Use of a redox‐sensing GFP (c‐roGFP1) for real‐time monitoring of cytosol redox status in <i>Arabidopsis thaliana</i> water‐stressed plants

Jubany-Marí, Tana; Alegre-Batlle, L.; Jiang, Keni; Feldman, Lewis J.

doi:10.1016/j.febslet.2010.01.014

Cited by 61 publications

(42 citation statements)

References 29 publications

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“…Finally, it is possible to determine the redox potential of the glutathione pool in a high spatial and temporal resolution using various redox-sensitive green fluorescent proteins (for example roGFP) encoded in the test plant. The method requires transient or permanent plant transformation and has already been used in many cases (Beneloujaephajri et al, 2013;Heller et al, 2012;Jubany-Mari et al, 2010;Lehmann et al, 2009;Rosenwasser et al, 2011).…”

Section: Detection Of Rosmentioning

confidence: 99%

Reactive oxygen species and plant resistance to fungal pathogens

et al. 2015

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Reactive oxygen species (ROS) have been studied for their role in plant development as well as in plant immunity. ROS were consistently observed to accumulate in the plant after the perception of pathogens and microbes and over the years, ROS were postulated to be an integral part of the defence response of the plant. In this article we will focus on recent findings about ROS involved in the interaction of plants with pathogenic fungi. We will describe the ways to detect ROS, their modes of action and their importance in relation to resistance to fungal pathogens. In addition we include some results from works focussing on the fungal interactor and from studies investigating roots during pathogen attack.

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Section: Detection Of Rosmentioning

confidence: 99%

Reactive oxygen species and plant resistance to fungal pathogens

et al. 2015

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“…A genetically encoded redox probe, roGFP (7), recently was used to visualize the redox status in plant leaf cytosol (around Ϫ310 mV) (11) and also in yeast and mammalian cells that were exposed to oxidoreductive reagents, including H 2 O 2 and dithiothreitol (DTT) (4). However, the roGFP probe did not respond to cell treatment with nonoxidative reagents such as ATZ, a catalase inhibitor.…”

Section: Discussionmentioning

confidence: 99%

A Novel Fluorescent Sensor Protein for Visualization of Redox States in the Cytoplasm and in Peroxisomes

Yano

Oku

Akeyama

et al. 2010

Molecular and Cellular Biology

102

101

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Reactive oxygen species are generated within peroxisomes during peroxisomal metabolism. However, due to technological difficulties, the intraperoxisomal redox state remain elusive, and the effect of peroxisome deficiency on the intracellular redox state is controversial. A newly developed, genetically encoded fluorescence resonance energy transfer (FRET) probe, Redoxfluor, senses the physiological redox state via its internal disulfide bonds, resulting in a change in the conformation of the protein leading to a FRET response. We made use of Redoxfluor to measure the redox states at the subcellular level in yeast and Chinese hamster ovary (CHO) cells. In wild-type peroxisomes harboring an intact fatty acid ␤-oxidation system, the redox state within the peroxisomes was more reductive than that in the cytosol, despite the fact that reactive oxygen species were generated within the peroxisomes. Interestingly, we observed that the redox state of the cytosol of cell mutants for peroxisome assembly, regarded as models for a neurological metabolic disorder, was more reductive than that of the wild-type cells in yeast and CHO cells. Furthermore, Redoxfluor was utilized to develop an efficient system for the screening of drugs that moderate the abnormal cytosolic redox state in the mutant CHO cell lines for peroxisome assembly without affecting the redox state of normal cells.

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“…In itself, this observation suggests either that oxidative stress plays a relatively minor role during drought or at least that the antioxidative and reductant-generating systems have sufficient capacity to keep ROS-sensitive redox pools largely in the reduced state. Nevertheless, measurements using a redox-sensitive GFP suggest that the cytosol becomes more oxidized during drought (Jubany-Mari et al, 2010). Evidence suggests that these proteins report mainly on the glutathione redox potential (Meyer et al, 2007), suggesting that drought decreases either the total cytosolic glutathione pool or the GSH:GSSG ratio.…”

Section: Measurable Changes In Redox State During Droughtmentioning

confidence: 99%

The Roles of Reactive Oxygen Metabolism in Drought: Not So Cut and Dried

2014

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Drought is considered to cause oxidative stress, but the roles of oxidant-induced modifications in plant responses to water deficit remain obscure. Key unknowns are the roles of reactive oxygen species (ROS) produced at specific intracellular or apoplastic sites and the interactions between the complex, networking antioxidative systems in restricting ROS accumulation or in redox signal transmission. This Update discusses the physiological aspects of ROS production during drought, and analyzes the relationship between oxidative stress and drought from different but complementary perspectives. We ask to what extent redox changes are involved in plant drought responses and discuss the roles that different ROS-generating processes may play. Our discussion emphasizes the complexity and the specificity of antioxidant systems, and the likely importance of thiol systems in drought-induced redox signaling. We identify candidate drought-responsive redox-associated genes and analyze the potential importance of different metabolic pathways in drought-associated oxidative stress signaling.

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Use of a redox‐sensing GFP (c‐roGFP1) for real‐time monitoring of cytosol redox status in Arabidopsis thaliana water‐stressed plants

Cited by 61 publications

References 29 publications

Reactive oxygen species and plant resistance to fungal pathogens

Reactive oxygen species and plant resistance to fungal pathogens

A Novel Fluorescent Sensor Protein for Visualization of Redox States in the Cytoplasm and in Peroxisomes

The Roles of Reactive Oxygen Metabolism in Drought: Not So Cut and Dried

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