Comparative Characterization of Enzymatic Digestion from Fish and Soybean Meal from Simulated Digestive Process of Pacific Bluefin Tuna, <i>Thunnus orientalis</i>

Román-Gavilanes, Ariana I.; Martínez‐Montaño, Emmanuel; Viana, Marı́a Teresa

doi:10.1111/jwas.12204

Cited by 5 publications

(5 citation statements)

References 37 publications

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“…Tisochrysis lutea (TIS), Nannochloropsis gaditana (NAN), Tetraselmis suecica (TET), and Scenedesmus almeriensis (SCE). amount of amino acids released and, therefore, this factor could represent a crucial aspect for the bioavailability and further absorption of free amino acids into the enterocytes [48]. Additionally, a linear relationship between CPD and free amino acids released was found for all the microalgae evaluated.…”

Section: Tablementioning

confidence: 81%

Differential hydrolysis of proteins of four microalgae by the digestive enzymes of gilthead sea bream and Senegalese sole

et al. 2019

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This study evaluates the in vitro protein hydrolysis of four microalgae (Tisochrysis lutea, Nannochloropsis gaditana, Tetraselmis suecica and Scenedesmus almeriensis) by intestinal proteases of gilthead sea bream (Sparus aurata) and Senegalese sole (Solea senegalensis). The hydrolysis of protein was monitored at different sampling times by electrophoretic techniques, and the quantification of the free amino acids released by proteases. Overall, S. aurata or S. senegalensis proteases hydrolysed microalgae protein in a similar way. The highest hydrolysis values (coefficient of protein degradation, CPD > 70%) were obtained for Tisochrysis and Nannochloropsis biomasses, which showed a progressive and almost complete proteolysis at the end of the in vitro assay. Tetraselmis and Scenedesmus protein was also hydrolysed, but SDS-PAGE revealed that two protein fractions remained virtually intact at the end of the in vitro assay. The final amount of free amino acids released in vitro by the fish digestive enzymes ranged from 9 to 25 g 100 g protein −1 . A linear relationship between CPD and the amount of free amino acids released was found, a fact that suggests that microalgae protein is hydrolysed efficiently by the digestive proteases of both fish species. The present study provides information about the protein availability from selected microalgae, which will aid in the initial evaluation of the microalgae as potential protein sources in feeds of two important farmed fish species.

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Section: Tablementioning

confidence: 81%

Differential hydrolysis of proteins of four microalgae by the digestive enzymes of gilthead sea bream and Senegalese sole

et al. 2019

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“…This method employs HPLC columns that separate molecules based on the molecular weight. Only four studies had employed this analytical technique [ 146 , 147 ]. Using this technique, the author showed that in-vitro digestion of soybean minimally increases the low-molecular-weight peptides (<6.5 kDa) compared to fish meal, while similar molecular weight distribution between hydrolyzates of fish meal and poultry meal was observed [ 117 , 118 , 146 , 147 ] .…”

Section: Methodsmentioning

confidence: 99%

“…Only four studies had employed this analytical technique [ 146 , 147 ]. Using this technique, the author showed that in-vitro digestion of soybean minimally increases the low-molecular-weight peptides (<6.5 kDa) compared to fish meal, while similar molecular weight distribution between hydrolyzates of fish meal and poultry meal was observed [ 117 , 118 , 146 , 147 ] . In-vitro digestion of single-cell protein using rainbow trout digestive enzymes yielded two times higher of products with molecular weight between 250 and 4000 Da compared to products with molecular weight less than 250 Da.…”

Section: Methodsmentioning

confidence: 99%

In-vitro digestion models: a critical review for human and fish and a protocol for in-vitro digestion in fish

et al. 2021

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Digestive systems in human, animals, and fish are biological reactors and membranes to digest food and extract nutrients. Therefore, static and dynamic models of in-vitro digestion systems are developed to study e.g. novel food and feed before in-vivo studies. Such models are well developed for human, but not to the same extent for animals and fish. On the other hand, recent advances in aquaculture nutrition have created several potential fish meal replacements, and the assessment of their nutrient digestibility is critical in the application as a fish meal replacement. Using an in-vitro method, the assessment of an ingredient digestibility could be faster and less expensive compared to using an in-vivo experiment. An in-vitro method has been widely used to assess food nutrient digestibility for humans; however, its application for fish is still in the early stages. Both the human and fish as monogastric vertebrates share similar gastrointestinal systems; thus, the concept from the application for humans could be applied for fish. This review aims to improve the in-vitro digestion protocol for fish by adapting the concept from then study for humans, summarizing the current available in-vitro digestion model developed for human and fish in-vitro digestion study, identifying challenges specifically for fish required to be tackled and suggesting an engineering approach to adapt the human in-vitro gastrointestinal model to fish. Protocols to conduct in-vitro digestion study for fish are then proposed.

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“…The molecular weight distribution (MW) of the CH and FH peptides was determined by Size exclusion chromatography (SEC) using a Varian HPLC system equipped with a diode array detector (DAD) (Varian ™ ProStar) and Galaxy ™ software (Varian, version 1.9.302.952) following the methodology used by Román-Gavilanes et al [39]. 20 µL of the sample was injected at a concentration of 5 mg/ mL.…”

Section: Size Exclusion Chromatography (Sec)mentioning

confidence: 99%

“…The SEC process is based on the fractionation of peptides according to the retention time of the molecules in the particles of the stationary phases, in which the molecules separate from each other according to their molecular size [39]. The distribution of the MW of the CH and FH obtained with the proteases of BP and BK, are shown in Figs.…”

Section: Distribution Of Molecular Weights (Mw) Of Peptides From Hydrmentioning

confidence: 99%

Bromelia karatas and Bromelia pinguin: sources of plant proteases used for obtaining antioxidant hydrolysates from chicken and fish by-products

et al. 2020

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In the present study, we evaluated new sources of plant proteases from fruits of Bromelia karatas (BK) and Bromelia pinguin (BP) to obtain antioxidant hydrolyzates/bioactive peptides (BPs) derived from chicken by-products (CH) and fish by-products (FH). The profile of the peptides was identified by reverse-phase high-resolution liquid chromatography (RP-HPLC) and the size weight distribution by molecular exclusion chromatography (SEC). The hydrolysates obtained with BK in both sources of by-products showed greater antioxidant capacity compared to those obtained with BP, presenting similar or higher values when compared to a commercial plant enzyme. The use of new sources of plant proteases allowed to obtain hydrolysates of hydrophilic character with a high percentage (> 50%) of peptides with molecular weights < 17.5 kDa from chicken and fish by-products. Therefore, based on the results obtained in antioxidant capacity it is possible to consider the hydrolysates as potential ingredients, food additives, and pharmaceutical products.

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Comparative Characterization of Enzymatic Digestion from Fish and Soybean Meal from Simulated Digestive Process of Pacific Bluefin Tuna, Thunnus orientalis

Cited by 5 publications

References 37 publications

Differential hydrolysis of proteins of four microalgae by the digestive enzymes of gilthead sea bream and Senegalese sole

Differential hydrolysis of proteins of four microalgae by the digestive enzymes of gilthead sea bream and Senegalese sole

In-vitro digestion models: a critical review for human and fish and a protocol for in-vitro digestion in fish

Bromelia karatas and Bromelia pinguin: sources of plant proteases used for obtaining antioxidant hydrolysates from chicken and fish by-products

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