Use of activated carbon for removing phenylalanine from reconstituted skim milk powder hydrolysates

Lopes, Daniella Cristine Fialho; Delvivo, Fernanda Meneghello; Silvestre, Marialice Pinto Coelho

doi:10.1016/j.lwt.2004.07.021

Cited by 33 publications

(44 citation statements)

References 18 publications

(32 reference statements)

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“…It has been reported that activated carbon could selectively adsorb Trp and Phe amino acids or peptides containing these residues during debittering of milk protein hydrolyzates [13]. Activated carbon has been successfully used for the removal of Phe from food-protein hydrolyzates, notably to develop low-Phe content foods for phenylketonuric patients [55,77]. Other uses of activated carbon have been to selectively prepare dipeptide mixtures with a high Fischer ratio (branched chain amino acids/ aromatic amino acids) with the low Fisher ratio peptides being adsorbed on the activated carbon [2].…”

Section: Discussionmentioning

confidence: 99%

Tryptophan-containing milk protein-derived dipeptides inhibit xanthine oxidase

2012

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Section: Discussionmentioning

confidence: 99%

Tryptophan-containing milk protein-derived dipeptides inhibit xanthine oxidase

2012

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“…A avaliação da eficiência de remoção de Phe pelo carvão ativado foi realizada pela medida do teor de Phe livre, no EPA e seus hidrolisados (após tratamento com carvão), empregando-se a técnica desenvolvida no mesmo laboratório em que o presente estudo foi realizado (LOPES et al, 2004), baseada na Espectrofotometria Derivada Segunda (EDS). Desta forma, as soluções de hidrolisados (0,8 g/100 mL de água purificada), previamente submetidas ao tratamento com carvão ativado, e a solução de EPA (0,2 g/100 g de água purificada), foram colocadas em tubos especiais de hidrólise e evaporadas em Centrivap (Labconco, EUA) até resíduo.…”

Section: Avaliação Da Remoção De Pheunclassified

“…agrotec., Lavras, v. 30, n. 2, p. 308-316, mar./abr., 2006 multidetecção das frações a 230, 280 e 300 nm, para se eliminar a interferência da absorção dos aminoácidos aromáticos, calcula-se a ACF. Esta, por sua vez, é levada a uma curva padrão, para se encontrar a quantidade de peptídeos de cada fração, preparada como descrito em outros trabalhos (LOPES et al, 2004;MORATO et al, 2000;SILVESTRE et al, 1994b).…”

Section: Caracterização Do Perfil Peptídico Dos Hidrolisados Protéicounclassified

“…A EDS tem sido utilizada com êxito em diversos trabalhos. (DELVIVO et al, 2004;LOPES et al, 2004;MARCO, 2004;MORAIS et al, 2005;SILVA, 2004;SILVESTRE et al, 1993;SOARES et al, 2004).…”

Section: Introductionunclassified

“…(CARREIRA et al, 2002;DELVIVO et al, 2004;LOPES et al, 2004;MARCO, 2004;MORATO et al, 2000;MORAIS et al, 2005;SILVA, 2004;SOARES et al, 2004). É interessante que se conheça o perfil peptídico de hidrolisados protéicos para uso em dietas especiais, pois, segundo González-Tello (1994), eles devem apresentar as seguintes características: alto teor de di-e tri-peptídeos, massa molecular média de 500 Da e não conter peptídeos maiores que 1000 Da.…”

Section: Introductionunclassified

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Hidrolisados protéicos de arroz com baixo teor de finilalanina, obtidos pela ação da corolase PP e uso do carvão ativado

et al. 2006

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RESUMOA partir do extrato protéico obtido em meio alcalino, empregou-se a corolase PP no preparo de seis hidrolisados enzimáticos de arroz, visando o desenvolvimento de formulações dietéticas para fenilcetonúricos. O procedimento de passagem por coluna contendo carvão ativado foi utilizado na remoção de fenilalanina (Phe) dos hidrolisados. O teor de Phe nos extratos protéicos de arroz e seus hidrolisados, após tratamento com carvão, foi determinado por espectrofotometria derivada segunda. O teor final de fenilalanina nos hidrolisados variou de 0,39 a 68,34 mg 100 g -1 , o que corresponde a uma remoção de 84 a 100%. A distribuição dos peptídeos nas amostras, de acordo com o tamanho da cadeia, foi estudada como um dos critérios para a avaliação da qualidade nutricional dos hidrolisados. O fracionamento dos peptídeos foi realizado por CLAE de exclusão molecular e, para a quantificação empregou-se o método rápido da área corrigida da fração. Os resultados indicaram que o maior teor de oligopeptídeos foi obtido quando se empregou a corolase PP em uma relação E:S de 1%, e uma concentração protéica inicial de 1,56 g 100 mL -1 . Termos para indexação:Hidrólise enzimática, remoção de fenilalanina, suplemento dietético. ABSTRACTStarting with alkaline protein extract, the Corolase PP was used for preparing six enzymatic rice hydrolysates, with the aim of producing dietary formulations for phenylketonuria treatment. A column containing activated pure charcoal was used for removing phenylalanine (Phe). The Phe contents of rice protein extract and hydrolysates were determined by second derivative spectrophotometry. The final Phe content of the hydrolysates varied from 0.39 to 68.34 mg 100 g -1 , corresponding to removal levels from 84% to 100%. The distribution of peptides according to their size was used as an approach for the nutritional evaluation of the hydrolysates. The fractionation of peptides was performed by size-exclusion HPLC, and the rapid correct fraction area method was used for the their quantification. The results indicated that the higher oligopeptide content was obtained when corolase PP was used with an enzyme:substrate ratio of 1% and the initial protein concentration of 1,56 g 100 mL -1 .

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Adsorption kinetics, isotherms and thermodynamics of aromatic amino acids in feather hydrolysate onto modified activated carbon

Wang,

Ding,

Zhang

et al. 2023

Env Prog and Sustain Energy

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The main objective of this study was to produce low cost activated carbons for the adsorption of aromatic amino acids (AA) from feather hydrolysate. By optimizing the static adsorption conditions (time, pH and concentration) of amino acids, the adsorption amounts of tyrosine and phenylalanine reached 15.2 and 20.1 mg/g, respectively, under optimal conditions. Furthermore, it exhibits excellent reusability as it maintains a removal efficiency of 82.0% and 82.8% for tyrosine and phenylalanine after being reused 4 times. The effects of NaOH modified activated carbon (NA‐AC) on the adsorption capacity of AA in feather hydrolysate at different temperatures (298.15, 308.15, 318.15 K) were investigated, and the static adsorption kinetics curves were plotted. The adsorption isotherm (Langmuir, Freundlich model), kinetics (pseudo‐first‐order‐mode, pseudo‐second‐order‐mode, particle diffusion model) and thermodynamics were studied. The results shown that the adsorption process of AA onto NA‐AC accorded with the pseudo‐second‐order‐mode. The adsorption rate was mainly controlled by the liquid film diffusion and the particle diffusion. The adsorption isotherm accorded with the Langmuir adsorption isothermal model. Adsorption was an endothermic process, and increasing temperature was conducive to adsorption. Gibbs energy ΔG indicated that the adsorption process was spontaneous, and enthalpy △H indicated that the adsorption process was mainly physical adsorption. The proposed adsorption mechanisms provide a theoretical basis for the optimization of AA separation and purification process in feather hydrolysate by NA‐AC, which may further improve the separation and purification efficiency of other amino acids in feather hydrolysate.

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Use of activated carbon for removing phenylalanine from reconstituted skim milk powder hydrolysates

Cited by 33 publications

References 18 publications

Tryptophan-containing milk protein-derived dipeptides inhibit xanthine oxidase

Tryptophan-containing milk protein-derived dipeptides inhibit xanthine oxidase

Hidrolisados protéicos de arroz com baixo teor de finilalanina, obtidos pela ação da corolase PP e uso do carvão ativado

Adsorption kinetics, isotherms and thermodynamics of aromatic amino acids in feather hydrolysate onto modified activated carbon

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