Use of a rapid microwave fixation technique for immunocytochemical demonstration of tumor necrosis factor, interleukin-1.ALPHA., and interluekin-1.BETA. in activated human peripheral mononuclear cells.

Haruna, Nana-Fatima D; Monden, Takushi; Morimoto, Hideki; Murotani, M; Yagyu, Tomohiko; Nagaoka, Hirohito; Shimano, Takashi; Mori, Takesada

doi:10.1267/ahc.23.563

Cited by 18 publications

(5 citation statements)

References 13 publications

(1 reference statement)

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“…The streptococcal preparation OK-432 is an immunostimulator produced by treating the Su strain of Streptococcus pyogenes with heat and penicillin, and it has been recognised as one of the most effective biological response modifiers (BRMs) for augmenting host immunity Torisu et al, 1983;Uchida et al, 1984;Watanabe & Iwa, 1987;Haruna et al, 1990). We have previously shown that the anti-tumour activity of OK-432 is considerably enhanced by intratumoral injection together with fibrinogen .…”

mentioning

confidence: 99%

Analysis of cytotoxic activity of the CD4+ T lymphocytes generated by local immunotherapy

Katsumoto

Monden²,

Takeda³

et al. 1996

Br J Cancer

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Summary We previously reported that the anti-tumour effect of OK-432 is considerably enhanced by its intratumoral injection together with fibrinogen. In the present study, we generated killer T cells by culturing tumour-infiltrating lymphocytes from thyroid cancer patients who had received this local immunotherapy. Phenotypic analysis revealed that the T cells were positive for CD3+, CD4+, Leu8-, CD45RO+ and T-cell

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mentioning

confidence: 99%

Analysis of cytotoxic activity of the CD4+ T lymphocytes generated by local immunotherapy

Katsumoto

Monden²,

Takeda³

et al. 1996

Br J Cancer

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“…One favoring tumor growth includes: 1) production of protease and plasminogen activator, which contribute to tissue invasion by tumor cells [26]; 2) angiogenic activity which promotes the formation of new vessels [27]; and 3) production of prostaglandins which suppress antitumor immunological defenses [28,29]. The other favoring tumor regression includes: 1) production of TNF-0, which is cytotoxic for tumor cells [4,30]; 2) production of IL-1, which promotes antitumor immunological defenses [4]; and 3) phagocytosis and antigen presentation, which are essential for antitumor immunological responses.…”

Section: Discussionmentioning

confidence: 99%

“…OK-432, an attenuated strain of Streptococcus pyogenes, has been recognized as one of the most effective biological response modifiers (BRMs) [1][2][3][4][5][6][7][8][9]. OK-432 induced a markedly augmented antitumor effect on colorectal cancers when injected intratumorally together with fibrinogen [10].…”

Section: Introductionmentioning

confidence: 99%

The effect of local immunotherapy for breast cancer using a mixture of OK-432 and fibrinogen supplemented with activated macrophages

et al. 1993

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OK-432 is an immunomodulatory agent prepared from a strain of Streptococcus pyogenes. We have previously reported that intratumoral injection of a mixture of OK-432 and fibrinogen (hereinafter referred to as OK/fbg) is very effective in the local immunotherapy for colorectal cancer. However, we found that the intratumoral injection of OK/fbg into tumor tissues of breast cancers did not always induce a strong antitumor effect. With conventional OK/fbg treatment, tumor necrosis observed in breast cancer tumors was significantly less than that in colorectal cancer tumors; the formation of fibrin meshwork and macrophage infiltration, in particular, were poor. In this study, the OK/fbg mixture was supplemented with activated macrophages for local immunotherapy of breast cancers. Macrophages were prepared from peripheral blood of breast cancer patients and activated with 0.05 mg/ml of OK-432. Between 2-7 days before operation, a single intratumoral injection of the above mixtures was done. The addition of activated macrophages to the OK/fbg mixture resulted in marked degrees of fibrin meshwork formation, macrophage infiltration and cancer cell necrosis. These findings suggest that the recruitment of macrophages in tumor stroma and their activation are necessary for sufficient induction of antitumor immunity, and supplementation of activated macrophages at the site of immune reaction may be an alternative method for reinforcement of the antitumor effect of local immunotherapy.

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“…Immunogold-silver staining in plastic sections also can be accelerated by exposure to M W s (Wannakrairot and Leong 1989). MW irradiation has also been employed to immobilise various biologic substances, such as interleukin-1 and tumour necrosis factor (Haruna et al 1990) and p53 (Kawasaki et al 1992). Shi et al (1991 recently described a method of antigen retrieval by MW heating of tissue sections to temperatures of up to 100°C in the presence of metal solutions, particularly lead.…”

Section: Microwave Irradiation and Immunostainingmentioning

confidence: 99%

Microwave techniques for diagnostic laboratories

Leong

1993

Scanning

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Microwaves (MWs) were first introduced as a method of fixation just over 20 years ago. In recent years their use has extended far beyond that of a safe, clean and rapid method of fixation of tissue blocks and large specimens, including brains. MWs accelerate the action of cross-linking fixatives and can greatly accelerate the various stages of tissue processing to produce a paraffin block in 30 min. An extensive range of ultrafast MW-stimulated special stains has been developed, and immunohistochemical procedures can be completed in 20 min by employing MWs. Cellular antigens are distinctly better preserved in tissues fixed by MWs than by conventional cross-linking fixatives. Also, the cytomorphology of cryostat sections irradiated in Wolman's solution is clearly improved. MWs can similarly be applied for fixation and staining of preparations for transmission and scanning electron microscopy, and they also greatly accelerate polymerisation of resins. In the current climate of cost containment, this wide range of applications makes the MW oven an invaluable addition to the diagnostic laboratory.

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Use of a rapid microwave fixation technique for immunocytochemical demonstration of tumor necrosis factor, interleukin-1.ALPHA., and interluekin-1.BETA. in activated human peripheral mononuclear cells.

Cited by 18 publications

References 13 publications

Analysis of cytotoxic activity of the CD4+ T lymphocytes generated by local immunotherapy

Analysis of cytotoxic activity of the CD4+ T lymphocytes generated by local immunotherapy

The effect of local immunotherapy for breast cancer using a mixture of OK-432 and fibrinogen supplemented with activated macrophages

Microwave techniques for diagnostic laboratories

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