USA300 (sequence type 8) has become a major clone of methicillin-resistant Staphylococcus aureus in northern Taiwan

Huang, Yhu-Chering; Chen, Chih‐Jung

doi:10.1016/j.ijantimicag.2022.106534

Cited by 11 publications

(11 citation statements)

References 33 publications

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“…Recent reports from Korea and Taiwan have also suggested that the prevalence of USA300 clones has increased 15 – 17 . Although whole genome sequences of the Korean isolates have not been determined, the isolates from Taiwan consisted of multiple USA300 clones similar to European and American types, but were not associated with ΨUSA300.…”

Section: Discussionmentioning

confidence: 99%

“…Candidate SNPs in all retained genomes were filtered with a base quality of ≥ Q30 and a read depth ≥ 10. The molecular epidemiology of the USA300 strains, including the USA300 isolates from Taiwan, was investigated using the contig data 16 , 17 downloaded from the ENA website. Sequences were aligned to the reference strain USA300_TCH1516 and variants of the contigs using Snippy v4.6.0 42 .…”

Section: Methodsmentioning

confidence: 99%

“…In addition, several USA300 clones have undergone evolution in Japan, including the USA300-LV/J (ST8-IVc/ACME negative) and ΨUSA300 (with a 12 bp deletion in ccrB2 on SCC mec ) strains 13 , 14 . During the same period, the prevalence of USA300 increased in Korea and Taiwan 15 – 17 . These findings indicate that the USA300 strain has undergone dissemination in Japan and other Eastern Asian countries.…”

Section: Introductionmentioning

confidence: 98%

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Emergence of community-associated methicillin-resistant Staphylococcus aureus ΨUSA300 among Japanese people with HIV, resulted from stepwise mutations in 2010s

Shinohara

Uehara

Teruya

et al. 2023

Sci Rep

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Although infection with the methicillin-resistant Staphylococcus aureus (MRSA) clone USA300 is extremely rare in Japan, the uniquely evolved clone ΨUSA300 has been reported in Japan. An outbreak of a distinct USA300 clone was recently reported in an HIV/AIDS referral hospital in Tokyo. The present study investigated the evolutionary origin and genetic diversity of USA300-related clones causing regional outbreaks among people living with HIV (PLWHIV) in Tokyo. MRSA isolates collected from PLWHIV in an HIV/AIDS referral center in Tokyo were subjected to whole-genome sequencing and their genetic features were compared with those of previously described USA300 MRSA genomes. Of the 28 MRSAs isolated in 2016–2019, 23 (82.1%) were identified as USA300, with 22 (95.6%) of the latter identified as ΨUSA300. Although the genomic structure of ΨUSA300 was identical to the structures of reference USA300 strains, one clade (cluster A) was found to have acquired 29 previously identified lineage-specific mutations in a stepwise manner. The estimated divergence dates of ΨUSA300 and Cluster A were 2009 and 2012, respectively. These findings suggested that the ΨUSA300 clone had spread among PLWHIVs in Tokyo in the early 2010s, with stepwise acquisition of lineage-specific nonsynonymous mutations.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Emergence of community-associated methicillin-resistant Staphylococcus aureus ΨUSA300 among Japanese people with HIV, resulted from stepwise mutations in 2010s

Shinohara

Uehara

Teruya

et al. 2023

Sci Rep

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“…USA300, the epidemic CA-MRSA clone in America, was among the most virulent strains ( 24 , 32 ). In a recent study in Taiwan ( 33 ), 92 USA300 isolates were identified among 232 MRSA bloodstream isolates, with a proportion of 39.7%. Compared with the USA300-LAC strain, we found MR370 to be much more virulent.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic Analysis and Virulence Characteristics of Methicillin-Resistant Staphylococcus aureus ST45 in China: a Hyper-Virulent Clone Associated with Bloodstream Infections

Wang

Шен

et al. 2023

mSystems

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Methicillin-resistant Staphylococcus aureus ST45 is epidemic worldwide. This study contributed to the awareness of the Chinese hyper-virulent MRSA ST45 strains and served as a timely reminder of its wide dissemination of clonotypes. Further, we provide novel insights for prevention from the perspective of bloodstream infections. ST45-SCC mec V is a clonotype deserving special attention in China, and we performed genetic and phenotypic analyses for the first time on it.

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“…Later in the century, ST8-MSSA also acquired resistance to methicillin: one of the most successful contemporary CA-MRSA, USA300, belongs to ST8 (23,24). US300 has become endemic in North America and is still finding niches to colonize around the globe, with repeated introductions of USA300 into Europe reported (25)(26)(27)(28)(29). ST22-MRSA-IV, another global lineage, has been reported in many countries since the 1990s.…”

Section: Introductionmentioning

confidence: 99%

Ancient methicillin-resistantStaphylococcus aureus: expanding current knowledge using molecular epidemiological characterization of a Swiss legacy collection

Benvenga

Cuénod

Purushothaman

et al. 2023

Preprint

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Few methicillin-resistantStaphylococcus aureus(MRSA) from the early years of its global emergence have been sequenced. Knowledge about evolutionary factors promoting the success of specific MRSA multi-locus sequence types (MLSTs) remains scarce. We aimed to characterize a legacy MRSA collection isolated from 1965 to 1987 and compare it against publicly available international and local genomes. We accessed 451 ancient (1965-1987) Swiss MRSA isolates, stored in the Culture Collection of Switzerland. We determined phenotypic antimicrobial resistance (AMR) and performed Illumina short-read sequencing on all isolates and long-read sequencing on a selection with Oxford Nanopore Technology. For context, we included 103 publicly available international genomes from 1960 to 1992 and sequenced 1207 modern Swiss MRSA isolates from 2007 to 2022. We analyzed the core genome (cg)MLST and predicted SCCmec cassette types, AMR, and virulence genes. Among the 451 ancient Swiss MRSA isolates, we found 17 sequence types (STs) of which 11 have been previously described. Two STs were novel combinations of known loci and six isolates carried previously unsubmitted MLST alleles, representing five new STs (ST7843, ST7844, ST7837, ST7839, and ST7842). Most isolates (83% 376/451) represented ST247-MRSA-I isolated in the 1960s, followed by ST7844 (6% 25/451), a novel single locus variant (SLV) of ST239. Analysis by cgMLST indicated that isolates belonging to ST7844-MRSA-III cluster within the diversity of ST239-MRSA-IIII. Early MRSA were predominantly from clonal complex (CC) 8. From 1980 to the end of the 20th century we observed that CC22 and CC5 as well as CC8 were present, both locally and internationally. The combined analysis of 1761 ancient and contemporary MRSA isolates across more than 50 years uncovered novel STs and allowed us a glimpse into the lineage flux between Swiss and international MRSA across time.

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USA300 (sequence type 8) has become a major clone of methicillin-resistant Staphylococcus aureus in northern Taiwan

Cited by 11 publications

References 33 publications

Emergence of community-associated methicillin-resistant Staphylococcus aureus ΨUSA300 among Japanese people with HIV, resulted from stepwise mutations in 2010s

Emergence of community-associated methicillin-resistant Staphylococcus aureus ΨUSA300 among Japanese people with HIV, resulted from stepwise mutations in 2010s

Phylogenetic Analysis and Virulence Characteristics of Methicillin-Resistant Staphylococcus aureus ST45 in China: a Hyper-Virulent Clone Associated with Bloodstream Infections

Ancient methicillin-resistantStaphylococcus aureus: expanding current knowledge using molecular epidemiological characterization of a Swiss legacy collection

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