Use of a Polymerase Chain Reaction for Detection of <i>Mycoplasma Dispar</i> in the Nasal Mucus of Calves

Marques, Lucas Miranda; Buzinhani, Melissa; Yamaguti, Maurício; Oliveira, Renata Cristina Gobbi de; Ferreira, Juliana Bonin; Mettifogo, Elena; Timenetsky, Jorge

doi:10.1177/104063870701900118

Cited by 16 publications

(16 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Field samples giving different results in DGGE and DNA microarray assay were additionally examined using species-specific PCR protocols from the literature for M. bovis [23], M. bovirhinis , M. alkalescens [24], M. dispar [25], M. ovipneumoniae [26], and M. arginini [27].…”

Section: Methodsmentioning

confidence: 99%

A Novel Rapid DNA Microarray Assay Enables Identification of 37 Mycoplasma Species and Highlights Multiple Mycoplasma Infections

et al. 2012

View full text Add to dashboard Cite

Mycoplasmas comprise a conglomerate of pathogens and commensals occurring in humans and animals. The genus Mycoplasma alone contains more than 120 species at present, and new members are continuously being discovered. Therefore, it seems promising to use a single highly parallel detection assay rather than develop separate tests for each individual species. In this study, we have designed a DNA microarray carrying 70 oligonucleotide probes derived from the 23S rRNA gene and 86 probes from the tuf gene target regions. Following a PCR amplification and biotinylation step, hybridization on the array was shown to specifically identify 31 Mycoplasma spp., as well as 3 Acholeplasma spp. and 3 Ureaplasma spp. Members of the Mycoplasma mycoides cluster can be recognized at subgroup level. This procedure enables parallel detection of Mollicutes spp. occurring in humans, animals or cell culture, from mono- and multiple infections, in a single run. The main advantages of the microarray assay include ease of operation, rapidity, high information content, and affordability. The new test's analytical sensitivity is equivalent to that of real-time PCR and allows examination of field samples without the need for culture. When 60 field samples from ruminants and birds previously analyzed by denaturing-gradient gel electrophoresis (DGGE) were tested by the microarray assay both tests identified the same agent in 98.3% of the cases. Notably, microarray testing revealed an unexpectedly high proportion (35%) of multiple mycoplasma infections, i.e., substantially more than DGGE (15%). Two of the samples were found to contain four different Mycoplasma spp. This phenomenon deserves more attention, particularly its implications for epidemiology and treatment.

show abstract

Section: Methodsmentioning

confidence: 99%

A Novel Rapid DNA Microarray Assay Enables Identification of 37 Mycoplasma Species and Highlights Multiple Mycoplasma Infections

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Several researches demonstrated that sampling by BALF was more useful for prediction of lower respiratory airway pathogens than nasal swabs although clearly not as convenient [19,20]. Therefore, in this study, PCRs based on 16S rRNA genes amplified M. bovis DNA [15,21] and were used to confirm Mycoplasma bronchopneumonia, using BALF samples.…”

Section: Discussionmentioning

confidence: 98%

Analysis of Trace and Major Elements in Bronchoalveolar Lavage Fluid of Mycoplasma Bronchopneumonia in Calves

Suzuki

Higuchi

Iwano

et al. 2011

Biol Trace Elem Res

View full text Add to dashboard Cite

The aim of this study was to evaluate the reliability and effectiveness of direct determination of trace and major element concentrations in bronchoalveolar lavage fluid samples from Holstein calves with Mycoplasma bronchopneumonia (n = 21) and healthy controls (n = 20). The samples were obtained during bronchoscopy using a standard examination method. A total of 18 elements (aluminum, bromine, calcium, chlorine, chromium, copper, iron, potassium, magnesium, manganese, molybdenum, nickel, phosphorous, sulfur, silicon, strontium, titanium, and zinc) were detected by particle-induced X-ray emission. The average bromine, iron, potassium, magnesium, and phosphorous concentrations were higher in calves with bronchopneumonia than in controls (p < 0.05). They were found to have higher amounts of calcium and zinc, and a higher zinc-copper ratio than that in healthy calves (p < 0.001). Based on the receiver operating characteristics curves, we propose a diagnostic cutoff point for zinc-copper ratio for identification of Mycoplasma pneumonia of 8.676. Our results indicate that assessment of the elemental composition of broncholaveolar lavage fluid is a promising diagnostic tool for Mycoplasma bronchopneumonia.

show abstract

“…Healthy calves and cattle can be infected with M. dispar aerogenically from animals that have chronic or subclinical respiratory diseases. According to the scientific literature, M. dispar is often isolated from 3-4 month-old calves suffering from pneumonia (Hirose et al 2003, Marques et al 2007). …”

Section: Discussionmentioning

confidence: 99%

“…This species causes disruption of normal ciliary function in the tracheal epithelium and predisposes the lower respiratory tract to infection with primary lung pathogens. M. dispar is frequently isolated from 3-4 month-old calves with respiratory diseases; however, it may also be detected in healthy animals (Hirose et al 2003, Marques et al 2007. Some of these species are often found as a part of the microbial flora of the upper respiratory tract in healthy calves, and in most reports they have been isolated in mixed infections with other known pathogens .…”

Section: Introductionmentioning

confidence: 99%

Laboratory diagnosis of mycoplasma infection in young cattle

Gabinaitienė¹,

Šiugždaitė²,

Žilinskas³

2011

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

The aim of this study was to detect Mycoplasma species in the respiratory tract of 110, 310 and 510 day-old groups of cattle by serological, bacteriological and histopathological investigations. Antibodies against M. bovis were found in 75% of the 110 day-old, in 50% -of the 310 day-old and in 55% -of the 510 day-old groups of cattle.Bacteriological examination of the samples from nasal cavities revealed that Mycoplasma carriers were found in 60% of the 110 day-old group of cattle, 40% of the 310 day-old and 40% of the 510 day-old group of cattle. Using the PCR method Mycoplasma was isolated from 25% of lung samples of the 510 day-old group of cattle. Mycoplasma bovis and Mycoplasma dispar were confirmed by serological investigations. Foci of bronchointerstitial pneumonia were determined by histopathological examination in 27.5% of lung samples. Mycoplasma bovis was isolated in 72.7% of bronchointerstitial pneumonia cases. Data processing with an SPSS 13.0 statistical package led to the conclusion that Mycoplasma bovis was found more frequently in the 110 day-old group of cattle (the youngest age group in this study) rather than in the 310 and 510 day-old groups of cattle (χ 2 = 6.531; p = 0.038). The results obtained led to the conclusion that serological, bacteriological and histopathological examinations are important in detecting particular animal -carriers of Mycoplasma.

show abstract

Use of a Polymerase Chain Reaction for Detection of Mycoplasma Dispar in the Nasal Mucus of Calves

Cited by 16 publications

References 9 publications

A Novel Rapid DNA Microarray Assay Enables Identification of 37 Mycoplasma Species and Highlights Multiple Mycoplasma Infections

A Novel Rapid DNA Microarray Assay Enables Identification of 37 Mycoplasma Species and Highlights Multiple Mycoplasma Infections

Analysis of Trace and Major Elements in Bronchoalveolar Lavage Fluid of Mycoplasma Bronchopneumonia in Calves

Laboratory diagnosis of mycoplasma infection in young cattle

Contact Info

Product

Resources

About