Conditionsmentioning

confidence: 99%

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Determination of Polyphenols Using Liquid Chromatography–Tandem Mass Spectrometry Technique (LC–MS/MS): A Review

et al. 2020

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In recent years, the consumption of polyphenols has been increasing, largely due to its beneficial effects on health. They are present in a wide variety of foods, but their extraction and characterization are complicated since they are mostly in complex matrices. For this reason, the use of selective, sensitive, and versatile analytical techniques such as liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) is necessary. In this review, the most relevant studies of the last years regarding the analysis of polyphenols in different matrices by comprehensive LC–MS/MS are discussed. Relevant steps such as extraction, sample purification, and chromatographic analysis methods are emphasized. In particular, the following methodological aspects are discussed: (a) the proper selection of the extraction technique, (b) the extraction and elution solvents, (c) the purification step, (d) the selection of both stationary and mobile phases for the chromatographic separation of compounds, and (e) the different conditions for mass spectrometry. Overall, this review presents the data from the most recent studies, in a comprehensive way, thus providing and simplifying the information of the great variety of works that exist in the literature on this wide topic.

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Section: Chromatographic and Mass Spectrometry Conditionsmentioning

confidence: 99%

Section: Chromatographic and Mass Spectrometry Conditionsmentioning

confidence: 99%

Determination of Polyphenols Using Liquid Chromatography–Tandem Mass Spectrometry Technique (LC–MS/MS): A Review

et al. 2020

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“…7.0 mg of P1 at 98.3% purity, 20.8 mg of P2 at 97.3% purity). The high extraction efficiency for OLE‒HSCCC system could be ascribed to the presence of better pressure for solvent inside the matrix .…”

Section: Resultsmentioning

confidence: 99%

Direct from solid natural products to pure compounds in a single step: Coupling online extraction with high‐speed counter‐current chromatography

Guo

Peng

Tong

et al. 2018

J of Separation Science

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Extraction is the most important step in the purification of bioactive compounds from natural products. This study introduces a simple online extraction strategy coupled with high-speed counter-current chromatography for efficient extraction and purification of bioactive components from solid natural products. For online extraction strategy, 1.0 g of ground Mangnolia officinalis or Piper nigrum was loaded into a guard column, which was then positioned on the manual injection valve instead of the sample loop. Bioactive components were directly extracted by the mobile phase of high-speed counter-current chromatography, and then transferred into high-speed counter-current chromatography for purification. In addition, the compatibility of the developed methodology for direct purification of bioactive components from fresh M. officinalis was successfully demonstrated. Obviously, in comparison with traditional offline heat-reflux extraction, online extraction avoided the instrument, time, solvent, and energy consumption, and purified two phenolic compounds (honokiol and magnolol) from M. officinalis and three alkaloids (piperyline, piperine, and piperanine) from P. nigrum with high extraction efficiency. The superiority of the developed methodology is to establish an easy, rapid, and efficient technique for the purification of a wide variety of bioactive components from solid natural products.

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“…Compound 33 showed the same molecular ions and fragment ions with those of 6-hydroxyapigenin-7-O-glucoside (27); therefore, it could be identified as 6-hydroxyapigenin-4′-O-glucoside [33]. 7, p-hydroxybenzoic acid (9), caffeic acid (11), syringic acid (12), and ferulic acid (21) have been found in the other species of Plantago genus (e.g., P. major, P. asiatica) [12], and cis-3-O-caffeoylquinic acid (6) was reported in Plantago family for the first time. [29].…”

Section: Identification Of Phenylethanoid Glycosidesmentioning

confidence: 98%

Comprehensive Polyphenolic Profile of Plantago depressa using High‐Speed Countercurrent Chromatography Off‐line with High‐Performance Liquid Chromatography–Diode Array Detector–Quadrupole Time‐of‐flight Tandem Mass Spectrometry

Xu¹,

Tong²,

Fu³

et al. 2019

eFood

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The whole plant of Plantago depressa is commonly used as traditional Chinese medicine and functional food ingredient. Total phenolic content and total flavonoid content evaluation indicated that its ethyl acetate and n-butanol fractions were rich in polyphenols. However, to date, the polyphenolic profile is limited because of the complex matrix. Here, polyphenols in ethyl acetate and n-butanol fractions of P. depressa were first separated and enriched by High-Speed Countercurrent Chromatography (HSCCC). Then, their structures were characterized or tentatively identified by High-Performance Liquid Chromatography (HPLC)-Diode Array Detector (DAD)-Quadrupole Time-of-Flight Tandem Mass Spectrometry (QTOF-MS/MS) (HSCCC × HPLC-DAD-QTOF-MS/MS). Because of the orthogonality of HSCCC and HPLC, 37 polyphenols were identified. Notably, 29 polyphenols were discovered from P. depressa for the first time. Finally, contents of two potential new compounds, 2-ethoxy plantamajoside isomers (17 and 29), were determined as 0.27 ± 0.03 and 0.04 ± 0.01 mg/g, respectively. Results indicated that P. depressa could be used as a promising source of polyphenols for health promotion, and the developed method (HSCCC × HPLC) was efficient to explore polyphenolic profile for complex natural products.

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Use of an online extraction liquid chromatography quadrupole time-of-flight tandem mass spectrometry method for the characterization of polyphenols in Citrus paradisi cv. Changshanhuyu peel

Cited by 22 publications

References 35 publications

Determination of Polyphenols Using Liquid Chromatography–Tandem Mass Spectrometry Technique (LC–MS/MS): A Review

Determination of Polyphenols Using Liquid Chromatography–Tandem Mass Spectrometry Technique (LC–MS/MS): A Review

Direct from solid natural products to pure compounds in a single step: Coupling online extraction with high‐speed counter‐current chromatography

Comprehensive Polyphenolic Profile of Plantago depressa using High‐Speed Countercurrent Chromatography Off‐line with High‐Performance Liquid Chromatography–Diode Array Detector–Quadrupole Time‐of‐flight Tandem Mass Spectrometry

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