Use of an electro-optical sensor and phage antibodies for immunodetection of Herbaspirillum

Guliy, О. I.; Velichko, Natalya S.; Fedonenko, Yu. P.; Bunin, Victor D.

doi:10.1016/j.talanta.2019.04.086

Cited by 12 publications

(3 citation statements)

References 42 publications

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“…The same conclusion was obtained from the host spectrum test that phages can specifically identify Salmonella host bacteria. The biosensor constructed exhibits more excellence than immunodetection using an electro-optical sensor [ 71 ]. The results also validated the rationality of the sensor preparation process.…”

Section: Resultsmentioning

confidence: 99%

Properties of a Novel Salmonella Phage L66 and Its Application Based on Electrochemical Sensor-Combined AuNPs to Detect Salmonella

Wang

et al. 2022

Foods

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Salmonella is widespread in nature and poses a significant threat to human health and safety. Phage is considered as a new tool for the control of food-borne pathogens. In this study, Salmonella phage L66 (phage L66) was isolated from sewage by using Salmonella Typhimurium ATCC 14028 as the host bacterium, and its basic properties were obtained by biological and bioinformatics analysis. Phage L66 had a broad host spectrum, with an optimal infection complex of 0.1 and an optimal adsorption rate of 90.06%. It also exhibited thermal stability between 30 °C~60 °C and pH stability pH from 3 to 12, and the average lysis amount was 46 PFU/cell. The genome sequence analysis showed that the genome length of phage L66 was 157,675 bp and the average GC content was 46.13%. It was predicted to contain 209 genes, 97 of which were annotated with known functions based on the evolutionary analysis, and phage L66 was attributed to the Kuttervirus genus. Subsequently, an electrochemical sensor using phage L66 as a recognition factor was developed and the working electrode GDE-AuNPs-MPA-Phage L66 was prepared by layer-by-layer assembly for the detection of Salmonella. The slope of the impedance was 0.9985 within the scope from 20 to 2 × 107 CFU/mL of bacterial concentration. The minimum detection limit of the method was 13 CFU/mL, and the average spiked recovery rate was 102.3% with a relative standard deviation of 5.16%. The specificity and stability of this sensor were excellent, and it can be applied for the rapid detection of Salmonella in various foods. It provides a phage-based electrochemical biosensor for the detection of pathogenic bacteria.

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Section: Resultsmentioning

confidence: 99%

Properties of a Novel Salmonella Phage L66 and Its Application Based on Electrochemical Sensor-Combined AuNPs to Detect Salmonella

Wang

et al. 2022

Foods

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“…Measurements were made with an ELUS EO analyzer (EloSystems GbR, Germany), as described in [ 28 ]. The measuring conditions were: electric field strength, 89.4 V/cm; light wavelength, 670 nm (relative to vacuum); field application time, 4.5 s. Before study, the bacteria were washed by double centrifugation at 2800 g for 5 min in distilled water (conductivity, 1.8 μS/cm).…”

Section: Methodsmentioning

confidence: 99%

Use of Azospirillum baldaniorum cells in quercetin detection

et al. 2023

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The possibility of detection and determination of flavonoids by using microbial cells was shown for the first time using the quercetin - Azospirillum baldaniorum Sp245 model system. The activity of the flavonoids quercetin, rutin and naringenin toward A. baldaniorum Sp245 was evaluated. It was found that when the quercetin concentration ranged from 50 to 100 µM, the number of bacterial cells decreased. Rutin and naringenin did not affect bacterial numbers. Quercetin at 100 μM increased bacterial impedance by 60 %. Under the effect of quercetin, the magnitude of the electro-optical signal from cells decreased by 75 %, as compared with the no-quercetin control. Our data show the possibility of developing sensor-based systems for the detection and determination of flavonoids.

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“…The low-frequency portion of the frequency dispersion of the electro-optical signal is associated with changes in the surface properties of cells (Figure 18). It is used to detect changes in cells after exposure to monoclonal, polyclonal, and phage antibodies in the range of cell densities being detected 10 8 -10 4 cells mL −1 [167][168][169]. The frequency dispersion and absolute value of the signal before any effect serves as a standard for evaluating changes in the state of the cell surface.…”

Section: Electro-optical Sensors With a Fixed Direction Of The Electr...mentioning

confidence: 99%

Optical Sensors for Bacterial Detection

Guliy,

Karavaeva,

Smirnov

et al. 2023

Sensors

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Analytical devices for bacterial detection are an integral part of modern laboratory medicine, as they permit the early diagnosis of diseases and their timely treatment. Therefore, special attention is directed to the development of and improvements in monitoring and diagnostic methods, including biosensor-based ones. A promising direction in the development of bacterial detection methods is optical sensor systems based on colorimetric and fluorescence techniques, the surface plasmon resonance, and the measurement of orientational effects. This review shows the detecting capabilities of these systems and the promise of electro-optical analysis for bacterial detection. It also discusses the advantages and disadvantages of optical sensor systems and the prospects for their further improvement.

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Use of an electro-optical sensor and phage antibodies for immunodetection of Herbaspirillum

Cited by 12 publications

References 42 publications

Properties of a Novel Salmonella Phage L66 and Its Application Based on Electrochemical Sensor-Combined AuNPs to Detect Salmonella

Properties of a Novel Salmonella Phage L66 and Its Application Based on Electrochemical Sensor-Combined AuNPs to Detect Salmonella

Use of Azospirillum baldaniorum cells in quercetin detection

Optical Sensors for Bacterial Detection

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