Use of a gastrointestinal model to assess potential amino acid bioavailability in diets for rainbow trout (Oncorrhynchus mykiss)

Márquez, Lorenzo; Øverland, Margareth; Martínez‐Llorens, Silvia; Morken, Thea; Moyano, Francisco Javier

doi:10.1016/j.aquaculture.2012.12.008

Cited by 13 publications

(16 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The consistency of in vitro techniques employing species-specific digestive enzymes depends on the standardization of the hydrolytic capacity of the recovered extract. Most commonly, extracts have been standardized to the activities of trypsin and/or chymotrypsin (Chong et al, 2002;Grabner, 1985;Tibbetts et al, 2011a), or total alkaline proteinase (El-Sayed et al, 2000;Hamdan et al, 2009;Lemos et al, 2009;Márquez et al, 2013). The present study introduces the degree of protein hydrolysis (DH) as a standardization method using analytical grade proteinaceous substrates under the same conditions (pH-stat) as the in vitro digestion assays.…”

Section: Discussionmentioning

confidence: 99%

“…The use of species-specific crude enzyme extracts for in vitro digestion methods may be important since catalytic output may differ significantly among species Hamdan et al, 2009;Lemos et al, 2004;Márquez et al, 2013) and the donor organism (weight, age, feeding status, farming system) and habitat (water salinity, natural productivity) Lemos and Nunes, 2008). The consistency of in vitro techniques employing species-specific digestive enzymes depends on the standardization of the hydrolytic capacity of the recovered extract.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Species specific in vitro protein digestion (pH-stat) for fish: method development and application for juvenile rainbow trout (Oncorhynchus mykiss), cobia (Rachycentron canadum), and Nile tilapia (Oreochromis niloticus)

Yasumaru

Lemos

2014

Aquaculture

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Species specific in vitro protein digestion (pH-stat) for fish: method development and application for juvenile rainbow trout (Oncorhynchus mykiss), cobia (Rachycentron canadum), and Nile tilapia (Oreochromis niloticus)

Yasumaru

Lemos

2014

Aquaculture

View full text Add to dashboard Cite

“…The results of this method are expected to give a good correlation with the apparent digestibility from in vivo experiments (Dimes and Haard ). In this technique, the degree of protein hydrolysis is estimated through soluble protein and FAA quantitative determination (Morales and Moyano ; Márquez et al ) present in the supernatant.…”

mentioning

confidence: 99%

Comparative Characterization of Enzymatic Digestion from Fish and Soybean Meal from Simulated Digestive Process of Pacific Bluefin Tuna, Thunnus orientalis

Román-Gavilanes¹,

Martínez‐Montaño

Viana³

2015

J World Aquaculture Soc

View full text Add to dashboard Cite

The digestive process of the Pacific bluefin tuna (PBT), Thunnus orientalis, was simulated through two phases of in vitro digestion: acidic digestion with porcine pepsin, followed by alkaline digestion with pancreatic crude extract (PCE) obtained from the PBT to hydrolyze fish meal (FM) and soybean meal (SBM) as protein substrates. The crude protein from FM resulted in a lower degree of hydrolysis (73.3%) compared with SBM (79.2%). However, the resulting digested products showed that FM contained 35% more small peptides, with sizes <6.5 kDa than those from the starting material (>150 kDa). The SBM had an increase of only 1.3% in the similar peptide cut-offs found after hydrolysis. These results suggested that FM appeared to be a better source of protein according to the amount of low-molecular weight peptides. In addition, the proteolytic activity of PCE showed that 88.9% of its alkaline proteolytic activity corresponded to trypsin and 2.9% corresponded to chymotrypsin activity. The results shown here demonstrate that peptide sizes are important in identifying suitable protein sources for aquafeed production to reinforce the primary results obtained from the in vitro digestibility using the pH-Stat system. These results also contribute to a better understanding of the digestibility process in aquatic organisms.

show abstract

“…The consistency of in vitro techniques employing species-specific digestive enzymes depends on the standardization of the hydrolytic capacity of the recovered extract. Most commonly, extracts have been standardized to the activities of trypsin and or chymotrypsin (BASSOMPIERRRE, et al, 1997b;CHONG et al, 2002;HAARD, 1994;GRABNER, 1985;RUNGRUANGSAK-TORRISSEN, et al, 2002;TIBBETTS et al, 2011a), or total alkaline proteinase (ALARCÓN, et al, 2002;EL-SAYED, et al, 2000;EZQUERRA et al, 1997;HAMDAN, et al, 2009;LEMOS et al, 2009;MÁRQUEZ et al, 2013). The present study introduces the degree of protein hydrolysis (DH) as a standardization method using analytical grade proteinaceous substrates under the same conditions (pH-stat) as of the in vitro digestion assays.…”

Section: Discussionmentioning

confidence: 99%

“…The use of species-specific crude enzyme extracts for in vitro digestion methods may be important since catalytic output may differ significantly among species (DIMES et al, 1994b;HAMDAN et al, 2009;LEMOS et al, 2004;MÁRQUEZ et al, 2013) and the donor organism (weight, age, feeding status, farming system) and habitat (water salinity, natural productivity) (BASSOMPIERRE, et al, 1998;HAARD, 1994;NUNES, 2008;RUNGRUANGSAK-TORRISSEN et al, 2002). The consistency of in vitro techniques employing species-specific digestive enzymes depends on the standardization of the hydrolytic capacity of the recovered extract.…”

Section: Discussionmentioning

confidence: 99%

Digestão in vitro de proteína e fósforo utilizando enzimas digestivas espécie-específicas: potencial de desenvolvimento e aplicação para a aquicultura de espécies de peixes

Yasumaru¹

View full text Add to dashboard Cite

習う一生Learning is a lifetime. Esta tese teve como objetivo o desenvolvimento de método in vitro pH-stat com enzimas espécie-específicas, avaliando a digestão de proteína (PB) e fósforo (P) em peixes, para prever a digestibilidade in vivo. As espécies utilizadas como modelos experimentais foram a truta arco-íris, Oncorhynchus mykiss, o bijupirá, Rachycentron canadum e a tilápia-do-Nilo, Oreochromis niloticus. Extratos enzimáticos foram obtidos de estômago, cecos pilóricos (truta e bijupirá) ou intestino (tilápia) de indivíduos de diferentes tamanhos e estado alimentar. A capacidade hidrolítica das enzimas foi padronizada utilizando substratos protéicos de grau analítico por meio da determinação do grau de hidrólise protéica (DH) em ensaio pH-stat. Ingredientes práticos foram hidrolisados com extrato enzimático de (i) estômago, (ii) cecos pilóricos (truta e bijupirá)/intestino (tilápia) ou (iii) estômago seguido de cecos pilóricos/intestino (dupla hidrólise) para determinar os valores de DH. O método de determinação de DH apresenta baixo coeficiente de variação, e pode ser uma ferramenta útil no ranqueamento e no controle de qualidade de ingredientes práticos. O P solúvel liberado de amostras de nove rações comerciais para tilápia, com níveis de garantia similares (32% proteína bruta, 4-6 mm) submetidos à digestão in vitro com extrato de estômago, intestino ou dupla hidrólise foram avaliados. Os extratos enzimáticos foram obtidos de tilápias cultivadas em condições comerciais em tanque-rede. Liberação de P solúvel foi determinada em amostras incubadas somente em água destilada (pH 6.4 ± 0.4), em água destilada a pH 2.0, pH 8.0, e sequencialmente em pH 2.0 e 8.0. Amostras também foram incubadas nesses valores de pH e incluindo os extratos enzimáticos de estômago e intestino separada e sequencialmente (dupla hidrólise). A liberação de P solúvel após digestão do estômago foi maior do que após digestão do intestino ou após dupla hidrólise. A digestibilidade do P parece estar relacionada mais ao pH do meio do que à hidrólise enzimática. Correlações significativas (P<0,05) foram observadas entre P total das rações e o P solúvel liberado em água destilada, e entre P total da ração e P liberado após digestão intestinal. O método in vitro apresentou baixo coeficiente de variação (< 5% c.v.). Coeficientes de digestibilidade aparente (CDA) de PB e P de nove rações comerciais para tilápia foram avaliados in vivo utilizando cinzas insolúveis em ácido como marcador interno. O ensaio foi feito em laboratório com tilápias em fase de crescimento simulando condições comerciais de estocagem. Foram avaliados também o crescimento e correlações entre CDA in vivo e DH in vitro de PB e CDA P in vivo e liberação in vitro de P solúvel. Não houve diferença no ganho de peso entre as rações (P>0,05). Valores de DH não apresentaram correlação significativa (P>0,05) com CDA PB, mas foi possível discriminar entre os maiores e menores valores de CDA PB. A liberação in vitro de P solúvel em água destilada e após digestão intestinal demostraram corr...

show abstract

Use of a gastrointestinal model to assess potential amino acid bioavailability in diets for rainbow trout (Oncorrhynchus mykiss)

Cited by 13 publications

References 65 publications

Species specific in vitro protein digestion (pH-stat) for fish: method development and application for juvenile rainbow trout (Oncorhynchus mykiss), cobia (Rachycentron canadum), and Nile tilapia (Oreochromis niloticus)

Species specific in vitro protein digestion (pH-stat) for fish: method development and application for juvenile rainbow trout (Oncorhynchus mykiss), cobia (Rachycentron canadum), and Nile tilapia (Oreochromis niloticus)

Comparative Characterization of Enzymatic Digestion from Fish and Soybean Meal from Simulated Digestive Process of Pacific Bluefin Tuna, Thunnus orientalis

Digestão in vitro de proteína e fósforo utilizando enzimas digestivas espécie-específicas: potencial de desenvolvimento e aplicação para a aquicultura de espécies de peixes

Contact Info

Product

Resources

About