Use of an Andersen Bioaerosol Sampler to Simultaneously Provide Culturable Particle and Culturable Organism Size Distributions

King, Maria D.; McFarland, Andrew R.

doi:10.1080/02786826.2012.669507

Cited by 14 publications

(12 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In some cases, particles were collected in stages designed to capture larger sizes. Similar results were also seen by King and McFarland (2012), where 1.8 mm particles deposited on the 2.1 to 3.3 mm stage. This may be due to the particles not completely drying and therefore having a larger aerodynamic size compared to dry PSL particles.…”

Section: Discussionsupporting

confidence: 85%

“…Several studies have modified cascade impactor collection methods to acquire more information than what is provided by traditional use of agar-based impactors. Recent research found that each airborne biological particle can be comprised of many organisms; for example, a 3 mm bio-cluster particle may contain up to 32 organisms (Kesavan et al 2014;King and McFarland 2012). King and McFarland (2012) used a modified six-stage bio-cascade impactor method to determine both the aerosol size distribution and the number of organisms.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sampling efficiencies of two modified viable cascade impactors

Kesavan

Rule

2017

Aerosol Science and Technology

View full text Add to dashboard Cite

Prevention of airborne contagious diseases depends on successful characterization of aerosols in the environment. The use of cascade impactors to characterize ambient aerosols is one of the most commonly used methods, providing data on both particle size and concentration. In this study, the use of a cascade impactor recently described in the literature using 8 mL of liquid in Petri dishes (CI-L) was compared with a new method that uses wet membrane filters on top of wax filled Petri dishes (CI-WWMF). Sampling efficiencies of the cascade impactors were evaluated using 0.5, 1, 3, and 5 mm polystyrene latex (PSL) microspheres and aerosol consisting of single spores of Bacillus atrophaeus var. globigii (BG). The sampling efficiency of the CI-L was 6%, 11%, 17%, 21%, and 58% for 0.5, 1, 3, 5 mm PSL microspheres and BG spores, respectively. Higher overall sampling efficiencies of 71%, 91%, 60%, 64%, and 104% were observed for the same size and type of particles for the CI-WWMF. This study indicates that using wet filters on top of wax-filled Petri dishes (CI-WWMF) in a viable cascade impactor is more efficient than the CI-L method for size-selectively collecting biological aerosols from the environment. The CI-WWMF method is useful when a liquid medium is required for identifying and quantifying organisms using polymerase chain reaction (PCR) and immuno-assay techniques.

show abstract

Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Sampling efficiencies of two modified viable cascade impactors

Kesavan

Rule

2017

Aerosol Science and Technology

View full text Add to dashboard Cite

show abstract

“…Our results are in accordance with previous reports made for different matrices [ 45 ]. The atmospheric bacterial concentrations obtained in our case study, using the flow cytometry, are in accordance with observations performed in other locations [ 65 , 66 ]. However, to date, observations mainly reflect large bacteria since some bacteria counts are still missing due to the sample complexity and the presence of very small bacteria or spores present in the background noise of flow cytometry.…”

Section: Methodssupporting

confidence: 90%

Atmospheric Biodetection Part I: Study of Airborne Bacterial Concentrations from January 2018 to May 2020 at Saclay, France

Sarda‐Estève

Baisnée

Guinot

et al. 2020

IJERPH

View full text Add to dashboard Cite

Background: The monitoring of bioaerosol concentrations in the air is a relevant endeavor due to potential health risks associated with exposure to such particles and in the understanding of their role in climate. In this context, the atmospheric concentrations of bacteria were measured from January 2018 to May 2020 at Saclay, France. The aim of the study was to understand the seasonality, the daily variability, and to identify the geographical origin of airborne bacteria. Methods: 880 samples were collected daily on polycarbonate filters, extracted with purified water, and analyzed using the cultivable method and flow cytometry. A source receptor model was used to identify the origin of bacteria. Results: A tri-modal seasonality was identified with the highest concentrations early in spring and over the summer season with the lowest during the winter season. Extreme changes occurred daily due to rapid changes in meteorological conditions and shifts from clean air masses to polluted ones. Conclusion: Our work points toward bacterial concentrations originating from specific seasonal-geographical ecosystems. During pollution events, bacteria appear to rise from dense urban areas or are transported long distances from their sources. This key finding should drive future actions to better control the dispersion of potential pathogens in the air, like persistent microorganisms originating from contaminated areas.

show abstract

“…With its great recovery efficiency, the Andersen 1‐STG with TSA may be considered as a sampling method in places where low levels of airborne S. aureus are expected (e.g., hospitals) and when the BioSampler is unavailable or unsuitable due to practical consideration (e.g., fragile because of its glass construction). Further quantification of culturable organisms from Andersen samplers is suggested, as more than one culturable cell in a culturable particle likely occur in fields (King and McFarland, ).…”

Section: Resultsmentioning

confidence: 99%

Impact of culture media and sampling methods onStaphylococcus aureusaerosols

Chang

Wang

2014

Indoor Air

View full text Add to dashboard Cite

Staphylococcus aureus has been detected indoors and is associated with human infection. Reliable quantification of S. aureus using a sampling technique followed by culture assay helps in assessing the risks of human exposure. The efficiency of five culture media and eight sampling methods in recovering S. aureus aerosols were evaluated. Methods to extract cells from filters were also studied. Tryptic soy agar (TSA) presented greater bacterial recovery than mannitol salt agar (MSA), CHROMagar staph aureus, Chapman stone medium, and Baird-Park agarose (P < 0.05). Moreover, 93 ± 2%-95 ± 2% and 42 ± 1%-49 ± 2% of S. aureus were, respectively, recovered by a 15-min heating of gelatin filters and 2-min vortex of polycarbonate (PC) filters. Evaluation of two filtration (IOM with gelatin filter and cassette with PC filter), two impaction (Andersen 1-STG loaded with TSA and MSA) and four impingement methods [AGI-30 and BioSampler filled with Tween mixture (TM) and phosphate-buffered saline (PBS)] revealed the BioSampler/TM performed best over 30 and 60 min of sampling (P < 0.05), while low recovery efficiencies were associated with the IOM/gelatin, cassette/PC, and AGI-30/PBS combinations (P < 0.05). In addition to BioSampler/TM, collecting S. aureus onto TSA from the Andersen 1-STG is also recommended, as it is the second best method at the 60-min sampling (P < 0.05).

show abstract

Use of an Andersen Bioaerosol Sampler to Simultaneously Provide Culturable Particle and Culturable Organism Size Distributions

Cited by 14 publications

References 16 publications

Sampling efficiencies of two modified viable cascade impactors

Sampling efficiencies of two modified viable cascade impactors

Atmospheric Biodetection Part I: Study of Airborne Bacterial Concentrations from January 2018 to May 2020 at Saclay, France

Impact of culture media and sampling methods onStaphylococcus aureusaerosols

Contact Info

Product

Resources

About