Phylogenetic Analysis and Virulence Characteristics of Methicillin-Resistant Staphylococcus aureus ST45 in China: a Hyper-Virulent Clone Associated with Bloodstream Infections

Wang, Xinyi; Wu, Xiaodong; Шен, Ли; Rao, Lulin; Wang, Bingjie; Zhao, Huabin; Zhang, Jiao; Xiao, Yanghua; Guo, Yinjuan; Xu, Yanling; Chen, Liang; Yu, Fangyou

doi:10.1128/msystems.00029-23

Cited by 2 publications

(1 citation statement)

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“…Gene chp enhances staphylococcal virulence by regulating cell wall synthesis and metabolism, vesicle formation and release, and biofilm formation, all of which affect the growth, attachment and invasion ability of Staphylococcus. 38 In conclusion, the fortuitous elimination of this virulence factor during the transmission process is highly advantageous for foreseeable applications of KYS-164. We note that an investigation conducted by Fernández et al 30 considered the biosafety of various Staphylococcus strains, and examined six different sources of S. warneri .…”

Section: Resultsmentioning

confidence: 97%

The composition of Tibetan kefir grain TKG-Y and the antibacterial potential and milk fermentation ability of S. warneri KYS-164 screened from TKG-Y

Guo,

He,

Cheng

et al. 2024

Food Funct.

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Section: Resultsmentioning

confidence: 97%

The composition of Tibetan kefir grain TKG-Y and the antibacterial potential and milk fermentation ability of S. warneri KYS-164 screened from TKG-Y

Guo,

He,

Cheng

et al. 2024

Food Funct.

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Rapid and sensitive detection of methicillin-resistant Staphylococcus aureus through the RPA-PfAgo system

Chen,

Zhang,

Wei

et al. 2024

Front. Microbiol.

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IntroductionBoth the incidence and mortality rates associated with methicillin-resistant Staphylococcus aureus (MRSA) have progressively increased worldwide. A nucleic acid testing system was developed in response, enabling swift and precise detection of Staphylococcus aureus (S. aureus) and its MRSA infection status. This facilitates improved prevention and control of MRSA infections.MethodsIn this work, we introduce a novel assay platform developed by integrating Pyrococcus furiosus Argonaute (PfAgo) with recombinase polymerase amplification (RPA), which was designed for the simultaneous detection of the nuc and mecA genes in MRSA.ResultsThis innovative approach enables visual MRSA detection within 55 mins, boasting a detection limit of 102 copies/μL. Characterized by its high specificity, the platform accurately identifies MRSA infections without cross-reactivity to other clinical pathogens, highlighting its unique capability for S. aureus infection diagnostics amidst bacterial diversity. Validation of this method was performed on 40 clinical isolates, demonstrating a 95.0% accuracy rate in comparison to the established Vitek2-COMPACT system.DiscussionThe RPA-PfAgo platform has emerged as a superior diagnostic tool, offering enhanced sensitivity, specificity, and identification efficacy for MRSA detection. Our findings underscore the potential of this platform to significantly improve the diagnosis and management of MRSA infection.

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Phylogenetic Analysis and Virulence Characteristics of Methicillin-Resistant Staphylococcus aureus ST45 in China: a Hyper-Virulent Clone Associated with Bloodstream Infections

Cited by 2 publications

References 42 publications

The composition of Tibetan kefir grain TKG-Y and the antibacterial potential and milk fermentation ability of S. warneri KYS-164 screened from TKG-Y

The composition of Tibetan kefir grain TKG-Y and the antibacterial potential and milk fermentation ability of S. warneri KYS-164 screened from TKG-Y

Rapid and sensitive detection of methicillin-resistant Staphylococcus aureus through the RPA-PfAgo system

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