Urine storage under refrigeration preserves the sample in chemical, cellularity and bacteriuria analysis of ACS

Ribeiro, Karen Cristina Barcellos; Serabion, Bruno Rotondo Levenhagem; Nolasco, Eduardo Lima; Vanelli, Chislene Pereira; Mesquita, Harleson Lopes de; Corrêa, José Otávio do Amaral

doi:10.1590/s1676-24442013000600006

Cited by 7 publications

(8 citation statements)

References 6 publications

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“…This approach has been used in other multi-country studies including; the International Population Study on Macronutrients and Blood Pressure [INTERMAP] and the International Study of Sodium, Potassium, and Blood Pressure [INTERSALT] [27]. In the first enumerator areas (EAs) sampled within the Accra region, some calculated salt values were unfeasibly high (> 40 g/d, n = 40,) due to unacceptable storage conditions [30]. A follow-up validation study conducted in n = 67 participants included the collection of repeated 24 h urine samples from the same participants in one EA (Alajo; n = 19), while an additional two non-urine EAs from the larger survey sample were visited as a comparator (Mataheko; n = 24 and Nima; n = 24).…”

Section: Study Design Population and Outcomesmentioning

confidence: 99%

Salt and potassium intake among adult Ghanaians: WHO-SAGE Ghana Wave 3

et al. 2020

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Though Ghana has high hypertension prevalence, the country lacks current national salt consumption data required to build and enhance advocacy for salt reduction. We explored the characteristics of a randomly selected sub sample that had valid urine collection, along with matched survey, anthropometric and BP data (n = 839, mean age = 60y), from the World Health Organization’s Study on global AGEing and adult health (WHO-SAGE), Ghana Wave 3, n = 3053). We also investigated the relationship between salt intake and blood pressure (BP) among the cohort. BP was measured in triplicate and 24 h urine was collected for the determination of urinary sodium (Na), potassium (K), creatinine (Cr) and iodine levels. Hypertension prevalence was 44.3%. Median salt intake was 8.3 g/day, higher in women compared to men (8.6, interquartile range (IQR) 7.5 g/day vs 7.5, IQR 7.4 g/day, p < 0.01), younger participants (18–49 y) compared to older ones (50+ y) (9.7, IQR 7.9 g/day vs 8.1, IQR 7.1 g/day, p < 0.01) and those with higher Body Mass Index (BMI) (> 30 kg/m2) compared to a healthy BMI (18.5–24.9 kg/m2) (10.04, IQR 5.1 g/day vs 6.2, IQR 5.6 g/day, p < 0.01). More than three quarters (77%, n = 647) of participants had salt intakes above the WHO maximum recommendation of 5 g/d, and nearly two thirds (65%, n = 548) had daily K intakes below the recommended level of 90 mmol. Dietary sodium to potassium (Na: K) ratios above 2 mmol/mmol were positively associated with increasing BP with age. Population-based interventions to reduce salt intake and increase K consumption are needed.

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Section: Study Design Population and Outcomesmentioning

confidence: 99%

Salt and potassium intake among adult Ghanaians: WHO-SAGE Ghana Wave 3

et al. 2020

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“…Human urine and stent samples must be partially processed within 4–6 h of collection prior to their frozen storage in order to optimize downstream DNA yield ( Jung et al., 2019 , Bundgaard-Nielsen et al., 2020 ). Importantly, urine samples should not be refrigerated prior to their processing due to increased precipitation of urinary sediments, which has been shown to decrease DNA yield ( Ribeiro et al., 2013 , Ackerman et al., 2019 ). DNA extraction should be performed together once all samples are collected to minimize batch effects.…”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

Processing human urine and ureteral stents for 16S rRNA amplicon sequencing

Burton

2021

STAR Protocols

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Summary Ureteral stents are commonly used medical devices that harbor a unique and patient-specific microbial community. This protocol describes an optimized procedure for high-quality DNA extraction from both urine and ureteral stent samples for the purpose of downstream microbiota characterization by amplicon sequencing. Detailed instruction is provided for 16S rRNA gene V4 region sequencing with the Illumina platform, which enables accurate and reproducible microbiota profiling of low bacterial abundance urine and stent samples. For complete details on the use and execution of this protocol, please refer to Al et al. (2020) .

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“…(1) O exame qualitativo de urina (EQU) está entre os exames mais solicitados aos laboratórios de análises clínicas, sendo realizado em três etapas distintas: a análise física, química e microscópica avaliando a cor, densidade, aspecto, presença de leucócitos, bactérias, sangue, glicose, urobilinogênio, bilirrubina, nitrito e sedimentos urinários. (2)(3)(4) Este exame possui baixo custo e é realizado de maneira rápida, confiável, precisa e segura, sendo amplamente utilizado para diagnóstico de patologias, triagens de populações assintomáticas, acompanhamento de doenças e verificação da eficácia do tratamento, principalmente de doenças do trato urinário. (3,5) Nos laboratórios de análises clínicas, todos os procedimentos, incluindo exames de urina, são divididos em fase pré-analítica, analítica e pós-analítica.…”

Section: Introductionunclassified

“…(2)(3)(4) Este exame possui baixo custo e é realizado de maneira rápida, confiável, precisa e segura, sendo amplamente utilizado para diagnóstico de patologias, triagens de populações assintomáticas, acompanhamento de doenças e verificação da eficácia do tratamento, principalmente de doenças do trato urinário. (3,5) Nos laboratórios de análises clínicas, todos os procedimentos, incluindo exames de urina, são divididos em fase pré-analítica, analítica e pós-analítica.…”

Section: Introductionunclassified